Effects of doxycycline on human prostate cancer cells in vitro

Rose S. Fife, George W. Sledge, Bruce J. Roth, Carmen Proctor

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Prostate cancer is the most common form of cancer in older men and the major cause of death from prostate cancer is metastatic disease. The matrix metalloproteinases (MMPs) play a significant role in the growth, invasion and metastasis of many tumors, including those of the prostate. We previously demonstrated that doxycycline, a synthetic tetracycline, inhibits MMPs and cell proliferation and induces apoptosis in several cancer cell lines. We also demonstrated that in an in vivo model of metastatic breast cancer in athymic mice doxycycline inhibits tumor size and regrowth after resection. In the present study, gelatinolytic activity in the human prostate cancer cell line, LNCaP, was suppressed and significant inhibition of cell growth occurred after exposure to 5 or 10 μg/ml of doxycycline, while cell growth was normal in untreated cells. Radioisotope incorporation into proteins was reduced by doxycycline. DNA fragmentation, consistent with apoptosis, was demonstrated in cells treated with doxycycline. These data suggest that doxycycline may have potential utility in the management of prostate cancer.

Original languageEnglish (US)
Pages (from-to)37-41
Number of pages5
JournalCancer Letters
Volume127
Issue number1-2
DOIs
StatePublished - May 15 1998

Keywords

  • Doxycycline
  • Human prostate cancer cells
  • In vitro

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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