Effects of dietary manganese deficiency on rat pancreatic amylase mRNA levels

S. C. Chang, P. M. Brannon, Murray Korc

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Manganese deficiency is associated with increased pancreatic amylasae activity in rats. The present study investigated whether this increase in amylase activity is associated with increased pancreatic amylase messenger RNA (mRNA) levels. Weanling rats were fed a high carbohydrate diet containing either 40 μg Mn/g (control) or 0.5 μg Mn/g (deficient) for 4 to 8 wk. Mn deficiency was confirmed by determining hepatic Mn content, which was significantly lower in Mn-deficient rats than in controls. Pancreatic RNA from both groups of rats was hybridized with 32P-labeled complementary DNAs for amylase and trypsinogen. Amylase mRNA levels were increased in rats fed Mn-deficient diets for both 4 wk (200%) and 8 wk (250%) when compared with respective control levels. In contrast, Mn deficiency was not associated with alterations in trypsinogen mRNA levels. Serum levels of insulin and corticosterone, hormones known to increase pancreatic amylase mRNA, were not affected by Mn deficiency. These observations suggest that Mn may participate in the regulation of amylase gene expression in a manner that is independent of insulin and corticosterone.

Original languageEnglish (US)
Pages (from-to)1228-1234
Number of pages7
JournalJournal of Nutrition
Volume120
Issue number10
StatePublished - 1990
Externally publishedYes

Fingerprint

Amylases
Manganese
messenger RNA
amylases
manganese
Messenger RNA
rats
trypsinogen
Trypsinogen
corticosterone
Corticosterone
insulin
Insulin
Diet
high carbohydrate diet
complementary DNA
Gene Expression Regulation
weanlings
Complementary DNA
Carbohydrates

Keywords

  • amylase mRNA
  • manganese
  • rats
  • trypsinogen mRNA

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Chang, S. C., Brannon, P. M., & Korc, M. (1990). Effects of dietary manganese deficiency on rat pancreatic amylase mRNA levels. Journal of Nutrition, 120(10), 1228-1234.

Effects of dietary manganese deficiency on rat pancreatic amylase mRNA levels. / Chang, S. C.; Brannon, P. M.; Korc, Murray.

In: Journal of Nutrition, Vol. 120, No. 10, 1990, p. 1228-1234.

Research output: Contribution to journalArticle

Chang, SC, Brannon, PM & Korc, M 1990, 'Effects of dietary manganese deficiency on rat pancreatic amylase mRNA levels', Journal of Nutrition, vol. 120, no. 10, pp. 1228-1234.
Chang, S. C. ; Brannon, P. M. ; Korc, Murray. / Effects of dietary manganese deficiency on rat pancreatic amylase mRNA levels. In: Journal of Nutrition. 1990 ; Vol. 120, No. 10. pp. 1228-1234.
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