Effects of parathyroid hormone on cAMP production and alkaline phosphatase activity in osteoblastic clone MC3T3-E1 cells

Yoshinori Nakatani, Mayuri Tsunoi, Yoshiuki Hakeda, Noriyoshi Kurihara, Kunya Fujita, Masayoshi Kumegawa

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The effect of parathyroid hormone (PTH) was tested on osteoblastic cells (clone MC3T3-El) cultured in serum-free medium containing 0.3% bovine serum albumin. PTH stimulated an increase in cAMP production in a dose-related fashion up to a concentration of 0.5 unit/ml, the maximum increase being 23-fold above that of controls. Although PTH did not affect DNA content, this peptide also increased dose-dependently alkaline phosphatase activity up to a concentration of 0.5 unit/ml. PTH plus isobutylmethyl xanthine elevated enzyme activity 1.5-fold over that in PTH-treated cells. These results strongly indicate that PTH has a direct stimulatory effect on the differentiation of osteoblasts via intracellular cAMP production in vitro.

Original languageEnglish (US)
Pages (from-to)894-906
Number of pages13
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Sep 28 1984


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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