Effects of platelet-activating factor on vascular permeability and granulocyte recruitment in the rat trachea

James J. Brokaw, Debra M. Prow, Carl M. Kirsch, Gary White

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Platelet-activating factor (PAF) is a phospholipid mediator known to produce several features of airway inflammation. We examined the effects of intravenous PAF on vascular permeability and granulocyte recruitment in the rat trachea. To assess vascular permeability, anesthetized rats were given injections of Evans blue dye (30 mg/kg, iv) and PAF (1-10 μg/kg, iv), and then their tracheas were removed and assayed spectrophotometrically for dye content. We found that a PAF dosage of 6 μg/kg increased the tracheal dye content 7-fold compared to controls. The amount of extravasated dye in the tracheas was significantly increased 1 min after PAF injection, was maximal at 5 min, and had returned to control levels by 10 min. To assess granulocyte recruitment, anesthetized rats were given an injection of PAF (6 μg/kg, iv), and then their tracheas were removed and stained to reveal myeloperoxidase-containing neutrophils and eosinophils. We found that the number of neutrophils in the tracheal mucosa was increased 7-fold from controls 5 min after PAF injection, but was not significantly increased 6 h later. The number of eosinophils in the tracheal mucosa was not significantly increased from controls at any time after PAF injection. We conclude that intravenous PAF causes a rapid but transient increase in vascular permeability in the rat trachea, and that intravenous PAF also causes a rapid but transient recruitment of neutrophils into the tracheal mucosa without a similar effect on eosinophils.

Original languageEnglish
Pages (from-to)109-119
Number of pages11
JournalLung
Volume169
Issue number1
DOIs
StatePublished - Dec 1991

Fingerprint

Platelet Activating Factor
Capillary Permeability
Trachea
Granulocytes
Coloring Agents
Injections
Eosinophils
Mucous Membrane
Neutrophils
Evans Blue
Neutrophil Infiltration
Peroxidase
Phospholipids
Inflammation

Keywords

  • Airway inflammation
  • Eosinophils
  • Neutrophils
  • Plasma extravasation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology

Cite this

Effects of platelet-activating factor on vascular permeability and granulocyte recruitment in the rat trachea. / Brokaw, James J.; Prow, Debra M.; Kirsch, Carl M.; White, Gary.

In: Lung, Vol. 169, No. 1, 12.1991, p. 109-119.

Research output: Contribution to journalArticle

Brokaw, James J. ; Prow, Debra M. ; Kirsch, Carl M. ; White, Gary. / Effects of platelet-activating factor on vascular permeability and granulocyte recruitment in the rat trachea. In: Lung. 1991 ; Vol. 169, No. 1. pp. 109-119.
@article{e6637e60332348fea5839006e0e33799,
title = "Effects of platelet-activating factor on vascular permeability and granulocyte recruitment in the rat trachea",
abstract = "Platelet-activating factor (PAF) is a phospholipid mediator known to produce several features of airway inflammation. We examined the effects of intravenous PAF on vascular permeability and granulocyte recruitment in the rat trachea. To assess vascular permeability, anesthetized rats were given injections of Evans blue dye (30 mg/kg, iv) and PAF (1-10 μg/kg, iv), and then their tracheas were removed and assayed spectrophotometrically for dye content. We found that a PAF dosage of 6 μg/kg increased the tracheal dye content 7-fold compared to controls. The amount of extravasated dye in the tracheas was significantly increased 1 min after PAF injection, was maximal at 5 min, and had returned to control levels by 10 min. To assess granulocyte recruitment, anesthetized rats were given an injection of PAF (6 μg/kg, iv), and then their tracheas were removed and stained to reveal myeloperoxidase-containing neutrophils and eosinophils. We found that the number of neutrophils in the tracheal mucosa was increased 7-fold from controls 5 min after PAF injection, but was not significantly increased 6 h later. The number of eosinophils in the tracheal mucosa was not significantly increased from controls at any time after PAF injection. We conclude that intravenous PAF causes a rapid but transient increase in vascular permeability in the rat trachea, and that intravenous PAF also causes a rapid but transient recruitment of neutrophils into the tracheal mucosa without a similar effect on eosinophils.",
keywords = "Airway inflammation, Eosinophils, Neutrophils, Plasma extravasation",
author = "Brokaw, {James J.} and Prow, {Debra M.} and Kirsch, {Carl M.} and Gary White",
year = "1991",
month = "12",
doi = "10.1007/BF02714148",
language = "English",
volume = "169",
pages = "109--119",
journal = "Lung",
issn = "0341-2040",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Effects of platelet-activating factor on vascular permeability and granulocyte recruitment in the rat trachea

