Effects of pycnogenol treatment on oxidative stress in streptozotocin-induced diabetic rats

A. Maritim, B. A. Dene, R. A. Sanders, John B. Watkins

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Free radicals and oxidative stress have been implicated in the etiology of diabetes and its complications. This in vivo study has examined whether subacute administration of pycnogenol, a French pine bark extract containing procyanidins that have strong antioxidant potential, alters biomarkers of oxidative stress in normal and diabetic rats. Diabetes was induced in female Sprague-Dawley rats by a single injection of streptozotocin (90 mg/kg body weight, ip), resulting (after 30 days) in subnormal body weight, increased serum glucose concentrations, and an increase in liver weight, liver/body weight ratios, total and glycated hemoglobin, and serum aspartate aminotransferase activity. Normal and diabetic rats were treated with pycnogenol (10 mg/kg body weight/day, ip) for 14 days. Pycnogenol treatment significantly reduced blood glucose concentrations in diabetic rats. Biochemical markers for oxidative stress were assessed in the liver, kidney, and heart. Elevated hepatic catalase activity in diabetic rats was restored to normal levels after pycnogenol treatment. Additionally, diabetic rats treated with pycnogenol had significantly elevated levels of reduced glutathione and glutathione redox enzyme activities. The results demonstrate that pycnogenol alters intracellular antioxidant defense mechanisms in streptozotocin-induced diabetic rats.

Original languageEnglish
Pages (from-to)193-199
Number of pages7
JournalJournal of Biochemical and Molecular Toxicology
Volume17
Issue number3
DOIs
StatePublished - 2003

Fingerprint

Oxidative stress
Streptozocin
Rats
Oxidative Stress
Body Weight
Liver
Glutathione
Medical problems
Therapeutics
Antioxidants
Biomarkers
Proanthocyanidins
Glycosylated Hemoglobin A
Diabetes Complications
Aspartate Aminotransferases
Serum
Catalase
Enzyme activity
Oxidation-Reduction
Free Radicals

Keywords

  • Antioxidant
  • Diabetes
  • Oxidative stress
  • Pycnogenol
  • Rat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Effects of pycnogenol treatment on oxidative stress in streptozotocin-induced diabetic rats. / Maritim, A.; Dene, B. A.; Sanders, R. A.; Watkins, John B.

In: Journal of Biochemical and Molecular Toxicology, Vol. 17, No. 3, 2003, p. 193-199.

Research output: Contribution to journalArticle

Maritim, A. ; Dene, B. A. ; Sanders, R. A. ; Watkins, John B. / Effects of pycnogenol treatment on oxidative stress in streptozotocin-induced diabetic rats. In: Journal of Biochemical and Molecular Toxicology. 2003 ; Vol. 17, No. 3. pp. 193-199.
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