Effects of heat shock on the Mre11/Rad50/Nbs1 complex in irradiated or unirradiated cells

Joseph Dynlacht, M. Xu, R. K. Pandita, E. A. Wetzel, J. L. Roti Roti

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The mechanism by which hyperthermia sensitizes mammalian cells to ionizing radiation remains to be elucidated, but an overwhelming amount of circumstantial evidence suggests that heat radiosensitization might be mediated by inhibition of double-strand break repair, particularly after exposure of irradiated cells to heat treatments in excess of about 43°C. In mammalian cells, double-strand break repair usually occurs via two pathways, non-homologous end-joining and homologous recombination. Several reports suggest a role for non-homologous end-joining in heat radiosensitization, while others implicate homologous recombination as a target. However, cell lines that are compromised in either the non-homologous end-joining or homologous recombination pathway are still capable of being radiosensitized, suggesting that heat affects both pathways. Indeed, several of the proteins involved in one or both of these pathways have been observed to undergo alterations or translocation after unirradiated or irradiated cells are exposed to heat shock. The work summarized in this review implicates proteins of the Mre11/Rad50/Nbs1 complex as targets for heat radiosensitization.

Original languageEnglish
Pages (from-to)144-156
Number of pages13
JournalInternational Journal of Hyperthermia
Volume20
Issue number2
DOIs
StatePublished - Mar 2004

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Shock
Hot Temperature
Homologous Recombination
Ionizing Radiation
Proteins
Fever
Cell Line

Keywords

  • Double-strand breaks
  • Homologous recombination
  • Hyperthermia
  • Radiation

ASJC Scopus subject areas

  • Cancer Research
  • Radiological and Ultrasound Technology

Cite this

Effects of heat shock on the Mre11/Rad50/Nbs1 complex in irradiated or unirradiated cells. / Dynlacht, Joseph; Xu, M.; Pandita, R. K.; Wetzel, E. A.; Roti Roti, J. L.

In: International Journal of Hyperthermia, Vol. 20, No. 2, 03.2004, p. 144-156.

Research output: Contribution to journalArticle

Dynlacht, Joseph ; Xu, M. ; Pandita, R. K. ; Wetzel, E. A. ; Roti Roti, J. L. / Effects of heat shock on the Mre11/Rad50/Nbs1 complex in irradiated or unirradiated cells. In: International Journal of Hyperthermia. 2004 ; Vol. 20, No. 2. pp. 144-156.
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