Effects of chemopreventive and antitelomerase agents on the spontaneous immortalization of breast epithelial cells

Brittney-Shea Herbert, Angelique C. Wright, Christina M. Passons, Woodring E. Wright, Iqbal Unnisa Ali, Levy Kopelovich, Jerry W. Shay

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: Activation of telomerase is an early event in the development of breast and other cancers that may lead to cell immortalization, a critical and rate-limiting step in cancer progression. Breast epithelial cells from women with Li-Fraumeni syndrome (LFS) immortalize spontaneously and reproducibly in culture. We, therefore, tested whether immortalization of these cells could be prevented by treating them with chemopreventive agents and by inhibiting telomerase activity. Methods: Noncancerous, preimmortal breast epithelial cells derived from a patient with LFS were treated for 3 months with nontoxic concentrations of the chemopreventive agents oltipraz, difluoromethylornithine, tamoxifen, and retinoic acid or with two different telomerase inhibitors. The frequency of spontaneous immortalization of LFS-derived cells was estimated by an approach based on fluctuation analyses. Statistical analyses were two-sided. Results: The frequency of spontaneous immortalization events of LFS-derived breast epithelial cells was reduced by long-term treatment with retinoic acid (P

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalJournal of the National Cancer Institute
Volume93
Issue number1
StatePublished - Jan 3 2001
Externally publishedYes

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Li-Fraumeni Syndrome
Telomerase
Breast
Epithelial Cells
Tretinoin
Eflornithine
Breast Neoplasms
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Herbert, B-S., Wright, A. C., Passons, C. M., Wright, W. E., Ali, I. U., Kopelovich, L., & Shay, J. W. (2001). Effects of chemopreventive and antitelomerase agents on the spontaneous immortalization of breast epithelial cells. Journal of the National Cancer Institute, 93(1), 39-45.

Effects of chemopreventive and antitelomerase agents on the spontaneous immortalization of breast epithelial cells. / Herbert, Brittney-Shea; Wright, Angelique C.; Passons, Christina M.; Wright, Woodring E.; Ali, Iqbal Unnisa; Kopelovich, Levy; Shay, Jerry W.

In: Journal of the National Cancer Institute, Vol. 93, No. 1, 03.01.2001, p. 39-45.

Research output: Contribution to journalArticle

Herbert, B-S, Wright, AC, Passons, CM, Wright, WE, Ali, IU, Kopelovich, L & Shay, JW 2001, 'Effects of chemopreventive and antitelomerase agents on the spontaneous immortalization of breast epithelial cells', Journal of the National Cancer Institute, vol. 93, no. 1, pp. 39-45.
Herbert, Brittney-Shea ; Wright, Angelique C. ; Passons, Christina M. ; Wright, Woodring E. ; Ali, Iqbal Unnisa ; Kopelovich, Levy ; Shay, Jerry W. / Effects of chemopreventive and antitelomerase agents on the spontaneous immortalization of breast epithelial cells. In: Journal of the National Cancer Institute. 2001 ; Vol. 93, No. 1. pp. 39-45.
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AU - Ali, Iqbal Unnisa

AU - Kopelovich, Levy

AU - Shay, Jerry W.

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