Effects of Phospholemman Expression on Swelling-Activated ion Currents and Volume Regulation in Embryonic Kidney Cells

Cristina E. Davis, Manoj K. Patel, James R. Miller, J. Edward John, Larry Jones, Amy L. Tucker, J. Paul Mounsey, J. Randall Moorman

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Phospholemman (PLM) is a 72-amino-acid phosphoprotein that is a major substrate for cAMP-dependent protein kinase, protein kinase C, and NIMA kinase. In lipid bilayers, PLM forms ion channels selective for Cl-, K +, and taurine. Effluxes of these abundant intracellular osmolytes play an important role in the control of dynamic cell volume changes in many cell types. We measured swelling-activated ion currents and regulatory volume decrease (RVD) in human embryonic kidney cells stably overexpressing canine cardiac PLM. In response to swelling, two clonal cell lines overexpressing PLM had increased swelling-activated ion current densities and faster and more extensive RVD. A third clonal cell line overexpressing mutant PLM showed reduced ion current densities and a diminished RVD response. These results suggest a role for PLM in the regulation of cell volume, perhaps as a modulator of an endogenous swelling-activated signal transduction pathway or possibly by participating directly in swelling-induced osmolyte efflux.

Original languageEnglish
Pages (from-to)177-187
Number of pages11
JournalNeurochemical Research
Volume29
Issue number1
DOIs
StatePublished - Jan 2004

Fingerprint

Swelling
Ions
Kidney
Cells
Cell Size
Current density
Cell Line
Signal transduction
Lipid bilayers
Phosphoproteins
Taurine
Lipid Bilayers
Cyclic AMP-Dependent Protein Kinases
Ion Channels
Protein Kinase C
Modulators
phospholemman
Canidae
Signal Transduction
Phosphotransferases

Keywords

  • Cell swelling
  • Ion channel
  • Phospholemman
  • Regulatory volume decrease

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Effects of Phospholemman Expression on Swelling-Activated ion Currents and Volume Regulation in Embryonic Kidney Cells. / Davis, Cristina E.; Patel, Manoj K.; Miller, James R.; John, J. Edward; Jones, Larry; Tucker, Amy L.; Mounsey, J. Paul; Moorman, J. Randall.

In: Neurochemical Research, Vol. 29, No. 1, 01.2004, p. 177-187.

Research output: Contribution to journalArticle

Davis, Cristina E. ; Patel, Manoj K. ; Miller, James R. ; John, J. Edward ; Jones, Larry ; Tucker, Amy L. ; Mounsey, J. Paul ; Moorman, J. Randall. / Effects of Phospholemman Expression on Swelling-Activated ion Currents and Volume Regulation in Embryonic Kidney Cells. In: Neurochemical Research. 2004 ; Vol. 29, No. 1. pp. 177-187.
@article{55a71bb02dfb42958a62066d320a074a,
title = "Effects of Phospholemman Expression on Swelling-Activated ion Currents and Volume Regulation in Embryonic Kidney Cells",
abstract = "Phospholemman (PLM) is a 72-amino-acid phosphoprotein that is a major substrate for cAMP-dependent protein kinase, protein kinase C, and NIMA kinase. In lipid bilayers, PLM forms ion channels selective for Cl-, K +, and taurine. Effluxes of these abundant intracellular osmolytes play an important role in the control of dynamic cell volume changes in many cell types. We measured swelling-activated ion currents and regulatory volume decrease (RVD) in human embryonic kidney cells stably overexpressing canine cardiac PLM. In response to swelling, two clonal cell lines overexpressing PLM had increased swelling-activated ion current densities and faster and more extensive RVD. A third clonal cell line overexpressing mutant PLM showed reduced ion current densities and a diminished RVD response. These results suggest a role for PLM in the regulation of cell volume, perhaps as a modulator of an endogenous swelling-activated signal transduction pathway or possibly by participating directly in swelling-induced osmolyte efflux.",
keywords = "Cell swelling, Ion channel, Phospholemman, Regulatory volume decrease",
author = "Davis, {Cristina E.} and Patel, {Manoj K.} and Miller, {James R.} and John, {J. Edward} and Larry Jones and Tucker, {Amy L.} and Mounsey, {J. Paul} and Moorman, {J. Randall}",
year = "2004",
month = "1",
doi = "10.1023/B:NERE.0000010447.24128.ac",
language = "English",
volume = "29",
pages = "177--187",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Effects of Phospholemman Expression on Swelling-Activated ion Currents and Volume Regulation in Embryonic Kidney Cells

AU - Davis, Cristina E.

AU - Patel, Manoj K.

AU - Miller, James R.

AU - John, J. Edward

AU - Jones, Larry

AU - Tucker, Amy L.

AU - Mounsey, J. Paul

AU - Moorman, J. Randall

PY - 2004/1

Y1 - 2004/1

N2 - Phospholemman (PLM) is a 72-amino-acid phosphoprotein that is a major substrate for cAMP-dependent protein kinase, protein kinase C, and NIMA kinase. In lipid bilayers, PLM forms ion channels selective for Cl-, K +, and taurine. Effluxes of these abundant intracellular osmolytes play an important role in the control of dynamic cell volume changes in many cell types. We measured swelling-activated ion currents and regulatory volume decrease (RVD) in human embryonic kidney cells stably overexpressing canine cardiac PLM. In response to swelling, two clonal cell lines overexpressing PLM had increased swelling-activated ion current densities and faster and more extensive RVD. A third clonal cell line overexpressing mutant PLM showed reduced ion current densities and a diminished RVD response. These results suggest a role for PLM in the regulation of cell volume, perhaps as a modulator of an endogenous swelling-activated signal transduction pathway or possibly by participating directly in swelling-induced osmolyte efflux.

AB - Phospholemman (PLM) is a 72-amino-acid phosphoprotein that is a major substrate for cAMP-dependent protein kinase, protein kinase C, and NIMA kinase. In lipid bilayers, PLM forms ion channels selective for Cl-, K +, and taurine. Effluxes of these abundant intracellular osmolytes play an important role in the control of dynamic cell volume changes in many cell types. We measured swelling-activated ion currents and regulatory volume decrease (RVD) in human embryonic kidney cells stably overexpressing canine cardiac PLM. In response to swelling, two clonal cell lines overexpressing PLM had increased swelling-activated ion current densities and faster and more extensive RVD. A third clonal cell line overexpressing mutant PLM showed reduced ion current densities and a diminished RVD response. These results suggest a role for PLM in the regulation of cell volume, perhaps as a modulator of an endogenous swelling-activated signal transduction pathway or possibly by participating directly in swelling-induced osmolyte efflux.

KW - Cell swelling

KW - Ion channel

KW - Phospholemman

KW - Regulatory volume decrease

UR - http://www.scopus.com/inward/record.url?scp=0842279580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0842279580&partnerID=8YFLogxK

U2 - 10.1023/B:NERE.0000010447.24128.ac

DO - 10.1023/B:NERE.0000010447.24128.ac

M3 - Article

C2 - 14992277

AN - SCOPUS:0842279580

VL - 29

SP - 177

EP - 187

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 1

ER -