Effects of lipopolysaccharide priming on acute ischemic brain injury

Shah Hinan Ahmed, Yong Y. He, Abdullah Nassief, Jian Xu, Xiao-Ming Xu, Chung Y. Hsu

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Background and Purpose - Infection has been implicated as a stroke risk factor. Activation and infiltration of polymorphonuclear neutrophils (PMNs) after cerebral ischemia may contribute to ischemic brain injury. This study was conducted to investigate how enhanced postischemic PMN infiltration by lipopolysaccharide (LPS) altered the acute ischemic outcomes. Methods - LPS (0.05 mg/kg SC) or vehicle was given to Long-Evans male rats 24 hours before ischemia. Focal cerebral ischemia was induced by temporary ligation of the right middle cerebral artery and both common carotid arteries for 45 minutes. Animals were killed 6 and 24 hours after reperfusion to determine the extent of PMN infiltration (myeloperoxidase assay), brain edema (wet-dry weight method), and vascular injury (fluorescein isothiocyanate-conjugated dextran extravasation). The infarct volumes were measured on the basis of TTC stain 24 hours after ischemia. Results - LPS had little effect on body temperature or peripheral white count but substantially enhanced PMN infiltration into the ischemic right middle cerebral artery cortex on the basis of myeloperoxidase activity (6 hours: control, 0 U/g; LPS, 0.186±0.025 U/g; 24 hours: control, 0.185±0.025 U/g; LPS, 0.290±0.040 U/g; P3; LPS, 108±12 mm3; P

Original languageEnglish (US)
Pages (from-to)193-199
Number of pages7
JournalStroke
Volume31
Issue number1
StatePublished - Jan 2000
Externally publishedYes

Fingerprint

Brain Injuries
Lipopolysaccharides
Neutrophil Infiltration
Middle Cerebral Artery
Brain Ischemia
Peroxidase
Ischemia
Long Evans Rats
Vascular System Injuries
Common Carotid Artery
Brain Edema
Body Temperature
Cerebral Cortex
Reperfusion
Ligation
Coloring Agents
Stroke
Weights and Measures
Infection

Keywords

  • Blood vessels
  • Brain edema
  • Cerebral ischemia
  • Inflammation
  • Neutrophils

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Ahmed, S. H., He, Y. Y., Nassief, A., Xu, J., Xu, X-M., & Hsu, C. Y. (2000). Effects of lipopolysaccharide priming on acute ischemic brain injury. Stroke, 31(1), 193-199.

Effects of lipopolysaccharide priming on acute ischemic brain injury. / Ahmed, Shah Hinan; He, Yong Y.; Nassief, Abdullah; Xu, Jian; Xu, Xiao-Ming; Hsu, Chung Y.

In: Stroke, Vol. 31, No. 1, 01.2000, p. 193-199.

Research output: Contribution to journalArticle

Ahmed, SH, He, YY, Nassief, A, Xu, J, Xu, X-M & Hsu, CY 2000, 'Effects of lipopolysaccharide priming on acute ischemic brain injury', Stroke, vol. 31, no. 1, pp. 193-199.
Ahmed SH, He YY, Nassief A, Xu J, Xu X-M, Hsu CY. Effects of lipopolysaccharide priming on acute ischemic brain injury. Stroke. 2000 Jan;31(1):193-199.
Ahmed, Shah Hinan ; He, Yong Y. ; Nassief, Abdullah ; Xu, Jian ; Xu, Xiao-Ming ; Hsu, Chung Y. / Effects of lipopolysaccharide priming on acute ischemic brain injury. In: Stroke. 2000 ; Vol. 31, No. 1. pp. 193-199.
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