Effects of ethanol on startle responding in alcohol-preferring and -non-preferring rats

A. E. Jones, W. J. McBride, J. M. Murphy, L. Lumeng, T. K. Li, Anantha Shekhar, D. L. McKinzie

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The objectives of the present study were to determine (a) if differences exist between the selectively bred alcohol-preferring (P) and -non-preferring (NP) lines of rats in the acoustic startle response (ASR) and prepulse inhibition (PPI), and (b) the effects of ethanol on these measures. Alcohol-naive adult female P and NP rats received a single i.p. injection of saline or ethanol (0.25, 0.5, 1.0, or 1.5 g/kg) and were placed in the startle apparatus 10 min later. After a 5-min acclimation period, rats received five alternating trials of a startle stimulus alone (SSA) (115-dB white noise) or a PPI trial (90-dB white noise preceding a 115-dB white noise). Analysis of the ASR revealed that P rats exhibited higher startle amplitudes than did NP rats with saline injections. The 0.5-g/kg ethanol dose reduced the startle amplitude in P, but not NP, rats. The 1.0- and 1.5-g/kg ethanol doses nearly abolished the ASR in the NP line, whereas only the highest ethanol dose had this effect in the P line. Vehicle-treated P and NP rats exhibited comparable PPI levels, but only P rats showed a significant disruption (30%) at the 0.50-g/kg ethanol dose. Neither P nor NP rats were affected by ethanol treatment at the 0.25-g/kg dose. Overall, the results suggest that: (a) the difference in baseline ASR may indicate line differences in the neurocircuitry mediating this response, possibly reflecting higher innate levels of emotional reactivity in the P line; (b) the P line may be more sensitive than the NP line to the effects of ethanol in reducing emotional reactivity; and (c) low-dose ethanol may have a greater disruptive effect on sensorimotor gating mechanisms in the P than NP rat. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalPharmacology Biochemistry and Behavior
Volume67
Issue number2
DOIs
StatePublished - 2000

Fingerprint

Rats
Ethanol
Alcohols
Startle Reflex
Acoustics
Sensory Gating
Injections
Acclimatization
Prepulse Inhibition

Keywords

  • Acoustic startle response
  • Alcohol-non-preferring NP rats
  • Alcohol-preferring P rats
  • Anxiety
  • Prepulse inhibition
  • Sensorimotor gating

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this

Effects of ethanol on startle responding in alcohol-preferring and -non-preferring rats. / Jones, A. E.; McBride, W. J.; Murphy, J. M.; Lumeng, L.; Li, T. K.; Shekhar, Anantha; McKinzie, D. L.

In: Pharmacology Biochemistry and Behavior, Vol. 67, No. 2, 2000, p. 313-318.

Research output: Contribution to journalArticle

Jones, A. E. ; McBride, W. J. ; Murphy, J. M. ; Lumeng, L. ; Li, T. K. ; Shekhar, Anantha ; McKinzie, D. L. / Effects of ethanol on startle responding in alcohol-preferring and -non-preferring rats. In: Pharmacology Biochemistry and Behavior. 2000 ; Vol. 67, No. 2. pp. 313-318.
@article{a010b0f5927747da9e43a3a58133b7a9,
title = "Effects of ethanol on startle responding in alcohol-preferring and -non-preferring rats",
abstract = "The objectives of the present study were to determine (a) if differences exist between the selectively bred alcohol-preferring (P) and -non-preferring (NP) lines of rats in the acoustic startle response (ASR) and prepulse inhibition (PPI), and (b) the effects of ethanol on these measures. Alcohol-naive adult female P and NP rats received a single i.p. injection of saline or ethanol (0.25, 0.5, 1.0, or 1.5 g/kg) and were placed in the startle apparatus 10 min later. After a 5-min acclimation period, rats received five alternating trials of a startle stimulus alone (SSA) (115-dB white noise) or a PPI trial (90-dB white noise preceding a 115-dB white noise). Analysis of the ASR revealed that P rats exhibited higher startle amplitudes than did NP rats with saline injections. The 0.5-g/kg ethanol dose reduced the startle amplitude in P, but not NP, rats. The 1.0- and 1.5-g/kg ethanol doses nearly abolished the ASR in the NP line, whereas only the highest ethanol dose had this effect in the P line. Vehicle-treated P and NP rats exhibited comparable PPI levels, but only P rats showed a significant disruption (30{\%}) at the 0.50-g/kg ethanol dose. Neither P nor NP rats were affected by ethanol treatment at the 0.25-g/kg dose. Overall, the results suggest that: (a) the difference in baseline ASR may indicate line differences in the neurocircuitry mediating this response, possibly reflecting higher innate levels of emotional reactivity in the P line; (b) the P line may be more sensitive than the NP line to the effects of ethanol in reducing emotional reactivity; and (c) low-dose ethanol may have a greater disruptive effect on sensorimotor gating mechanisms in the P than NP rat. Copyright (C) 2000 Elsevier Science Inc.",
keywords = "Acoustic startle response, Alcohol-non-preferring NP rats, Alcohol-preferring P rats, Anxiety, Prepulse inhibition, Sensorimotor gating",
author = "Jones, {A. E.} and McBride, {W. J.} and Murphy, {J. M.} and L. Lumeng and Li, {T. K.} and Anantha Shekhar and McKinzie, {D. L.}",
year = "2000",
doi = "10.1016/S0091-3057(00)00363-4",
language = "English",
volume = "67",
pages = "313--318",
journal = "Pharmacology, Biochemistry and Behavior",
issn = "0091-3057",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Effects of ethanol on startle responding in alcohol-preferring and -non-preferring rats

AU - Jones, A. E.

