Effects of Ciprofloxacin-containing Scaffolds on Enterococcus faecalis Biofilms

Maria T P Albuquerque, Marcia C. Valera, Camila S. Moreira, Eduardo Bresciani, Renata M. De Melo, Marco C. Bottino

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Introduction: Antibiotic-containing polymer-based nanofibers (hereafter referred to as scaffolds) have demonstrated great potential for their use in regenerative endodontics from both an antimicrobial and cytocompatibility perspective. This study sought to evaluate in vitro the effects of ciprofloxacin (CIP)-containing polymer scaffolds against Enterococcus faecalis biofilms. Methods: Human mandibular incisors were longitudinally sectioned to prepare radicular dentin specimens. Sterile dentin specimens were distributed in 24-well plates and inoculated with E. faecalis for biofilm formation. Infected dentin specimens were exposed to 3 groups of scaffolds, namely polydioxanone (PDS) (control), PDS + 5 wt% CIP, and PDS + 25 wt% CIP for 2 days. Colony-forming units (CFU/mL) (n = 10) and scanning electron microscopy (SEM) (n = 2) were performed to quantitatively and qualitatively assess the antimicrobial effectiveness, respectively. Results: PDS scaffold containing CIP at 25 wt% showed maximum bacteria elimination with no microbial growth, differing statistically (P

Original languageEnglish (US)
Pages (from-to)710-714
Number of pages5
JournalJournal of Endodontics
Volume41
Issue number5
DOIs
StatePublished - May 1 2015
Externally publishedYes

Fingerprint

Enterococcus faecalis
Ciprofloxacin
Biofilms
Dentin
Polymers
Polydioxanone
Nanofibers
Endodontics
Incisor
Electron Scanning Microscopy
Stem Cells
Anti-Bacterial Agents
Bacteria
Growth

Keywords

  • Antibiotic
  • bacteria
  • disinfection
  • electrospinning
  • nanofibers
  • pulp
  • regeneration
  • root canal
  • scaffold
  • stem cells

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Albuquerque, M. T. P., Valera, M. C., Moreira, C. S., Bresciani, E., De Melo, R. M., & Bottino, M. C. (2015). Effects of Ciprofloxacin-containing Scaffolds on Enterococcus faecalis Biofilms. Journal of Endodontics, 41(5), 710-714. https://doi.org/10.1016/j.joen.2014.12.025

Effects of Ciprofloxacin-containing Scaffolds on Enterococcus faecalis Biofilms. / Albuquerque, Maria T P; Valera, Marcia C.; Moreira, Camila S.; Bresciani, Eduardo; De Melo, Renata M.; Bottino, Marco C.

In: Journal of Endodontics, Vol. 41, No. 5, 01.05.2015, p. 710-714.

Research output: Contribution to journalArticle

Albuquerque, MTP, Valera, MC, Moreira, CS, Bresciani, E, De Melo, RM & Bottino, MC 2015, 'Effects of Ciprofloxacin-containing Scaffolds on Enterococcus faecalis Biofilms', Journal of Endodontics, vol. 41, no. 5, pp. 710-714. https://doi.org/10.1016/j.joen.2014.12.025
Albuquerque MTP, Valera MC, Moreira CS, Bresciani E, De Melo RM, Bottino MC. Effects of Ciprofloxacin-containing Scaffolds on Enterococcus faecalis Biofilms. Journal of Endodontics. 2015 May 1;41(5):710-714. https://doi.org/10.1016/j.joen.2014.12.025
Albuquerque, Maria T P ; Valera, Marcia C. ; Moreira, Camila S. ; Bresciani, Eduardo ; De Melo, Renata M. ; Bottino, Marco C. / Effects of Ciprofloxacin-containing Scaffolds on Enterococcus faecalis Biofilms. In: Journal of Endodontics. 2015 ; Vol. 41, No. 5. pp. 710-714.
@article{e5d234119dce47b48239e1b2f3a99233,
title = "Effects of Ciprofloxacin-containing Scaffolds on Enterococcus faecalis Biofilms",
abstract = "Introduction: Antibiotic-containing polymer-based nanofibers (hereafter referred to as scaffolds) have demonstrated great potential for their use in regenerative endodontics from both an antimicrobial and cytocompatibility perspective. This study sought to evaluate in vitro the effects of ciprofloxacin (CIP)-containing polymer scaffolds against Enterococcus faecalis biofilms. Methods: Human mandibular incisors were longitudinally sectioned to prepare radicular dentin specimens. Sterile dentin specimens were distributed in 24-well plates and inoculated with E. faecalis for biofilm formation. Infected dentin specimens were exposed to 3 groups of scaffolds, namely polydioxanone (PDS) (control), PDS + 5 wt{\%} CIP, and PDS + 25 wt{\%} CIP for 2 days. Colony-forming units (CFU/mL) (n = 10) and scanning electron microscopy (SEM) (n = 2) were performed to quantitatively and qualitatively assess the antimicrobial effectiveness, respectively. Results: PDS scaffold containing CIP at 25 wt{\%} showed maximum bacteria elimination with no microbial growth, differing statistically (P",
keywords = "Antibiotic, bacteria, disinfection, electrospinning, nanofibers, pulp, regeneration, root canal, scaffold, stem cells",
author = "Albuquerque, {Maria T P} and Valera, {Marcia C.} and Moreira, {Camila S.} and Eduardo Bresciani and {De Melo}, {Renata M.} and Bottino, {Marco C.}",
year = "2015",
month = "5",
day = "1",
doi = "10.1016/j.joen.2014.12.025",
language = "English (US)",
volume = "41",
pages = "710--714",
journal = "Journal of Endodontics",
issn = "0099-2399",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Effects of Ciprofloxacin-containing Scaffolds on Enterococcus faecalis Biofilms

