Effects of annexins II and V on survival of neurons and astrocytes in vitro

Shu Han, Kai Hua Zhang, Pei Hua Lu, Xiao-Ming Xu

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

AIM: To study the effects of annexins II and V on the survival and neurite outgrowth of primary cultured neurons and the survival of astrocytes after peroxide and hypoxia insults in vitro. METHODS: Annexins II and V proteins and/or corresponding antibodies were added to the medium of primary neocortical cultures. H2O2 and NaN3 were used to induce neuron injury, respectively. Lactate dehydrogenase (LDH) release was measured. RESULTS: Addition of annexin II or V into the culture medium did not affect the normal survival and neurite outgrowth of cortical neurons. However, when an antibody against annexin II or V was added to the culture, the survival and neurite outgrowth of these neurons markedly declined. Further, addition of the two annexins into cortical cultures after peroxide and hypoxia insults markedly reduced the LDH release and cell death. CONCLUSION: Annexins II and V are essential for the survival and neurite outgrowth of developing cortical neurons, the survival of glial cells, and protect neurons and glial cells against peroxide and hypoxia injuries.

Original languageEnglish (US)
Pages (from-to)602-610
Number of pages9
JournalActa Pharmacologica Sinica
Volume25
Issue number5
StatePublished - May 2004
Externally publishedYes

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Annexin A2
Annexin A5
Astrocytes
Neurons
Peroxides
L-Lactate Dehydrogenase
Neuroglia
Annexins
Sodium Azide
Antibodies
Wounds and Injuries
Cell death
Culture Media
In Vitro Techniques
Cell Death
Neuronal Outgrowth

Keywords

  • Annexin II
  • Annexin V
  • Astrocytes
  • Cell culture
  • Hydrogen peroxide
  • Hypoxia
  • Lactate dehydrogenases
  • Neurons

ASJC Scopus subject areas

  • Chemistry(all)
  • Pharmacology

Cite this

Effects of annexins II and V on survival of neurons and astrocytes in vitro. / Han, Shu; Zhang, Kai Hua; Lu, Pei Hua; Xu, Xiao-Ming.

In: Acta Pharmacologica Sinica, Vol. 25, No. 5, 05.2004, p. 602-610.

Research output: Contribution to journalArticle

Han, Shu ; Zhang, Kai Hua ; Lu, Pei Hua ; Xu, Xiao-Ming. / Effects of annexins II and V on survival of neurons and astrocytes in vitro. In: Acta Pharmacologica Sinica. 2004 ; Vol. 25, No. 5. pp. 602-610.
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