Effects of CD34+ selection and T cell immunodepletion on cord blood hematopoietic progenitors

Relevance to stem cell transplantation

J. Laver, Christie Orschell, A. Abdel-Mageed, A. Gee, C. Lee, C. Turner, Edward Srour, M. Abboud

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cord blood (CB) has been used recently for stem cell transplantation. We have investigated two different approaches to deplete CB samples of T cells capable of mounting graft-vs.-host disease (GVHD). The methods used were selection of CD34+ cells using avidin-biotin columns (CellPro) and T cell immunodepletion with T10B9 monoclonal antibody (mAb) plus complement. Using the avidin-biotin columns, 10.3% of the original CD34+ cells were recovered. Although this technique yielded a population containing 60 ± 5.5% CD34+ cells, about 1 log of CFU-GM progenitors were lost. In contrast, after the T10B9 mAb and complement immunodepletion, 75 ± 19% and 62 ± 7% of the CD34+ cells and CFU-GM were recovered, respectively. T cell depletion was 3.6 logs using the CellPro columns and 2.2 logs after immunodepletion. To investigate whether cell losses following T cell depletion could be overcome by ex vivo expansion, cells were cultured in the presence of recombinant human interleukin-3 (rhIL-3) and recombinant human c-kit ligand (stem cell factor [rhSCF]) for 7 days. There were 14- and six-fold expansions in the number of progenitors recovered after CellPro and immunodepletion, respectively. To assess the engraftment potential of expanded cells, we used a murine transplantation model in which the presence of human cells was identified by the anti-CD45 mAb. Cells expanded in vitro engrafted in irradiated BNXid mice as efficiently as nonexpanded cells, suggesting that expansion did not affect their transplantability. This study shows that both techniques resulted in significant T cell depletion of CB. Furthermore, in vitro expansion could overcome cell losses sustained during the separation techniques without impairing the engraftment potential of the expanded cells.

Original languageEnglish (US)
Pages (from-to)1492-1496
Number of pages5
JournalExperimental Hematology
Volume23
Issue number14
StatePublished - 1995
Externally publishedYes

Fingerprint

Stem Cell Transplantation
Fetal Blood
T-Lymphocytes
Granulocyte-Macrophage Progenitor Cells
Stem Cell Factor
Avidin
Monoclonal Antibodies
Biotin
Interleukin-3
Cultured Cells
Transplantation
Transplants

Keywords

  • CD34
  • Cord blood
  • Immunodepletion
  • Stem cells

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Effects of CD34+ selection and T cell immunodepletion on cord blood hematopoietic progenitors : Relevance to stem cell transplantation. / Laver, J.; Orschell, Christie; Abdel-Mageed, A.; Gee, A.; Lee, C.; Turner, C.; Srour, Edward; Abboud, M.

In: Experimental Hematology, Vol. 23, No. 14, 1995, p. 1492-1496.

Research output: Contribution to journalArticle

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abstract = "Cord blood (CB) has been used recently for stem cell transplantation. We have investigated two different approaches to deplete CB samples of T cells capable of mounting graft-vs.-host disease (GVHD). The methods used were selection of CD34+ cells using avidin-biotin columns (CellPro) and T cell immunodepletion with T10B9 monoclonal antibody (mAb) plus complement. Using the avidin-biotin columns, 10.3{\%} of the original CD34+ cells were recovered. Although this technique yielded a population containing 60 ± 5.5{\%} CD34+ cells, about 1 log of CFU-GM progenitors were lost. In contrast, after the T10B9 mAb and complement immunodepletion, 75 ± 19{\%} and 62 ± 7{\%} of the CD34+ cells and CFU-GM were recovered, respectively. T cell depletion was 3.6 logs using the CellPro columns and 2.2 logs after immunodepletion. To investigate whether cell losses following T cell depletion could be overcome by ex vivo expansion, cells were cultured in the presence of recombinant human interleukin-3 (rhIL-3) and recombinant human c-kit ligand (stem cell factor [rhSCF]) for 7 days. There were 14- and six-fold expansions in the number of progenitors recovered after CellPro and immunodepletion, respectively. To assess the engraftment potential of expanded cells, we used a murine transplantation model in which the presence of human cells was identified by the anti-CD45 mAb. Cells expanded in vitro engrafted in irradiated BNXid mice as efficiently as nonexpanded cells, suggesting that expansion did not affect their transplantability. This study shows that both techniques resulted in significant T cell depletion of CB. Furthermore, in vitro expansion could overcome cell losses sustained during the separation techniques without impairing the engraftment potential of the expanded cells.",
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