Effects of ethanol on rat somatosensory cortical neurons

Francis M. Sessler, Fu Chun Hsu, Takiyah N. Felder, Jin Zhai, Rick C S Lin, Steven J. Wieland, Ann Kosobud

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study, we characterized the local effects of ethanol (EtOH) on postsynaptic potentials (PSPs) and membrane properties of layer II-III (L2- 3) and layer V (L5) somatosensory cortical neurons. Intracellular recordings were done using the in vitro slice preparation of rat somatosensory cortex. Our results show that EtOH exerts local effects on cortical cell membrane at physiologically relevant concentrations. A predominant effect of EtOH was to reduce excitability of L2-3 and L5 neurons by increasing the rheobase, decreasing input resistance and repetitive firing, reducing PSPs amplitude and the probability of evoking action potentials. Early (6 ms) and late (18 ms) PSP components were affected differentially by EtOH, the late components being more suppressed. Overall, EtOH-mediated suppression of PSPs was stronger in L5 neurons. Cortical neurons were divided into three subtypes: regular spiking adapting (RS-A), regular spiking non-adapting (RS-NA) and bursting (D-IB) neurons. PSPs evoked in RS-A neurons were more sensitive to EtOH suppressant effects. EtOH effects on input resistance were distributed differentially among the three groups of neurons. These results support the notion that EtOH disrupts higher processing of somatosensory information via a differential alteration of cortical neuron's membrane properties and synaptic transmission.

Original languageEnglish
Pages (from-to)266-274
Number of pages9
JournalBrain Research
Volume804
Issue number2
DOIs
StatePublished - Sep 7 1998

Fingerprint

Ethanol
Synaptic Potentials
Neurons
Somatosensory Cortex
Membranes
Automatic Data Processing
Synaptic Transmission
Action Potentials
Cell Membrane

Keywords

  • Ethanol
  • Intracellular recording
  • Membrane property
  • Pyramidal neuron
  • Sensory processing
  • Somatosensory cortex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sessler, F. M., Hsu, F. C., Felder, T. N., Zhai, J., Lin, R. C. S., Wieland, S. J., & Kosobud, A. (1998). Effects of ethanol on rat somatosensory cortical neurons. Brain Research, 804(2), 266-274. https://doi.org/10.1016/S0006-8993(98)00680-5

Effects of ethanol on rat somatosensory cortical neurons. / Sessler, Francis M.; Hsu, Fu Chun; Felder, Takiyah N.; Zhai, Jin; Lin, Rick C S; Wieland, Steven J.; Kosobud, Ann.

In: Brain Research, Vol. 804, No. 2, 07.09.1998, p. 266-274.

Research output: Contribution to journalArticle

Sessler, FM, Hsu, FC, Felder, TN, Zhai, J, Lin, RCS, Wieland, SJ & Kosobud, A 1998, 'Effects of ethanol on rat somatosensory cortical neurons', Brain Research, vol. 804, no. 2, pp. 266-274. https://doi.org/10.1016/S0006-8993(98)00680-5
Sessler FM, Hsu FC, Felder TN, Zhai J, Lin RCS, Wieland SJ et al. Effects of ethanol on rat somatosensory cortical neurons. Brain Research. 1998 Sep 7;804(2):266-274. https://doi.org/10.1016/S0006-8993(98)00680-5
Sessler, Francis M. ; Hsu, Fu Chun ; Felder, Takiyah N. ; Zhai, Jin ; Lin, Rick C S ; Wieland, Steven J. ; Kosobud, Ann. / Effects of ethanol on rat somatosensory cortical neurons. In: Brain Research. 1998 ; Vol. 804, No. 2. pp. 266-274.
@article{8279f0d9a3e34293bf3f217a356864bc,
title = "Effects of ethanol on rat somatosensory cortical neurons",
abstract = "In this study, we characterized the local effects of ethanol (EtOH) on postsynaptic potentials (PSPs) and membrane properties of layer II-III (L2- 3) and layer V (L5) somatosensory cortical neurons. Intracellular recordings were done using the in vitro slice preparation of rat somatosensory cortex. Our results show that EtOH exerts local effects on cortical cell membrane at physiologically relevant concentrations. A predominant effect of EtOH was to reduce excitability of L2-3 and L5 neurons by increasing the rheobase, decreasing input resistance and repetitive firing, reducing PSPs amplitude and the probability of evoking action potentials. Early (6 ms) and late (18 ms) PSP components were affected differentially by EtOH, the late components being more suppressed. Overall, EtOH-mediated suppression of PSPs was stronger in L5 neurons. Cortical neurons were divided into three subtypes: regular spiking adapting (RS-A), regular spiking non-adapting (RS-NA) and bursting (D-IB) neurons. PSPs evoked in RS-A neurons were more sensitive to EtOH suppressant effects. EtOH effects on input resistance were distributed differentially among the three groups of neurons. These results support the notion that EtOH disrupts higher processing of somatosensory information via a differential alteration of cortical neuron's membrane properties and synaptic transmission.",
keywords = "Ethanol, Intracellular recording, Membrane property, Pyramidal neuron, Sensory processing, Somatosensory cortex",
author = "Sessler, {Francis M.} and Hsu, {Fu Chun} and Felder, {Takiyah N.} and Jin Zhai and Lin, {Rick C S} and Wieland, {Steven J.} and Ann Kosobud",
year = "1998",
month = "9",
day = "7",
doi = "10.1016/S0006-8993(98)00680-5",
language = "English",
volume = "804",
pages = "266--274",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Effects of ethanol on rat somatosensory cortical neurons