AU - Albuquerque, Maria T P

AU - Valera, Marcia C.

AU - Moreira, Camila S.

AU - Bresciani, Eduardo

AU - De Melo, Renata M.

AU - Bottino, Marco C.

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Introduction: Antibiotic-containing polymer-based nanofibers (hereafter referred to as scaffolds) have demonstrated great potential for their use in regenerative endodontics from both an antimicrobial and cytocompatibility perspective. This study sought to evaluate in vitro the effects of ciprofloxacin (CIP)-containing polymer scaffolds against Enterococcus faecalis biofilms. Methods: Human mandibular incisors were longitudinally sectioned to prepare radicular dentin specimens. Sterile dentin specimens were distributed in 24-well plates and inoculated with E. faecalis for biofilm formation. Infected dentin specimens were exposed to 3 groups of scaffolds, namely polydioxanone (PDS) (control), PDS + 5 wt% CIP, and PDS + 25 wt% CIP for 2 days. Colony-forming units (CFU/mL) (n = 10) and scanning electron microscopy (SEM) (n = 2) were performed to quantitatively and qualitatively assess the antimicrobial effectiveness, respectively. Results: PDS scaffold containing CIP at 25 wt% showed maximum bacteria elimination with no microbial growth, differing statistically (P

AB - Introduction: Antibiotic-containing polymer-based nanofibers (hereafter referred to as scaffolds) have demonstrated great potential for their use in regenerative endodontics from both an antimicrobial and cytocompatibility perspective. This study sought to evaluate in vitro the effects of ciprofloxacin (CIP)-containing polymer scaffolds against Enterococcus faecalis biofilms. Methods: Human mandibular incisors were longitudinally sectioned to prepare radicular dentin specimens. Sterile dentin specimens were distributed in 24-well plates and inoculated with E. faecalis for biofilm formation. Infected dentin specimens were exposed to 3 groups of scaffolds, namely polydioxanone (PDS) (control), PDS + 5 wt% CIP, and PDS + 25 wt% CIP for 2 days. Colony-forming units (CFU/mL) (n = 10) and scanning electron microscopy (SEM) (n = 2) were performed to quantitatively and qualitatively assess the antimicrobial effectiveness, respectively. Results: PDS scaffold containing CIP at 25 wt% showed maximum bacteria elimination with no microbial growth, differing statistically (P

KW - Antibiotic

KW - bacteria

KW - disinfection

KW - electrospinning

KW - nanofibers

KW - pulp

KW - regeneration

KW - root canal

KW - scaffold

KW - stem cells

UR - http://www.scopus.com/inward/record.url?scp=84929028748&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929028748&partnerID=8YFLogxK

U2 - 10.1016/j.joen.2014.12.025

DO - 10.1016/j.joen.2014.12.025

M3 - Article

VL - 41

SP - 710

EP - 714

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 5

ER -