Effects of endothelin-1 and homologous trout endothelin on cardiovascular function in rainbow trout

Todd M. Hoagland, Leroy Weaver, J. Michael Conlon, Yugi Wang, Kenneth R. Olson

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The cardiovascular effects of endothelin (ET)-1 and the recently sequenced homologous trout ET were examined in unanesthetized trout, and vascular capacitance curves were constructed to evaluate the responsiveness of the venous system to ET-1. A bolus dose of 667 pmol/kg ET-1 doubled ventral aortic pressure; produced a triphasic pressor-depressor-pressor response in dorsal aortic pressure (P(DA)); increased central venous pressure, gill resistance, and systemic resistance; and decreased cardiac output, heart rate, and stroke volume. TheSe responses were dose dependent. Bolus injection of trout ET (333 or 1,000 pmol/kg) produced essentially identical, dose-dependent cardiovascular responses as ET-1. Dorsal aortic infusion of 1 and 3 pmol·kg-1·min-1 ET-1 and central venous infusion into the ductus Cuvier of 0.3 and 1 pmol·kg-1. min-1 produced similar dose-dependent cardiovascular responses, although the increase in P(DA) became monophasic. The heightened sensitivity to central venous infusion was presumably due to the more immediate exposure of the branchial vasculature to the peptide. Infusion of 1 pmol·kg-1·min-1 ET-1 decreased vascular compliance but had no effect on unstressed blood volume. These results show that ETs affect a variety of cardiovascular functions in trout and that branchial vascular resistance and venous compliance are especially sensitive. The multiplicity of effectors stimulated by ET suggests that this peptide was extensively integrated into cardiovascular function early on in vertebrate phylogeny.

Original languageEnglish (US)
Pages (from-to)R460-R468
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume278
Issue number2 47-2
DOIs
StatePublished - Feb 2000

Keywords

  • Fish
  • Mean circulatory filling pressure
  • Unstressed blood volume
  • Vascular capacitance

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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