Effects of octanoate and acetate upon hepatic glycolysis and lipogenesis

Nomura Takahide, Iguchi Akihisa, Sakamoto Nobuo, Robert A. Harris

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Octanoate and N6, O2'-dibutyryl adenosine 3',5'-monophosphate (dibutyryl cyclic AMP) cause a marked inhibition of net glucose utilization and lactate and pyruvate accumulation by hepatocytes isolated from meal-fed rats. Acetate is much less effective as an inhibitor of glycolysis. Fatty acid synthesis, as measured by tritiated water incorporation, is inhibited by dibutyryl cyclic AMP, whereas it is stimulated by 10 mM acetate and 1 mM octanoate. Stimulation of fatty acid synthesis by 1 mM octanoate, however, is lost paradoxically at higher concentrations of octanoate. Rates of fatty acid synthesis estimated by [1-14C]octanoate incorporation were consistently higher than rates calculated on the basis of tritiated water incorporation, raising the question as to which is the better index of the rate of de novo fatty acid synthesis. The effects of octanoate were studied because it was reasoned that this fatty acid should not inhibit acetyl-CoA carboxylase but should inhibit glycolysis and supply acetyl-CoA for lipogenesis. This was found to be the case, proving that glycolytic activity is not necessary for rapid rates of de novo fatty acid synthesis by liver.

Original languageEnglish (US)
Pages (from-to)315-320
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume754
Issue number3
DOIs
StatePublished - Dec 20 1983

Keywords

  • (Rat hepatocyte)
  • Acetate
  • Glycolysis
  • Lipogenesis
  • Octanoate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

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