Effects of Calendula officinalis on human gingival fibroblasts

Pragtipal Saini, Nouf Al-Shibani, Jun Sun, Weiping Zhang, Fengyu Song, Karen S. Gregson, L. Jack Windsor

Research output: Contribution to journalArticle

17 Scopus citations


Background: Calendula officinalis is commonly called the marigold. It is a staple topical remedy in homeopathic medicine. It is rich in quercetin, carotenoids, lutein, lycopene, rutin, ubiquinone, xanthophylls, and other anti-oxidants. It has anti-inflammatory properties. Quercetin, one of the active components in . Calendula, has been shown to inhibit recombinant human matrix metalloproteinase (MMP) activity and decrease the expression of tumor necrosis factor-α, interleukin-1β (IL), IL-6 and IL-8 in phorbol 12-myristate 13-acetate and calcium ionophore-stimulated human mast cells. Objectives: To examine the effects of . Calendula on human gingival fibroblast (HGF) mediated collagen degradation and MMP activity. Material and methods: Lactate dehydrogenate assays were performed to determine the non-toxic concentrations of . Calendula, doxycycline and quercetin. Cell-mediated collagen degradation assays were performed to examine the inhibitory effect on cell-mediated collagen degradation. Gelatin zymography was performed to examine their effects on MMP-2 activity. The experiments were repeated three times and ANOVA used for statistical analyses. Results: Calendula at 2-3% completely inhibited the MMP-2 activity in the zymograms. Doxycycline inhibited HGF-mediated collagen degradation at 0.005, 0.01, 0.02 and 0.05%, and MMP-2 activity completely at 0.05%. Quercetin inhibited HGF-mediated collagen degradation at 0.005, 0.01 and 0.02%, and MMP-2 activity in a dose-dependent manner. . Calendula inhibited HGF-mediated collagen degradation and MMP-2 activity more than the same correlated concentration of pure quercetin. Conclusion: Calendula inhibits HGF-mediated collagen degradation and MMP-2 activity more than the corresponding concentration of quercetin. This may be attributed to additional components in . Calendula other than quercetin.

Original languageEnglish (US)
Pages (from-to)92-98
Number of pages7
Issue number2
StatePublished - Apr 1 2012



  • Calendula
  • Doxycycline
  • Gingival fibroblasts
  • Matrix Metalloproteinases
  • Periodontal disease

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Saini, P., Al-Shibani, N., Sun, J., Zhang, W., Song, F., Gregson, K. S., & Windsor, L. J. (2012). Effects of Calendula officinalis on human gingival fibroblasts. Homeopathy, 101(2), 92-98. https://doi.org/10.1016/j.homp.2012.02.003