Effects of extracellular matrix molecules on the growth properties of oligodendrocyte progenitor cells in vitro

Jianguo Hu, Lingxiao Deng, Xiaofei Wang, Xiao Ming Xu

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The extracellular matrix (ECM) is a component of neural cell niches and regulates multiple functions of diverse cell types. To date, limited information is available concerning its biological effects on the growth properties of oligodendrocyte progenitor cells (OPCs). In the present study, we examined effects of several ECM components, i.e., fibronectin, laminin, and Matrigel, on the survival, proliferation, migration, process extension, and purity of OPCs isolated from embryonic day 15 rat spinal cords. All three ECM components enhanced these biological properties of the OPCs compared with a non-ECM substrate, poly-D-lysine. However, the extents of their effects were somewhat different. Among these ECMs, fibronectin showed the strongest effect on almost all aspects of the growth properties of OPCs, implying that this molecule is a better substrate for the growth of OPCs in vitro. Because of its survivaland growth-promoting effects on OPCs, fibronectin may be considered as a candidate substrate for enhancing OPC-mediated repair under conditions when exogenous delivery or endogenous stimulation of OPCs is applied.

Original languageEnglish (US)
Pages (from-to)2854-2862
Number of pages9
JournalJournal of Neuroscience Research
Volume87
Issue number13
DOIs
StatePublished - Oct 1 2009

Keywords

  • Differentiation
  • Extracellular matrix
  • Migration
  • Oligodendrocyte progenitor cells
  • Process length
  • Proliferation
  • Survival

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Effects of extracellular matrix molecules on the growth properties of oligodendrocyte progenitor cells in vitro'. Together they form a unique fingerprint.

  • Cite this