AU - McBride, W. J.

AU - Murphy, J. M.

AU - Lumeng, L.

AU - Li, T. K.

AU - Shekhar, Anantha

AU - McKinzie, D. L.

PY - 2000

Y1 - 2000

N2 - The objectives of the present study were to determine (a) if differences exist between the selectively bred alcohol-preferring (P) and -non-preferring (NP) lines of rats in the acoustic startle response (ASR) and prepulse inhibition (PPI), and (b) the effects of ethanol on these measures. Alcohol-naive adult female P and NP rats received a single i.p. injection of saline or ethanol (0.25, 0.5, 1.0, or 1.5 g/kg) and were placed in the startle apparatus 10 min later. After a 5-min acclimation period, rats received five alternating trials of a startle stimulus alone (SSA) (115-dB white noise) or a PPI trial (90-dB white noise preceding a 115-dB white noise). Analysis of the ASR revealed that P rats exhibited higher startle amplitudes than did NP rats with saline injections. The 0.5-g/kg ethanol dose reduced the startle amplitude in P, but not NP, rats. The 1.0- and 1.5-g/kg ethanol doses nearly abolished the ASR in the NP line, whereas only the highest ethanol dose had this effect in the P line. Vehicle-treated P and NP rats exhibited comparable PPI levels, but only P rats showed a significant disruption (30%) at the 0.50-g/kg ethanol dose. Neither P nor NP rats were affected by ethanol treatment at the 0.25-g/kg dose. Overall, the results suggest that: (a) the difference in baseline ASR may indicate line differences in the neurocircuitry mediating this response, possibly reflecting higher innate levels of emotional reactivity in the P line; (b) the P line may be more sensitive than the NP line to the effects of ethanol in reducing emotional reactivity; and (c) low-dose ethanol may have a greater disruptive effect on sensorimotor gating mechanisms in the P than NP rat. Copyright (C) 2000 Elsevier Science Inc.

AB - The objectives of the present study were to determine (a) if differences exist between the selectively bred alcohol-preferring (P) and -non-preferring (NP) lines of rats in the acoustic startle response (ASR) and prepulse inhibition (PPI), and (b) the effects of ethanol on these measures. Alcohol-naive adult female P and NP rats received a single i.p. injection of saline or ethanol (0.25, 0.5, 1.0, or 1.5 g/kg) and were placed in the startle apparatus 10 min later. After a 5-min acclimation period, rats received five alternating trials of a startle stimulus alone (SSA) (115-dB white noise) or a PPI trial (90-dB white noise preceding a 115-dB white noise). Analysis of the ASR revealed that P rats exhibited higher startle amplitudes than did NP rats with saline injections. The 0.5-g/kg ethanol dose reduced the startle amplitude in P, but not NP, rats. The 1.0- and 1.5-g/kg ethanol doses nearly abolished the ASR in the NP line, whereas only the highest ethanol dose had this effect in the P line. Vehicle-treated P and NP rats exhibited comparable PPI levels, but only P rats showed a significant disruption (30%) at the 0.50-g/kg ethanol dose. Neither P nor NP rats were affected by ethanol treatment at the 0.25-g/kg dose. Overall, the results suggest that: (a) the difference in baseline ASR may indicate line differences in the neurocircuitry mediating this response, possibly reflecting higher innate levels of emotional reactivity in the P line; (b) the P line may be more sensitive than the NP line to the effects of ethanol in reducing emotional reactivity; and (c) low-dose ethanol may have a greater disruptive effect on sensorimotor gating mechanisms in the P than NP rat. Copyright (C) 2000 Elsevier Science Inc.

KW - Acoustic startle response

KW - Alcohol-non-preferring NP rats

KW - Alcohol-preferring P rats

KW - Anxiety

KW - Prepulse inhibition

KW - Sensorimotor gating

UR - http://www.scopus.com/inward/record.url?scp=0034535961&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034535961&partnerID=8YFLogxK

U2 - 10.1016/S0091-3057(00)00363-4

DO - 10.1016/S0091-3057(00)00363-4

M3 - Article

VL - 67

SP - 313

EP - 318

JO - Pharmacology, Biochemistry and Behavior

JF - Pharmacology, Biochemistry and Behavior

SN - 0091-3057

IS - 2

ER -