Effects of ethanol exposure on subsequent acquisition and extinction of ethanol self-administration and expression of alcohol-seeking behavior in adult alcohol-preferring (P) rats: II. Adult exposure

Zachary Rodd, Richard Bell, Kelly A. Kuc, James M. Murphy, William J. McBride, Lawrence Lumeng, Ting Kai Li

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Background: In a preceding study, we reported that ethanol (EtOH) consumption during periadolescence in alcohol-preferring (P) rats produced significant effects on the acquisition, extinction, Pavlovian spontaneous recovery (PSR), and reacquisition of operant self-administration of EtOH. The objective of the present study was to determine if EtOH consumption during adulthood produced similar effects on subsequent operant behaviors. Methods: Adult female P rats (>135 days of age) were given 24 hr free-choice access to 15% EtOH for 30 days or were similarly housed and received water only. After a 15 day period of no EtOH access and without any prior training, adult alcohol drinking and adult alcohol-naïve rats were placed in standard two-lever (15% EtOH and water) chambers to examine acquisition of EtOH self-administration. After stable responding was established on a concurrent fixed ratio (FR) 5 FR1 schedule for EtOH versus water, the P rats underwent extinction training for nine sessions. After extinction and a 2 week home cage period (with no operant sessions or access to EtOH), rats were returned to the operant chambers in the absence of reward for seven consecutive sessions to test for PSR. After PSR testing, animals were maintained in their home cage for a week, before being reintroduced to the operant chambers and allowed to respond for EtOH and water. Results: Both the adult alcohol-drinking and adult alcohol-naïve groups rapidly acquired EtOH self-administration, expressed a pronounced PSR, which was augmented by EtOH priming and the presence of a discriminative stimulus (odor cue), and increased responding when EtOH was reinstated. Adult pre-exposure to EtOH did not alter any of the operant measures. Conclusions: The results of this study suggest that, unlike the results with EtOH pre-exposure during periadolescence, chronic alcohol drinking by P rats in adulthood did not produce sufficient long-lasting changes in neuronal function to alter subsequent operant acquisition of alcohol self-administration, alcohol relapse, or alcohol-seeking behavior.

Original languageEnglish
Pages (from-to)1642-1652
Number of pages11
JournalAlcoholism: Clinical and Experimental Research
Volume26
Issue number11
StatePublished - Nov 1 2002

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Self Administration
Rats
Ethanol
Alcohols
Alcohol Drinking
Water
Recovery
Reward
Cues
Psychological Extinction
Appointments and Schedules
Odors
Recurrence
Animals
Testing

Keywords

  • Alcohol Relapse
  • Alcohol-Preferring P Rats
  • Ethanol Acquisition
  • Extinction
  • Pavlovian Spontaneous Recovery

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Effects of ethanol exposure on subsequent acquisition and extinction of ethanol self-administration and expression of alcohol-seeking behavior in adult alcohol-preferring (P) rats : II. Adult exposure. / Rodd, Zachary; Bell, Richard; Kuc, Kelly A.; Murphy, James M.; McBride, William J.; Lumeng, Lawrence; Li, Ting Kai.

In: Alcoholism: Clinical and Experimental Research, Vol. 26, No. 11, 01.11.2002, p. 1642-1652.

Research output: Contribution to journalArticle

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abstract = "Background: In a preceding study, we reported that ethanol (EtOH) consumption during periadolescence in alcohol-preferring (P) rats produced significant effects on the acquisition, extinction, Pavlovian spontaneous recovery (PSR), and reacquisition of operant self-administration of EtOH. The objective of the present study was to determine if EtOH consumption during adulthood produced similar effects on subsequent operant behaviors. Methods: Adult female P rats (>135 days of age) were given 24 hr free-choice access to 15{\%} EtOH for 30 days or were similarly housed and received water only. After a 15 day period of no EtOH access and without any prior training, adult alcohol drinking and adult alcohol-na{\"i}ve rats were placed in standard two-lever (15{\%} EtOH and water) chambers to examine acquisition of EtOH self-administration. After stable responding was established on a concurrent fixed ratio (FR) 5 FR1 schedule for EtOH versus water, the P rats underwent extinction training for nine sessions. After extinction and a 2 week home cage period (with no operant sessions or access to EtOH), rats were returned to the operant chambers in the absence of reward for seven consecutive sessions to test for PSR. After PSR testing, animals were maintained in their home cage for a week, before being reintroduced to the operant chambers and allowed to respond for EtOH and water. Results: Both the adult alcohol-drinking and adult alcohol-na{\"i}ve groups rapidly acquired EtOH self-administration, expressed a pronounced PSR, which was augmented by EtOH priming and the presence of a discriminative stimulus (odor cue), and increased responding when EtOH was reinstated. Adult pre-exposure to EtOH did not alter any of the operant measures. Conclusions: The results of this study suggest that, unlike the results with EtOH pre-exposure during periadolescence, chronic alcohol drinking by P rats in adulthood did not produce sufficient long-lasting changes in neuronal function to alter subsequent operant acquisition of alcohol self-administration, alcohol relapse, or alcohol-seeking behavior.",
keywords = "Alcohol Relapse, Alcohol-Preferring P Rats, Ethanol Acquisition, Extinction, Pavlovian Spontaneous Recovery",
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T1 - Effects of ethanol exposure on subsequent acquisition and extinction of ethanol self-administration and expression of alcohol-seeking behavior in adult alcohol-preferring (P) rats

