Effects of CC, CXC, C, and CX3C chemokines on proliferation of myeloid progenitor cells, and insights into SDF-1-induced chemotaxis of progenitors

Hal Broxmeyer, Chang H. Kim, Scott H. Cooper, Giao Hangoc, Robert Hromas, Louis Pelus

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Chemokines have been implicated In the regulation of stem/progenitor cell proliferation and movement. The purpose of the present study was to assess a number of new chemokines for suppressive activity and to delve further into SDF-1-mediated chemotaxis of progenitor cells. This report extends the list of chemokines that have suppressive activity against immature subsets of myeloid progenitors stimulated to proliferate by multiple growth factors to include: MCP-4/CKβ-10, MIP-4/CKβ-7, I-309, TECK, GCP-2, MIG and lymphotactin. The suppressive activity of a number of other chemokines was confirmed. Additionally, pretreatment of the active chemokines with an acetylnitrile solution enhanced specific activity of a number of these chemokines. The new chemokines found to be lacking suppressive activity include: MCP-2, MCP-3, eotaxin-1, MCIF/HCC-1/CKβ-1, TARC, MDC, MPIF-2/eotaxin-2/CKβ-6, SDF-1 and fractalkine/neurotactin. Overall, 19 chemokines, crossing the CC, CXC, and C subgroups, have now been found to be myelosuppressive, and 14 chemokines crossing the CC, CXC and CX3C subgroups have been found to lack myelosuppressive activity under the culture conditions of our assays. Because of the redundancy in chemokine/chemokine receptor interactions, it is not yet clear through which chemokine receptors many of these chemokines signal to elicit suppressive activities. It was also found that SDF-1-induced chemotaxis of progenitors can occur in the presence of fibronectin (FN) and extracellular matrix components and that FN effects involve activation of β1-, and possibly α4-, integrins.

Original languageEnglish
Pages (from-to)142-163
Number of pages22
JournalAnnals of the New York Academy of Sciences
Volume872
DOIs
StatePublished - 1999

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CX3C Chemokines
C Chemokines
Myeloid Progenitor Cells
CXC Chemokines
CC Chemokines
Chemotaxis
Chemokines
Chemokine CX3CL1
Stem Cells
Chemokine Receptors
Fibronectins
Chemokine CCL24
Chemokine CCL11
Cells
Cell proliferation
Stem cells
Integrins
Cell Movement
Extracellular Matrix
Redundancy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effects of CC, CXC, C, and CX3C chemokines on proliferation of myeloid progenitor cells, and insights into SDF-1-induced chemotaxis of progenitors. / Broxmeyer, Hal; Kim, Chang H.; Cooper, Scott H.; Hangoc, Giao; Hromas, Robert; Pelus, Louis.

In: Annals of the New York Academy of Sciences, Vol. 872, 1999, p. 142-163.

Research output: Contribution to journalArticle

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