Effects of ethanol on monoamine and amino acid release from cerebral cortical slices of the alcohol-preferring P line of rats

W. J. McBride, J. M. Murphy, L. Lumeng, T. K. Li

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Abstract

The effects of 250 mg/100 ml ethanol on the efflux of 3,4-dihydroxyphenylacetic acid (DOPAC) and the 35 mM K+-stimulated, Ca2+-dependent release of norepinephrine (NE), dopamine (DA), serotonin (5-HT), γ-aminobutyric acid (GABA), glutamate, and aspartate from cerebral cortical slices of the alcohol-preferring P line of rats and stock Wistar rats were studied. The K+-stimulated, Ca2+-dependent release of GABA for the P rats was 35% lower, while the release of glutamate was almost twice that of the stock animals. The release of the other compounds was similar for the two groups. Addition of 250 mg/100 ml ethanol to the superfusion media did not alter the release of NE, DA, and 5-HT nor the efflux of DOPAC from cortical slices of either group of rats. However, the K+-stimulated, Ca2+-dependent release of GABA, glutamate, and aspartate was significantly enhanced by ethanol for both the P and stock group of rats. These in vitro data do not support a direct action of ethanol on DA, NE, and 5-HT release or on DOPAC efflux, but suggest a direct action on the transmitter release process for GABA, glutamate, and aspartate in the cerebral cortex.

Original languageEnglish
Pages (from-to)205-208
Number of pages4
JournalAlcoholism: Clinical and Experimental Research
Volume10
Issue number2
StatePublished - 1986

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3,4-Dihydroxyphenylacetic Acid
Rats
Glutamic Acid
Serotonin
Ethanol
Alcohols
Aspartic Acid
gamma-Aminobutyric Acid
Amino Acids
Dopamine
Norepinephrine
Aminobutyrates
Cerebral Cortex
Wistar Rats
Transmitters
Animals

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

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Effects of ethanol on monoamine and amino acid release from cerebral cortical slices of the alcohol-preferring P line of rats. / McBride, W. J.; Murphy, J. M.; Lumeng, L.; Li, T. K.

In: Alcoholism: Clinical and Experimental Research, Vol. 10, No. 2, 1986, p. 205-208.

Research output: Contribution to journalArticle

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