AU - Brokaw, James J.

AU - Prow, Debra M.

AU - Kirsch, Carl M.

AU - White, Gary

PY - 1991/12

Y1 - 1991/12

N2 - Platelet-activating factor (PAF) is a phospholipid mediator known to produce several features of airway inflammation. We examined the effects of intravenous PAF on vascular permeability and granulocyte recruitment in the rat trachea. To assess vascular permeability, anesthetized rats were given injections of Evans blue dye (30 mg/kg, iv) and PAF (1-10 μg/kg, iv), and then their tracheas were removed and assayed spectrophotometrically for dye content. We found that a PAF dosage of 6 μg/kg increased the tracheal dye content 7-fold compared to controls. The amount of extravasated dye in the tracheas was significantly increased 1 min after PAF injection, was maximal at 5 min, and had returned to control levels by 10 min. To assess granulocyte recruitment, anesthetized rats were given an injection of PAF (6 μg/kg, iv), and then their tracheas were removed and stained to reveal myeloperoxidase-containing neutrophils and eosinophils. We found that the number of neutrophils in the tracheal mucosa was increased 7-fold from controls 5 min after PAF injection, but was not significantly increased 6 h later. The number of eosinophils in the tracheal mucosa was not significantly increased from controls at any time after PAF injection. We conclude that intravenous PAF causes a rapid but transient increase in vascular permeability in the rat trachea, and that intravenous PAF also causes a rapid but transient recruitment of neutrophils into the tracheal mucosa without a similar effect on eosinophils.

AB - Platelet-activating factor (PAF) is a phospholipid mediator known to produce several features of airway inflammation. We examined the effects of intravenous PAF on vascular permeability and granulocyte recruitment in the rat trachea. To assess vascular permeability, anesthetized rats were given injections of Evans blue dye (30 mg/kg, iv) and PAF (1-10 μg/kg, iv), and then their tracheas were removed and assayed spectrophotometrically for dye content. We found that a PAF dosage of 6 μg/kg increased the tracheal dye content 7-fold compared to controls. The amount of extravasated dye in the tracheas was significantly increased 1 min after PAF injection, was maximal at 5 min, and had returned to control levels by 10 min. To assess granulocyte recruitment, anesthetized rats were given an injection of PAF (6 μg/kg, iv), and then their tracheas were removed and stained to reveal myeloperoxidase-containing neutrophils and eosinophils. We found that the number of neutrophils in the tracheal mucosa was increased 7-fold from controls 5 min after PAF injection, but was not significantly increased 6 h later. The number of eosinophils in the tracheal mucosa was not significantly increased from controls at any time after PAF injection. We conclude that intravenous PAF causes a rapid but transient increase in vascular permeability in the rat trachea, and that intravenous PAF also causes a rapid but transient recruitment of neutrophils into the tracheal mucosa without a similar effect on eosinophils.

KW - Airway inflammation

KW - Eosinophils

KW - Neutrophils

KW - Plasma extravasation

UR - http://www.scopus.com/inward/record.url?scp=0025905641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025905641&partnerID=8YFLogxK

U2 - 10.1007/BF02714148

DO - 10.1007/BF02714148

M3 - Article

C2 - 1648156

AN - SCOPUS:0025905641

VL - 169

SP - 109

EP - 119

JO - Lung

JF - Lung

SN - 0341-2040

IS - 1

ER -