Effects of epidermal growth factor, insulin and insulin-like growth factor I on rat pancreatic acinar cells cultured in serum-free medium

Patsy M. Brannon, Kendal Hirschi, Murray Korc

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The effects of epidermal growth factor (EGF), insulin, and insulinlike growth factor I (IGF-I) were examined alone and in combination on rat pancreatic acinar cells cultured 48 h in serum free medium. IGF-I at a concentration of 2.7 nM maintained viability of cultured acinar cells comparably to EGF. In contrast, insulin was less effective in maintaining acinar viability, even at high concentrations (170nM). There were no additive or interactive effects of these growth factors on acinar viability. EGF significantly increased [3H]-phenylalanine incorporation into acinar protein and the specific activity of phenylalanine-acylated transfer RNA (tRNAphe), but did not change the apparent rate of protein synthesis when compared with insulin or IGF-I. EGF with insulin, IGF-I, or both resulted in significantly lower specific activities of tRNAphewhen compared to EGF alone, but all had comparable rates of total phe-incorporation. Acinar cells readily degraded insulin, but not EGF or IGF-I. These results demonstrate some specificity in the acinar requirement for growth factors (EGF = IGF-I > insulin) in maintaining viability in culture.

Original languageEnglish (US)
Pages (from-to)41-48
Number of pages8
JournalPancreas
Volume3
Issue number1
DOIs
StatePublished - Feb 1988

Keywords

  • Acinar cells
  • Epidermal growth factor
  • Insulin
  • Insulin-like growth factor

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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