Effects of Novel 3-dimensional Antibiotic-containing Electrospun Scaffolds on Dentin Discoloration

Margaret Louise A Porter, Eliseu A. Münchow, Maria T P Albuquerque, Kenneth Spolnik, Anderson Hara, Marco C. Bottino

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Introduction Although intracanal application of the triple antibiotic paste (TAP) may offer advantages (eg, disinfection), this practice has been associated with significant drawbacks, including tooth discoloration. In this study, the color change of dentin was monitored during treatment with distinct TAP pastes and novel tubular-shaped 3-dimensional electrospun scaffolds containing minocycline (MINO) or doxycycline (DOX). Methods Two TAP pastes (TAPMINO [MINO, metronidazole, and ciprofloxacin] and TAPDOX [DOX, metronidazole, and ciprofloxacin]), 4 scaffold-based groups containing MINO or DOX at distinct concentrations, 1 antibiotic-free scaffold, and 1 untreated group (control) were investigated. Human canines were sectioned at the cementoenamel junction and tubular-shaped scaffolds or paste were placed into the root canals and sealed. Color measurements (CIELab parameters) were performed at baseline and after 1, 3, 7, 14, 21, and 28 days. Color changes were expressed as ΔE values. In addition, scanning electron microscopy and energy-dispersive X-ray spectroscopy were also performed on the specimens after treatment. Data were analyzed using repeated measures analysis of variance (alpha = 0.05). Results All antibiotic-containing groups led to greater discoloration than the antibiotic-free groups. A severe discoloration occurred after 1 day. At the end of the experiment, antibiotic-treated samples exhibited crusts/agglomerates over the dentin surface, which totally or partially obliterated the dentinal tubules. The presence of MINO resulted in a greater color change than DOX. Conclusions Scaffolds containing MINO or DOX produced similar color change to dentin when compared with their respective TAP systems, although DOX-related discoloration was less pronounced.

Original languageEnglish (US)
Pages (from-to)106-112
Number of pages7
JournalJournal of Endodontics
Volume42
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Dentin
Ointments
Doxycycline
Minocycline
Anti-Bacterial Agents
Color
Metronidazole
Ciprofloxacin
Tooth Discoloration
Tooth Cervix
X-Ray Emission Spectrometry
Dental Pulp Cavity
Disinfection
Electron Scanning Microscopy
Canidae
Analysis of Variance
Control Groups

Keywords

  • Antibiotic
  • discoloration
  • disinfection
  • electrospinning
  • regeneration
  • scaffold

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effects of Novel 3-dimensional Antibiotic-containing Electrospun Scaffolds on Dentin Discoloration. / Porter, Margaret Louise A; Münchow, Eliseu A.; Albuquerque, Maria T P; Spolnik, Kenneth; Hara, Anderson; Bottino, Marco C.

In: Journal of Endodontics, Vol. 42, No. 1, 01.01.2016, p. 106-112.

Research output: Contribution to journalArticle

Porter, Margaret Louise A ; Münchow, Eliseu A. ; Albuquerque, Maria T P ; Spolnik, Kenneth ; Hara, Anderson ; Bottino, Marco C. / Effects of Novel 3-dimensional Antibiotic-containing Electrospun Scaffolds on Dentin Discoloration. In: Journal of Endodontics. 2016 ; Vol. 42, No. 1. pp. 106-112.
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AB - Introduction Although intracanal application of the triple antibiotic paste (TAP) may offer advantages (eg, disinfection), this practice has been associated with significant drawbacks, including tooth discoloration. In this study, the color change of dentin was monitored during treatment with distinct TAP pastes and novel tubular-shaped 3-dimensional electrospun scaffolds containing minocycline (MINO) or doxycycline (DOX). Methods Two TAP pastes (TAPMINO [MINO, metronidazole, and ciprofloxacin] and TAPDOX [DOX, metronidazole, and ciprofloxacin]), 4 scaffold-based groups containing MINO or DOX at distinct concentrations, 1 antibiotic-free scaffold, and 1 untreated group (control) were investigated. Human canines were sectioned at the cementoenamel junction and tubular-shaped scaffolds or paste were placed into the root canals and sealed. Color measurements (CIEL∗a∗b∗ parameters) were performed at baseline and after 1, 3, 7, 14, 21, and 28 days. Color changes were expressed as ΔE∗ values. In addition, scanning electron microscopy and energy-dispersive X-ray spectroscopy were also performed on the specimens after treatment. Data were analyzed using repeated measures analysis of variance (alpha = 0.05). Results All antibiotic-containing groups led to greater discoloration than the antibiotic-free groups. A severe discoloration occurred after 1 day. At the end of the experiment, antibiotic-treated samples exhibited crusts/agglomerates over the dentin surface, which totally or partially obliterated the dentinal tubules. The presence of MINO resulted in a greater color change than DOX. Conclusions Scaffolds containing MINO or DOX produced similar color change to dentin when compared with their respective TAP systems, although DOX-related discoloration was less pronounced.

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