Effects of broad frequency vibration on cultured osteoblasts

Shigeo M. Tanaka, Jiliang Li, Randall L. Duncan, Hiroki Yokota, David Burr, Charles H. Turner

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Bone is subjected in vivo to both high amplitude, low frequency strain, incurred by locomotion, and to low amplitude, broad frequency strain. The biological effects of low amplitude, broad frequency strain are poorly understood. To evaluate the effects of low amplitude strains ranging in frequency from 0 to 50Hz on osteoblastic function, we seeded MC3T3-E1 cells into collagen gels and applied the following loading protocols for 3min per day for either 3 or 7 days: (1) sinusoidal strain at 3Hz, with 0-3000μstrain peak-to-peak followed by 0.33s resting time, (2) "broad frequency vibration" of low amplitude strain (standard deviation of 300μstrain) including frequency components from 0 to 50Hz, and (3) sinusoidal strain combined with broad frequency vibration (S+V). The cells were harvested on day 4 or 8. We found that the S+V stimulation significantly repressed cell proliferation by day 8. Osteocalcin mRNA was up-regulated 2.6-fold after 7 days of S+V stimulation, and MMP-9 mRNA was elevated 1.3-fold after 3 days of vibration alone. Sinusoidal stimulation alone did not affect the cell responses. No differences due to loading were observed in alkaline phosphatase activity and in mRNA levels of type I collagen, osteopontin, connexin 43, MMPs-1A, -3, -13. These results suggest that osteoblasts are more sensitive to low amplitude, broad frequency strain, and this kind of strain could sensitize osteoblasts to high amplitude, low frequency strain. This suggestion implies a potential contribution of stochastic resonance to the mechanical sensitivity of osteoblasts.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalJournal of Biomechanics
Volume36
Issue number1
DOIs
StatePublished - Jan 1 2003

Fingerprint

Osteoblasts
Vibration
Matrix Metalloproteinases
Messenger RNA
Connexin 43
Osteopontin
Osteocalcin
Locomotion
Collagen Type I
Alkaline Phosphatase
Collagen
Gels
Cell Proliferation
Bone and Bones
Phosphatases
Cell proliferation
Bone

Keywords

  • Biomechanics
  • Bone
  • Mechanical vibration
  • Osteoblasts
  • Osteoporosis

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Tanaka, S. M., Li, J., Duncan, R. L., Yokota, H., Burr, D., & Turner, C. H. (2003). Effects of broad frequency vibration on cultured osteoblasts. Journal of Biomechanics, 36(1), 73-80. https://doi.org/10.1016/S0021-9290(02)00245-2

Effects of broad frequency vibration on cultured osteoblasts. / Tanaka, Shigeo M.; Li, Jiliang; Duncan, Randall L.; Yokota, Hiroki; Burr, David; Turner, Charles H.

In: Journal of Biomechanics, Vol. 36, No. 1, 01.01.2003, p. 73-80.

Research output: Contribution to journalArticle

Tanaka, SM, Li, J, Duncan, RL, Yokota, H, Burr, D & Turner, CH 2003, 'Effects of broad frequency vibration on cultured osteoblasts', Journal of Biomechanics, vol. 36, no. 1, pp. 73-80. https://doi.org/10.1016/S0021-9290(02)00245-2
Tanaka, Shigeo M. ; Li, Jiliang ; Duncan, Randall L. ; Yokota, Hiroki ; Burr, David ; Turner, Charles H. / Effects of broad frequency vibration on cultured osteoblasts. In: Journal of Biomechanics. 2003 ; Vol. 36, No. 1. pp. 73-80.
@article{dd9e23d366cb4839b881be9aca93862c,
title = "Effects of broad frequency vibration on cultured osteoblasts",
abstract = "Bone is subjected in vivo to both high amplitude, low frequency strain, incurred by locomotion, and to low amplitude, broad frequency strain. The biological effects of low amplitude, broad frequency strain are poorly understood. To evaluate the effects of low amplitude strains ranging in frequency from 0 to 50Hz on osteoblastic function, we seeded MC3T3-E1 cells into collagen gels and applied the following loading protocols for 3min per day for either 3 or 7 days: (1) sinusoidal strain at 3Hz, with 0-3000μstrain peak-to-peak followed by 0.33s resting time, (2) {"}broad frequency vibration{"} of low amplitude strain (standard deviation of 300μstrain) including frequency components from 0 to 50Hz, and (3) sinusoidal strain combined with broad frequency vibration (S+V). The cells were harvested on day 4 or 8. We found that the S+V stimulation significantly repressed cell proliferation by day 8. Osteocalcin mRNA was up-regulated 2.6-fold after 7 days of S+V stimulation, and MMP-9 mRNA was elevated 1.3-fold after 3 days of vibration alone. Sinusoidal stimulation alone did not affect the cell responses. No differences due to loading were observed in alkaline phosphatase activity and in mRNA levels of type I collagen, osteopontin, connexin 43, MMPs-1A, -3, -13. These results suggest that osteoblasts are more sensitive to low amplitude, broad frequency strain, and this kind of strain could sensitize osteoblasts to high amplitude, low frequency strain. This suggestion implies a potential contribution of stochastic resonance to the mechanical sensitivity of osteoblasts.",
keywords = "Biomechanics, Bone, Mechanical vibration, Osteoblasts, Osteoporosis",
author = "Tanaka, {Shigeo M.} and Jiliang Li and Duncan, {Randall L.} and Hiroki Yokota and David Burr and Turner, {Charles H.}",
year = "2003",
month = "1",
day = "1",
doi = "10.1016/S0021-9290(02)00245-2",
language = "English",
volume = "36",
pages = "73--80",
journal = "Journal of Biomechanics",
issn = "0021-9290",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Effects of broad frequency vibration on cultured osteoblasts

