Effects of hyperthermia and membrane-active compounds or low ph on the membrane fluidity of chinese hamster ovary cells

J. R. Dynlacht, M. H. Fox

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Chinese hamster ovary (CHO) cells heated in the presence of the membrane-active agents procaine, ethanol, butylated hydroxytoluene (BHT) and glycerol were analysed for changes in fluorescence polarization of the lipid probe (1-[4(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene, an indicator of plasma membrane fluidity). Cells were heated in normal and acid (pH 6.6) medium. Procaine, ethanol, BHT and low pH sensitized cells to heat-killing. Procaine, ethanol and BHT decreased fluorescence polarization (increased plasma membrane fluidity) significantly. Polarization distributions for cells heated with these sensitizers were broadened substantially and were skewed toward lower polarization values. Glycerol, a heat-protector, inhibited changes in fluorescence polarization due to heating at temperatures up to 45.0°C. Heating cells at either 42 or 45°C in pH 6.6 medium had no significant effect on the fluorescence polarization compared with controls, while survival was reduced substantially. Thus, heat-sensitization of low pH cannot be ascribed to changes in membrane fluidity. The changes in membrane fluidity caused by other sensitizers and protector indicate that membrane fluidity changes may be a contributing cause of cell killing by hyperthermia.

Original languageEnglish (US)
Pages (from-to)351-362
Number of pages12
JournalInternational Journal of Hyperthermia
Volume8
Issue number3
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Keywords

  • Flow cytometry
  • Hyperthermia
  • Membrane fluidity
  • Sensitizers
  • TMA-DPH

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cancer Research

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