Effects of ethanol drinking on central nervous system functional activity of alcohol-preferring rats

Helen J K Sable, Zachary Rodd, Richard Bell, Jonathan A. Schultz, Larry Lumeng, William J. McBride

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The [14C]-2-deoxyglucose (2-DG) technique was used to assess the rates of local cerebral glucose utilization (LCGU) in key limbic, cerebral cortical, hippocampal, basal ganglionic, and subcortical regions of alcohol-preferring (P) rats following chronic 24-h free-choice ethanol drinking. Adult male P rats were submitted to (1) 8 continuous weeks of two-bottle access to 15% ethanol and water (E-C group); (2) 8 weeks of identical two-bottle access followed by 2 weeks of ethanol deprivation (E-D group); (3) cycles of 2 weeks of two-bottle ethanol access and 2 weeks of deprivation, repeated for four cycles (E-RD group); or (4) water only treatment [ethanol-naive group (E-N group)]. A single pulse of [14C]-2-DG (125 μCi/kg) was administered via a venous catheter, and timed arterial blood samples were collected over 45 min and later assayed for plasma glucose and [ 14C]-2-DG concentrations. Quantitative autoradiography was used to determine [14C] densities, and LCGU values were calculated. With the exception of a few small differences in the hippocampus, no significant differences were found in any of the central nervous system (CNS) regions examined among the four experimental groups of P rats. Animals in the E-D group had lower LCGU rates in the anterior hippocampal CA1 subregion than animals in the E-N, E-C, and E-RD groups. In the anterior hippocampal CA3 subregion and the anterior hippocampal dentate gyrus, the E-D group had significantly lower LCGU rates than the E-RD group. Overall, the results of this study indicate that 24-h ethanol-drinking experience has little effect on CNS functional neuronal activity in P rats.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalAlcohol
Volume35
Issue number2
DOIs
StatePublished - Feb 2005

Fingerprint

Neurology
Drinking
Rats
Ethanol
Central Nervous System
alcohol
Alcohols
Deoxyglucose
Bottles
Glucose
Group
utilization
Animals
deprivation
Parahippocampal Gyrus
Water
Water Purification
Catheters
Dentate Gyrus
animal

Keywords

  • [C]-2-deoxyglucose
  • Alcohol-deprivation effect
  • Alcohol-preferring (P) rats
  • Local cerebral glucose utilization
  • Quantitative autoradiography

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neuroscience(all)
  • Toxicology
  • Health(social science)

Cite this

Effects of ethanol drinking on central nervous system functional activity of alcohol-preferring rats. / Sable, Helen J K; Rodd, Zachary; Bell, Richard; Schultz, Jonathan A.; Lumeng, Larry; McBride, William J.

In: Alcohol, Vol. 35, No. 2, 02.2005, p. 129-135.

Research output: Contribution to journalArticle

Sable, Helen J K ; Rodd, Zachary ; Bell, Richard ; Schultz, Jonathan A. ; Lumeng, Larry ; McBride, William J. / Effects of ethanol drinking on central nervous system functional activity of alcohol-preferring rats. In: Alcohol. 2005 ; Vol. 35, No. 2. pp. 129-135.
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AB - The [14C]-2-deoxyglucose (2-DG) technique was used to assess the rates of local cerebral glucose utilization (LCGU) in key limbic, cerebral cortical, hippocampal, basal ganglionic, and subcortical regions of alcohol-preferring (P) rats following chronic 24-h free-choice ethanol drinking. Adult male P rats were submitted to (1) 8 continuous weeks of two-bottle access to 15% ethanol and water (E-C group); (2) 8 weeks of identical two-bottle access followed by 2 weeks of ethanol deprivation (E-D group); (3) cycles of 2 weeks of two-bottle ethanol access and 2 weeks of deprivation, repeated for four cycles (E-RD group); or (4) water only treatment [ethanol-naive group (E-N group)]. A single pulse of [14C]-2-DG (125 μCi/kg) was administered via a venous catheter, and timed arterial blood samples were collected over 45 min and later assayed for plasma glucose and [ 14C]-2-DG concentrations. Quantitative autoradiography was used to determine [14C] densities, and LCGU values were calculated. With the exception of a few small differences in the hippocampus, no significant differences were found in any of the central nervous system (CNS) regions examined among the four experimental groups of P rats. Animals in the E-D group had lower LCGU rates in the anterior hippocampal CA1 subregion than animals in the E-N, E-C, and E-RD groups. In the anterior hippocampal CA3 subregion and the anterior hippocampal dentate gyrus, the E-D group had significantly lower LCGU rates than the E-RD group. Overall, the results of this study indicate that 24-h ethanol-drinking experience has little effect on CNS functional neuronal activity in P rats.

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