AU - Tanaka, Shigeo M.

AU - Li, Jiliang

AU - Duncan, Randall L.

AU - Yokota, Hiroki

AU - Burr, David

AU - Turner, Charles H.

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Bone is subjected in vivo to both high amplitude, low frequency strain, incurred by locomotion, and to low amplitude, broad frequency strain. The biological effects of low amplitude, broad frequency strain are poorly understood. To evaluate the effects of low amplitude strains ranging in frequency from 0 to 50Hz on osteoblastic function, we seeded MC3T3-E1 cells into collagen gels and applied the following loading protocols for 3min per day for either 3 or 7 days: (1) sinusoidal strain at 3Hz, with 0-3000μstrain peak-to-peak followed by 0.33s resting time, (2) "broad frequency vibration" of low amplitude strain (standard deviation of 300μstrain) including frequency components from 0 to 50Hz, and (3) sinusoidal strain combined with broad frequency vibration (S+V). The cells were harvested on day 4 or 8. We found that the S+V stimulation significantly repressed cell proliferation by day 8. Osteocalcin mRNA was up-regulated 2.6-fold after 7 days of S+V stimulation, and MMP-9 mRNA was elevated 1.3-fold after 3 days of vibration alone. Sinusoidal stimulation alone did not affect the cell responses. No differences due to loading were observed in alkaline phosphatase activity and in mRNA levels of type I collagen, osteopontin, connexin 43, MMPs-1A, -3, -13. These results suggest that osteoblasts are more sensitive to low amplitude, broad frequency strain, and this kind of strain could sensitize osteoblasts to high amplitude, low frequency strain. This suggestion implies a potential contribution of stochastic resonance to the mechanical sensitivity of osteoblasts.

AB - Bone is subjected in vivo to both high amplitude, low frequency strain, incurred by locomotion, and to low amplitude, broad frequency strain. The biological effects of low amplitude, broad frequency strain are poorly understood. To evaluate the effects of low amplitude strains ranging in frequency from 0 to 50Hz on osteoblastic function, we seeded MC3T3-E1 cells into collagen gels and applied the following loading protocols for 3min per day for either 3 or 7 days: (1) sinusoidal strain at 3Hz, with 0-3000μstrain peak-to-peak followed by 0.33s resting time, (2) "broad frequency vibration" of low amplitude strain (standard deviation of 300μstrain) including frequency components from 0 to 50Hz, and (3) sinusoidal strain combined with broad frequency vibration (S+V). The cells were harvested on day 4 or 8. We found that the S+V stimulation significantly repressed cell proliferation by day 8. Osteocalcin mRNA was up-regulated 2.6-fold after 7 days of S+V stimulation, and MMP-9 mRNA was elevated 1.3-fold after 3 days of vibration alone. Sinusoidal stimulation alone did not affect the cell responses. No differences due to loading were observed in alkaline phosphatase activity and in mRNA levels of type I collagen, osteopontin, connexin 43, MMPs-1A, -3, -13. These results suggest that osteoblasts are more sensitive to low amplitude, broad frequency strain, and this kind of strain could sensitize osteoblasts to high amplitude, low frequency strain. This suggestion implies a potential contribution of stochastic resonance to the mechanical sensitivity of osteoblasts.

KW - Biomechanics

KW - Bone

KW - Mechanical vibration

KW - Osteoblasts

KW - Osteoporosis

UR - http://www.scopus.com/inward/record.url?scp=2242449754&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2242449754&partnerID=8YFLogxK

U2 - 10.1016/S0021-9290(02)00245-2

DO - 10.1016/S0021-9290(02)00245-2

M3 - Article

VL - 36

SP - 73

EP - 80

JO - Journal of Biomechanics

JF - Journal of Biomechanics

SN - 0021-9290

IS - 1

ER -