Effects of high-intensity focused ultrasound in the treatment of experimental neuroblastoma

Rong Yang, Clarence R. Reilly, Frederick Rescorla, Narendra T. Sanghvi, Francis J. Fry, Thomas D. Franklin, Jay L. Grosfeld

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Abstract

This report evaluates the effect of high-intensity focused ultrasound (HIFU) on subcutaneous murine neuroblastoma C1300. HIFU treatment was administered with a focused 4-MHz quartz transducer with a peak intensity of 550 W/cm2. In experiment 1, 60 animals with tumor were divided into four groups. Group I (n = 15) were controls; group II (n = 15) received adriamycin, 5 mg/kg intraperitoneally; group III (n = 15) received HIFU; and group IV (n = 15) received both adriamycin and HIFU. All the animals in groups I and II died of tumor by 35 days. Fifty-three percent ( 8 15) of mice in group III and 80% ( 12 15) in group IV were cured with no evidence of tumor (NET) at 200 days. Log-rank statistics showed significant prolongation of survival in the groups III and IV as compared with groups I or II (P < .05). In experiment 2, 45 animals with tumor were divided into three groups. Group I (n = 15) were controls; group II (n = 15) received HIFU; and group III (n = 15) received repeated HIFU. The results showed 47% ( 7 15) of mice in group II and 67% ( 10 15) in group III were NET at 200 days. Significant survival prolongation was achieved in groups II and III in comparison with group I (P < .05). In experiment 3, 90 mice received either tumor (n = 60) or saline (n = 30) inoculation in the left flank. On day 5, 45 mice with tumor were treated with HIFU (group I), while the other 15 mice with tumor (group II) had a sham procedure. Nineteen mice in group I were cured with NET (group IA) and 26 had persistent tumor (group IB). The 30 mice receiving saline (without tumor) were treated with either HIFU (group III, n = 15) or a sham procedure (group IV, n = 15). On day 26, all the animals received a second tumor challenge in the right flank. Reduced tumor growth following a second tumor challenge was demonstrated in group IA as compared with other groups (P < .001), implying a stimulation of host tumor immunity following curative HIFU treatment. The data suggest that HIFU may be an alternative modality for the treatment of unresectable neuroblastoma.

Original languageEnglish
Pages (from-to)246-251
Number of pages6
JournalJournal of Pediatric Surgery
Volume27
Issue number2
DOIs
StatePublished - 1992

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Neuroblastoma
Neoplasms
Doxorubicin
Quartz
Transducers
Immunity

Keywords

  • high-intensity ultrasound
  • Neuroblastoma

ASJC Scopus subject areas

  • Surgery

Cite this

Effects of high-intensity focused ultrasound in the treatment of experimental neuroblastoma. / Yang, Rong; Reilly, Clarence R.; Rescorla, Frederick; Sanghvi, Narendra T.; Fry, Francis J.; Franklin, Thomas D.; Grosfeld, Jay L.

In: Journal of Pediatric Surgery, Vol. 27, No. 2, 1992, p. 246-251.

Research output: Contribution to journalArticle

Yang, Rong ; Reilly, Clarence R. ; Rescorla, Frederick ; Sanghvi, Narendra T. ; Fry, Francis J. ; Franklin, Thomas D. ; Grosfeld, Jay L. / Effects of high-intensity focused ultrasound in the treatment of experimental neuroblastoma. In: Journal of Pediatric Surgery. 1992 ; Vol. 27, No. 2. pp. 246-251.
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abstract = "This report evaluates the effect of high-intensity focused ultrasound (HIFU) on subcutaneous murine neuroblastoma C1300. HIFU treatment was administered with a focused 4-MHz quartz transducer with a peak intensity of 550 W/cm2. In experiment 1, 60 animals with tumor were divided into four groups. Group I (n = 15) were controls; group II (n = 15) received adriamycin, 5 mg/kg intraperitoneally; group III (n = 15) received HIFU; and group IV (n = 15) received both adriamycin and HIFU. All the animals in groups I and II died of tumor by 35 days. Fifty-three percent ( 8 15) of mice in group III and 80{\%} ( 12 15) in group IV were cured with no evidence of tumor (NET) at 200 days. Log-rank statistics showed significant prolongation of survival in the groups III and IV as compared with groups I or II (P < .05). In experiment 2, 45 animals with tumor were divided into three groups. Group I (n = 15) were controls; group II (n = 15) received HIFU; and group III (n = 15) received repeated HIFU. The results showed 47{\%} ( 7 15) of mice in group II and 67{\%} ( 10 15) in group III were NET at 200 days. Significant survival prolongation was achieved in groups II and III in comparison with group I (P < .05). In experiment 3, 90 mice received either tumor (n = 60) or saline (n = 30) inoculation in the left flank. On day 5, 45 mice with tumor were treated with HIFU (group I), while the other 15 mice with tumor (group II) had a sham procedure. Nineteen mice in group I were cured with NET (group IA) and 26 had persistent tumor (group IB). The 30 mice receiving saline (without tumor) were treated with either HIFU (group III, n = 15) or a sham procedure (group IV, n = 15). On day 26, all the animals received a second tumor challenge in the right flank. Reduced tumor growth following a second tumor challenge was demonstrated in group IA as compared with other groups (P < .001), implying a stimulation of host tumor immunity following curative HIFU treatment. The data suggest that HIFU may be an alternative modality for the treatment of unresectable neuroblastoma.",
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AU - Sanghvi, Narendra T.

AU - Fry, Francis J.

AU - Franklin, Thomas D.

AU - Grosfeld, Jay L.

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N2 - This report evaluates the effect of high-intensity focused ultrasound (HIFU) on subcutaneous murine neuroblastoma C1300. HIFU treatment was administered with a focused 4-MHz quartz transducer with a peak intensity of 550 W/cm2. In experiment 1, 60 animals with tumor were divided into four groups. Group I (n = 15) were controls; group II (n = 15) received adriamycin, 5 mg/kg intraperitoneally; group III (n = 15) received HIFU; and group IV (n = 15) received both adriamycin and HIFU. All the animals in groups I and II died of tumor by 35 days. Fifty-three percent ( 8 15) of mice in group III and 80% ( 12 15) in group IV were cured with no evidence of tumor (NET) at 200 days. Log-rank statistics showed significant prolongation of survival in the groups III and IV as compared with groups I or II (P < .05). In experiment 2, 45 animals with tumor were divided into three groups. Group I (n = 15) were controls; group II (n = 15) received HIFU; and group III (n = 15) received repeated HIFU. The results showed 47% ( 7 15) of mice in group II and 67% ( 10 15) in group III were NET at 200 days. Significant survival prolongation was achieved in groups II and III in comparison with group I (P < .05). In experiment 3, 90 mice received either tumor (n = 60) or saline (n = 30) inoculation in the left flank. On day 5, 45 mice with tumor were treated with HIFU (group I), while the other 15 mice with tumor (group II) had a sham procedure. Nineteen mice in group I were cured with NET (group IA) and 26 had persistent tumor (group IB). The 30 mice receiving saline (without tumor) were treated with either HIFU (group III, n = 15) or a sham procedure (group IV, n = 15). On day 26, all the animals received a second tumor challenge in the right flank. Reduced tumor growth following a second tumor challenge was demonstrated in group IA as compared with other groups (P < .001), implying a stimulation of host tumor immunity following curative HIFU treatment. The data suggest that HIFU may be an alternative modality for the treatment of unresectable neuroblastoma.

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