Effects of endothelin-1 and homologous trout endothelin on cardiovascular function in rainbow trout

Todd M. Hoagland, Leroy Weaver, J. Michael Conlon, Yugi Wang, Kenneth Olson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The cardiovascular effects of endothelin (ET)-1 and the recently sequenced homologous trout ET were examined in unanesthetized trout, and vascular capacitance curves were constructed to evaluate the responsiveness of the venous system to ET-1. A bolus dose of 667 pmol/kg ET-1 doubled ventral aortic pressure; produced a triphasic pressor-depressor-pressor response in dorsal aortic pressure (P(DA)); increased central venous pressure, gill resistance, and systemic resistance; and decreased cardiac output, heart rate, and stroke volume. TheSe responses were dose dependent. Bolus injection of trout ET (333 or 1,000 pmol/kg) produced essentially identical, dose-dependent cardiovascular responses as ET-1. Dorsal aortic infusion of 1 and 3 pmol·kg-1·min-1 ET-1 and central venous infusion into the ductus Cuvier of 0.3 and 1 pmol·kg-1. min-1 produced similar dose-dependent cardiovascular responses, although the increase in P(DA) became monophasic. The heightened sensitivity to central venous infusion was presumably due to the more immediate exposure of the branchial vasculature to the peptide. Infusion of 1 pmol·kg-1·min-1 ET-1 decreased vascular compliance but had no effect on unstressed blood volume. These results show that ETs affect a variety of cardiovascular functions in trout and that branchial vascular resistance and venous compliance are especially sensitive. The multiplicity of effectors stimulated by ET suggests that this peptide was extensively integrated into cardiovascular function early on in vertebrate phylogeny.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume278
Issue number2 47-2
StatePublished - Feb 2000

Fingerprint

Trout
Oncorhynchus mykiss
Endothelins
Endothelin-1
Compliance
Vascular Capacitance
Arterial Pressure
Cardiac Volume
Peptides
Central Venous Pressure
Phylogeny
Blood Volume
Cardiac Output
Vascular Resistance
Stroke Volume
Blood Vessels
Vertebrates
Heart Rate
Injections

Keywords

  • Fish
  • Mean circulatory filling pressure
  • Unstressed blood volume
  • Vascular capacitance

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Effects of endothelin-1 and homologous trout endothelin on cardiovascular function in rainbow trout. / Hoagland, Todd M.; Weaver, Leroy; Conlon, J. Michael; Wang, Yugi; Olson, Kenneth.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 278, No. 2 47-2, 02.2000.

Research output: Contribution to journalArticle

@article{8761e92fe21e41d9b5e28517f4ee3c07,
title = "Effects of endothelin-1 and homologous trout endothelin on cardiovascular function in rainbow trout",
abstract = "The cardiovascular effects of endothelin (ET)-1 and the recently sequenced homologous trout ET were examined in unanesthetized trout, and vascular capacitance curves were constructed to evaluate the responsiveness of the venous system to ET-1. A bolus dose of 667 pmol/kg ET-1 doubled ventral aortic pressure; produced a triphasic pressor-depressor-pressor response in dorsal aortic pressure (P(DA)); increased central venous pressure, gill resistance, and systemic resistance; and decreased cardiac output, heart rate, and stroke volume. TheSe responses were dose dependent. Bolus injection of trout ET (333 or 1,000 pmol/kg) produced essentially identical, dose-dependent cardiovascular responses as ET-1. Dorsal aortic infusion of 1 and 3 pmol·kg-1·min-1 ET-1 and central venous infusion into the ductus Cuvier of 0.3 and 1 pmol·kg-1. min-1 produced similar dose-dependent cardiovascular responses, although the increase in P(DA) became monophasic. The heightened sensitivity to central venous infusion was presumably due to the more immediate exposure of the branchial vasculature to the peptide. Infusion of 1 pmol·kg-1·min-1 ET-1 decreased vascular compliance but had no effect on unstressed blood volume. These results show that ETs affect a variety of cardiovascular functions in trout and that branchial vascular resistance and venous compliance are especially sensitive. The multiplicity of effectors stimulated by ET suggests that this peptide was extensively integrated into cardiovascular function early on in vertebrate phylogeny.",
keywords = "Fish, Mean circulatory filling pressure, Unstressed blood volume, Vascular capacitance",
author = "Hoagland, {Todd M.} and Leroy Weaver and Conlon, {J. Michael} and Yugi Wang and Kenneth Olson",
year = "2000",
month = "2",
language = "English",
volume = "278",
journal = "American Journal of Physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "2 47-2",