AU - Sessler, Francis M.

AU - Hsu, Fu Chun

AU - Felder, Takiyah N.

AU - Zhai, Jin

AU - Lin, Rick C S

AU - Wieland, Steven J.

AU - Kosobud, Ann

PY - 1998/9/7

Y1 - 1998/9/7

N2 - In this study, we characterized the local effects of ethanol (EtOH) on postsynaptic potentials (PSPs) and membrane properties of layer II-III (L2- 3) and layer V (L5) somatosensory cortical neurons. Intracellular recordings were done using the in vitro slice preparation of rat somatosensory cortex. Our results show that EtOH exerts local effects on cortical cell membrane at physiologically relevant concentrations. A predominant effect of EtOH was to reduce excitability of L2-3 and L5 neurons by increasing the rheobase, decreasing input resistance and repetitive firing, reducing PSPs amplitude and the probability of evoking action potentials. Early (6 ms) and late (18 ms) PSP components were affected differentially by EtOH, the late components being more suppressed. Overall, EtOH-mediated suppression of PSPs was stronger in L5 neurons. Cortical neurons were divided into three subtypes: regular spiking adapting (RS-A), regular spiking non-adapting (RS-NA) and bursting (D-IB) neurons. PSPs evoked in RS-A neurons were more sensitive to EtOH suppressant effects. EtOH effects on input resistance were distributed differentially among the three groups of neurons. These results support the notion that EtOH disrupts higher processing of somatosensory information via a differential alteration of cortical neuron's membrane properties and synaptic transmission.

AB - In this study, we characterized the local effects of ethanol (EtOH) on postsynaptic potentials (PSPs) and membrane properties of layer II-III (L2- 3) and layer V (L5) somatosensory cortical neurons. Intracellular recordings were done using the in vitro slice preparation of rat somatosensory cortex. Our results show that EtOH exerts local effects on cortical cell membrane at physiologically relevant concentrations. A predominant effect of EtOH was to reduce excitability of L2-3 and L5 neurons by increasing the rheobase, decreasing input resistance and repetitive firing, reducing PSPs amplitude and the probability of evoking action potentials. Early (6 ms) and late (18 ms) PSP components were affected differentially by EtOH, the late components being more suppressed. Overall, EtOH-mediated suppression of PSPs was stronger in L5 neurons. Cortical neurons were divided into three subtypes: regular spiking adapting (RS-A), regular spiking non-adapting (RS-NA) and bursting (D-IB) neurons. PSPs evoked in RS-A neurons were more sensitive to EtOH suppressant effects. EtOH effects on input resistance were distributed differentially among the three groups of neurons. These results support the notion that EtOH disrupts higher processing of somatosensory information via a differential alteration of cortical neuron's membrane properties and synaptic transmission.

KW - Ethanol

KW - Intracellular recording

KW - Membrane property

KW - Pyramidal neuron

KW - Sensory processing

KW - Somatosensory cortex

UR - http://www.scopus.com/inward/record.url?scp=0032494266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032494266&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(98)00680-5

DO - 10.1016/S0006-8993(98)00680-5

M3 - Article

C2 - 9757061

AN - SCOPUS:0032494266

VL - 804

SP - 266

EP - 274

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -