Effects of obesity and stable weight reduction on UCP2 and UCP3 gene expression in humans

Antonio Vidal-Puig, Michael Rosenbaum, Robert C. Considine, Rudolph L. Leibel, G. Lynis Dohm, Bradford B. Lowell

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Objectives: The molecular determinants of energy expenditure are presently unknown. Recently, two uncoupling protein homologues, UCP2 and UCP3, have been identified. UCP2 is expressed widely, and UCP3 is expressed abundantly in skeletal muscle. Both could be important regulators of energy balance. In this paper, we investigated whether altered UCP2 and UCP3 mRNA levels are associated with obesity or weight reduction. Research Methods and Procedures: UCP2, UCP3 long and short mRNA levels were examined in skeletal muscle and in white adipose tissue of lean, obese, and weight-reduced individuals by RNase protection assay. Results: Expression of UCP2, UCP3S, and UCP3L mRNA in skeletal muscle was similar in lean individuals and in individuals with obesity at stable weight. In contrast, UCP3L and UCP3S mRNAs were decreased by 38% (p<0.0059) and 48% (p<0.0047), respectively, in 20% weight-reduced patients with obesity at stable weight. In contrast, UCP2 mRNA levels were increased by 30% in skeletal muscle of 20% weight-reduced subjects with obe-sity. In a different set of patients, mostly lean, UCP3L mRNA in skeletal muscle was decreased by 28% (p = 0.0425) after 10% weight reduction at stable weight. Expression of UCP2 mRNA in subcutaneous adipose tissue was similar in lean individuals and in individuals with obesity, and was increased by 58% during active weight loss. Discussion: Stabilization at reduced body weight in humans is associated with a decrease in UCP3 mRNA in muscle. It is possible that reduced UCP3 expression could contribute to decreased energy expenditure in weight-stable, weight-reduced individuals.

Original languageEnglish (US)
Pages (from-to)133-140
Number of pages8
JournalObesity Research
Volume7
Issue number2
DOIs
StatePublished - Jan 1 1999

Fingerprint

Weight Loss
obesity
weight loss
Obesity
Gene Expression
Weights and Measures
gene expression
Messenger RNA
skeletal muscle
Skeletal Muscle
energy expenditure
Energy Metabolism
White Adipose Tissue
white adipose tissue
Subcutaneous Fat
Ribonucleases
research methods
energy balance
adipose tissue
Body Weight

Keywords

  • Gene expression
  • Obesity
  • UCP
  • Uncoupling proteins
  • Weight reduction

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Public Health, Environmental and Occupational Health
  • Endocrinology
  • Food Science
  • Endocrinology, Diabetes and Metabolism

Cite this

Effects of obesity and stable weight reduction on UCP2 and UCP3 gene expression in humans. / Vidal-Puig, Antonio; Rosenbaum, Michael; Considine, Robert C.; Leibel, Rudolph L.; Dohm, G. Lynis; Lowell, Bradford B.

In: Obesity Research, Vol. 7, No. 2, 01.01.1999, p. 133-140.

Research output: Contribution to journalArticle

Vidal-Puig, Antonio ; Rosenbaum, Michael ; Considine, Robert C. ; Leibel, Rudolph L. ; Dohm, G. Lynis ; Lowell, Bradford B. / Effects of obesity and stable weight reduction on UCP2 and UCP3 gene expression in humans. In: Obesity Research. 1999 ; Vol. 7, No. 2. pp. 133-140.
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