Effects of parathyroid hormone on cAMP production and alkaline phosphatase activity in osteoblastic clone MC3T3-E1 cells

Yoshinori Nakatani, Mayuri Tsunoi, Yoshiuki Hakeda, Noriyoshi Kurihara, Kunya Fujita, Masayoshi Kumegawa

Research output: Contribution to journalArticle

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Abstract

The effect of parathyroid hormone (PTH) was tested on osteoblastic cells (clone MC3T3-El) cultured in serum-free medium containing 0.3% bovine serum albumin. PTH stimulated an increase in cAMP production in a dose-related fashion up to a concentration of 0.5 unit/ml, the maximum increase being 23-fold above that of controls. Although PTH did not affect DNA content, this peptide also increased dose-dependently alkaline phosphatase activity up to a concentration of 0.5 unit/ml. PTH plus isobutylmethyl xanthine elevated enzyme activity 1.5-fold over that in PTH-treated cells. These results strongly indicate that PTH has a direct stimulatory effect on the differentiation of osteoblasts via intracellular cAMP production in vitro.

Original languageEnglish (US)
Pages (from-to)894-906
Number of pages13
JournalBiochemical and Biophysical Research Communications
Volume123
Issue number3
DOIs
StatePublished - Sep 28 1984
Externally publishedYes

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Parathyroid Hormone
Alkaline Phosphatase
Clone Cells
Clone cells
Xanthine
Osteoblasts
Serum-Free Culture Media
Enzyme activity
Bovine Serum Albumin
Cells
Peptides
DNA
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effects of parathyroid hormone on cAMP production and alkaline phosphatase activity in osteoblastic clone MC3T3-E1 cells. / Nakatani, Yoshinori; Tsunoi, Mayuri; Hakeda, Yoshiuki; Kurihara, Noriyoshi; Fujita, Kunya; Kumegawa, Masayoshi.

In: Biochemical and Biophysical Research Communications, Vol. 123, No. 3, 28.09.1984, p. 894-906.

Research output: Contribution to journalArticle

Nakatani, Yoshinori ; Tsunoi, Mayuri ; Hakeda, Yoshiuki ; Kurihara, Noriyoshi ; Fujita, Kunya ; Kumegawa, Masayoshi. / Effects of parathyroid hormone on cAMP production and alkaline phosphatase activity in osteoblastic clone MC3T3-E1 cells. In: Biochemical and Biophysical Research Communications. 1984 ; Vol. 123, No. 3. pp. 894-906.
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