Effects of extracellular matrix molecules on the growth properties of oligodendrocyte progenitor cells in vitro

Jianguo Hu, Lingxiao Deng, Xiaofei Wang, Xiao-Ming Xu

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The extracellular matrix (ECM) is a component of neural cell niches and regulates multiple functions of diverse cell types. To date, limited information is available concerning its biological effects on the growth properties of oligodendrocyte progenitor cells (OPCs). In the present study, we examined effects of several ECM components, i.e., fibronectin, laminin, and Matrigel, on the survival, proliferation, migration, process extension, and purity of OPCs isolated from embryonic day 15 rat spinal cords. All three ECM components enhanced these biological properties of the OPCs compared with a non-ECM substrate, poly-D-lysine. However, the extents of their effects were somewhat different. Among these ECMs, fibronectin showed the strongest effect on almost all aspects of the growth properties of OPCs, implying that this molecule is a better substrate for the growth of OPCs in vitro. Because of its survivaland growth-promoting effects on OPCs, fibronectin may be considered as a candidate substrate for enhancing OPC-mediated repair under conditions when exogenous delivery or endogenous stimulation of OPCs is applied.

Original languageEnglish
Pages (from-to)2854-2862
Number of pages9
JournalJournal of Neuroscience Research
Volume87
Issue number13
DOIs
StatePublished - Oct 2009

Fingerprint

Oligodendroglia
Extracellular Matrix
Stem Cells
Growth
Fibronectins
In Vitro Techniques
Laminin
Cellular Structures
Lysine
Spinal Cord

Keywords

  • Differentiation
  • Extracellular matrix
  • Migration
  • Oligodendrocyte progenitor cells
  • Process length
  • Proliferation
  • Survival

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Effects of extracellular matrix molecules on the growth properties of oligodendrocyte progenitor cells in vitro. / Hu, Jianguo; Deng, Lingxiao; Wang, Xiaofei; Xu, Xiao-Ming.

In: Journal of Neuroscience Research, Vol. 87, No. 13, 10.2009, p. 2854-2862.

Research output: Contribution to journalArticle

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