Effects of cytochalasin B and colcemide on myogenic cultures

H. Holtzer, J. Croop, S. Dienstman

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Muscle cultures treated with cytochalasin B yield mono and oligonucleated cells of two kinds: (i) arborized, replicating precursor myogenic cells and fibroblasts; and (ii) round, post mitotic, terminally differentiating myoblasts and myotubes. The arborized cells do not bind fluorescein labeled antibody against myosin, do not contract rhythmically, and do not display hexagonally stacked thick and thin filaments. The round, mononucleated myoblasts and round, oligonucleated myotubes bind the fluorescein labeled antibody against myosin, contract rhythmically, and display clusters of hexagonally stacked thick and thin filaments. When cytochalasin B is removed and replaced by colcemide, the arborized cells, but not the post mitotic muscle cells, acquire a radial symmetry and are induced to assemble massive meandering cables that may occupy over 25% of the cell volume. These tortuous cables are positively birefringent and consist exclusively of enormous numbers of 100 Å intermediate sized filaments.

Original languageEnglish (US)
Pages (from-to)513-517
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number2
DOIs
StatePublished - Jan 1 1975
Externally publishedYes

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