}

TY - JOUR

T1 - Effects of endothelin-1 and homologous trout endothelin on cardiovascular function in rainbow trout

AU - Hoagland, Todd M.

AU - Weaver, Leroy

AU - Conlon, J. Michael

AU - Wang, Yugi

AU - Olson, Kenneth

PY - 2000/2

Y1 - 2000/2

N2 - The cardiovascular effects of endothelin (ET)-1 and the recently sequenced homologous trout ET were examined in unanesthetized trout, and vascular capacitance curves were constructed to evaluate the responsiveness of the venous system to ET-1. A bolus dose of 667 pmol/kg ET-1 doubled ventral aortic pressure; produced a triphasic pressor-depressor-pressor response in dorsal aortic pressure (P(DA)); increased central venous pressure, gill resistance, and systemic resistance; and decreased cardiac output, heart rate, and stroke volume. TheSe responses were dose dependent. Bolus injection of trout ET (333 or 1,000 pmol/kg) produced essentially identical, dose-dependent cardiovascular responses as ET-1. Dorsal aortic infusion of 1 and 3 pmol·kg-1·min-1 ET-1 and central venous infusion into the ductus Cuvier of 0.3 and 1 pmol·kg-1. min-1 produced similar dose-dependent cardiovascular responses, although the increase in P(DA) became monophasic. The heightened sensitivity to central venous infusion was presumably due to the more immediate exposure of the branchial vasculature to the peptide. Infusion of 1 pmol·kg-1·min-1 ET-1 decreased vascular compliance but had no effect on unstressed blood volume. These results show that ETs affect a variety of cardiovascular functions in trout and that branchial vascular resistance and venous compliance are especially sensitive. The multiplicity of effectors stimulated by ET suggests that this peptide was extensively integrated into cardiovascular function early on in vertebrate phylogeny.

AB - The cardiovascular effects of endothelin (ET)-1 and the recently sequenced homologous trout ET were examined in unanesthetized trout, and vascular capacitance curves were constructed to evaluate the responsiveness of the venous system to ET-1. A bolus dose of 667 pmol/kg ET-1 doubled ventral aortic pressure; produced a triphasic pressor-depressor-pressor response in dorsal aortic pressure (P(DA)); increased central venous pressure, gill resistance, and systemic resistance; and decreased cardiac output, heart rate, and stroke volume. TheSe responses were dose dependent. Bolus injection of trout ET (333 or 1,000 pmol/kg) produced essentially identical, dose-dependent cardiovascular responses as ET-1. Dorsal aortic infusion of 1 and 3 pmol·kg-1·min-1 ET-1 and central venous infusion into the ductus Cuvier of 0.3 and 1 pmol·kg-1. min-1 produced similar dose-dependent cardiovascular responses, although the increase in P(DA) became monophasic. The heightened sensitivity to central venous infusion was presumably due to the more immediate exposure of the branchial vasculature to the peptide. Infusion of 1 pmol·kg-1·min-1 ET-1 decreased vascular compliance but had no effect on unstressed blood volume. These results show that ETs affect a variety of cardiovascular functions in trout and that branchial vascular resistance and venous compliance are especially sensitive. The multiplicity of effectors stimulated by ET suggests that this peptide was extensively integrated into cardiovascular function early on in vertebrate phylogeny.

KW - Fish

KW - Mean circulatory filling pressure

KW - Unstressed blood volume

KW - Vascular capacitance

UR - http://www.scopus.com/inward/record.url?scp=0034016121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034016121&partnerID=8YFLogxK

M3 - Article

C2 - 10666148

AN - SCOPUS:0034016121

VL - 278

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1857

IS - 2 47-2

ER -