T2 - II. Adult exposure

AU - Rodd, Zachary

AU - Bell, Richard

AU - Kuc, Kelly A.

AU - Murphy, James M.

AU - McBride, William J.

AU - Lumeng, Lawrence

AU - Li, Ting Kai

PY - 2002/11/1

Y1 - 2002/11/1

N2 - Background: In a preceding study, we reported that ethanol (EtOH) consumption during periadolescence in alcohol-preferring (P) rats produced significant effects on the acquisition, extinction, Pavlovian spontaneous recovery (PSR), and reacquisition of operant self-administration of EtOH. The objective of the present study was to determine if EtOH consumption during adulthood produced similar effects on subsequent operant behaviors. Methods: Adult female P rats (>135 days of age) were given 24 hr free-choice access to 15% EtOH for 30 days or were similarly housed and received water only. After a 15 day period of no EtOH access and without any prior training, adult alcohol drinking and adult alcohol-naïve rats were placed in standard two-lever (15% EtOH and water) chambers to examine acquisition of EtOH self-administration. After stable responding was established on a concurrent fixed ratio (FR) 5 FR1 schedule for EtOH versus water, the P rats underwent extinction training for nine sessions. After extinction and a 2 week home cage period (with no operant sessions or access to EtOH), rats were returned to the operant chambers in the absence of reward for seven consecutive sessions to test for PSR. After PSR testing, animals were maintained in their home cage for a week, before being reintroduced to the operant chambers and allowed to respond for EtOH and water. Results: Both the adult alcohol-drinking and adult alcohol-naïve groups rapidly acquired EtOH self-administration, expressed a pronounced PSR, which was augmented by EtOH priming and the presence of a discriminative stimulus (odor cue), and increased responding when EtOH was reinstated. Adult pre-exposure to EtOH did not alter any of the operant measures. Conclusions: The results of this study suggest that, unlike the results with EtOH pre-exposure during periadolescence, chronic alcohol drinking by P rats in adulthood did not produce sufficient long-lasting changes in neuronal function to alter subsequent operant acquisition of alcohol self-administration, alcohol relapse, or alcohol-seeking behavior.

AB - Background: In a preceding study, we reported that ethanol (EtOH) consumption during periadolescence in alcohol-preferring (P) rats produced significant effects on the acquisition, extinction, Pavlovian spontaneous recovery (PSR), and reacquisition of operant self-administration of EtOH. The objective of the present study was to determine if EtOH consumption during adulthood produced similar effects on subsequent operant behaviors. Methods: Adult female P rats (>135 days of age) were given 24 hr free-choice access to 15% EtOH for 30 days or were similarly housed and received water only. After a 15 day period of no EtOH access and without any prior training, adult alcohol drinking and adult alcohol-naïve rats were placed in standard two-lever (15% EtOH and water) chambers to examine acquisition of EtOH self-administration. After stable responding was established on a concurrent fixed ratio (FR) 5 FR1 schedule for EtOH versus water, the P rats underwent extinction training for nine sessions. After extinction and a 2 week home cage period (with no operant sessions or access to EtOH), rats were returned to the operant chambers in the absence of reward for seven consecutive sessions to test for PSR. After PSR testing, animals were maintained in their home cage for a week, before being reintroduced to the operant chambers and allowed to respond for EtOH and water. Results: Both the adult alcohol-drinking and adult alcohol-naïve groups rapidly acquired EtOH self-administration, expressed a pronounced PSR, which was augmented by EtOH priming and the presence of a discriminative stimulus (odor cue), and increased responding when EtOH was reinstated. Adult pre-exposure to EtOH did not alter any of the operant measures. Conclusions: The results of this study suggest that, unlike the results with EtOH pre-exposure during periadolescence, chronic alcohol drinking by P rats in adulthood did not produce sufficient long-lasting changes in neuronal function to alter subsequent operant acquisition of alcohol self-administration, alcohol relapse, or alcohol-seeking behavior.

KW - Alcohol Relapse

KW - Alcohol-Preferring P Rats

KW - Ethanol Acquisition

KW - Extinction

KW - Pavlovian Spontaneous Recovery

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