Effects of K+-stimulation and precursor loading on the in vivo release of dopamine, serotonin and their metabolites in the nucleus accumbens of the rat

X. M. Guan, W. J. McBride

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Abstract

The in vivo efflux of endogenous 3,4-dihydroxyphenylethylamine (DA) 5-hydroxytryptamine (5-HT), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the nucleus accumbens of the anesthetized rat was studied using a push-pull cannula. Local perfusion for 10 minutes with 35 mM K+ significantly (P<0.01) increased the release of DA and 5-HT, but not their metabolites, from their respective control levels of 0.95 and 0.04 pmol/15 min to 2.5 and 0.23 pmol/15 min. Exposure to 35 mM K+ a second and third time resulted in a decrement in the amount of stimulated release for both DA and 5-HT. This decrease was prevented by local perfusion for 10 minutes with 50 uM L-tyrosine and -tryptophan starting 30 minutes before each episode of depolarization. The baseline amounts of DOPAC, HVA and 5-HIAA observed in the perfusates were several fold higher than the basal levels found for 5-HT and Da. In the absence of precursors, the efflux of DOPAC, HVA and 5-HIAA decreased approximately 60, 40 and 25%, respectively, from the first to the last baseline fraction collected. Addition of precursors prevented the decrease for DOPAC and 5-HIAA but not for HVA. The data indicated that (a) the in vivo release of DA and 5-HT, along with their metabolites, could be simultaneously measured with the present procedure, and (b) when using the push-pull cannula, local perfusion with precursors may be necessary following periods of sustained and/or repeated stimulation in order to replenish the monoamine transmitter pools.

Original languageEnglish
Pages (from-to)2579-2586
Number of pages8
JournalLife Sciences
Volume40
Issue number26
DOIs
StatePublished - Jun 29 1987

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Nucleus Accumbens
Metabolites
Homovanillic Acid
Hydroxyindoleacetic Acid
Rats
Dopamine
Serotonin
Perfusion
Acids
3,4-Dihydroxyphenylacetic Acid
Level control
Depolarization
Tryptophan
Tyrosine
Transmitters
Cannula

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of K+-stimulation and precursor loading on the in vivo release of dopamine, serotonin and their metabolites in the nucleus accumbens of the rat. / Guan, X. M.; McBride, W. J.

In: Life Sciences, Vol. 40, No. 26, 29.06.1987, p. 2579-2586.

Research output: Contribution to journalArticle

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