Effects of cytochalasin B and colcemide on myogenic cultures

H. Holtzer, James Croop, S. Dienstman

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Muscle cultures treated with cytochalasin B yield mono and oligonucleated cells of two kinds: (i) arborized, replicating precursor myogenic cells and fibroblasts; and (ii) round, post mitotic, terminally differentiating myoblasts and myotubes. The arborized cells do not bind fluorescein labeled antibody against myosin, do not contract rhythmically, and do not display hexagonally stacked thick and thin filaments. The round, mononucleated myoblasts and round, oligonucleated myotubes bind the fluorescein labeled antibody against myosin, contract rhythmically, and display clusters of hexagonally stacked thick and thin filaments. When cytochalasin B is removed and replaced by colcemide, the arborized cells, but not the post mitotic muscle cells, acquire a radial symmetry and are induced to assemble massive meandering cables that may occupy over 25% of the cell volume. These tortuous cables are positively birefringent and consist exclusively of enormous numbers of 100 Å intermediate sized filaments.

Original languageEnglish (US)
Pages (from-to)513-517
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number2
StatePublished - 1975
Externally publishedYes

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Demecolcine
Cytochalasin B
Myoblasts
Skeletal Muscle Fibers
Myosins
Fluorescein
Intermediate Filaments
Antibodies
Cell Size
Muscle Cells
Fibroblasts
Muscles

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Effects of cytochalasin B and colcemide on myogenic cultures. / Holtzer, H.; Croop, James; Dienstman, S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, No. 2, 1975, p. 513-517.

Research output: Contribution to journalArticle

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