Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors

Keith M. Stantz, Ning Cao, Bo Liu, Minsong Cao, Helen Chin-Sinex, Marc Mendonca, Jian Jian Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

Purpose: The purpose of this study is to monitor in vivo the IR dose dependent response of NF-κB and tumor hemodynamics as a function of time. Material and Methods: An MDA-231 breast cancer cell line was stably transfected with a firefly luciferase gene within the NF-kappaB promoter. Tumors on the right flank irradiated with a single fractionated dose of 5Gy or 10Gy. Over two weeks, photoacoustic spectroscopy (PCT-S), bioluminescence imaging (BLI), and dynamic contrast enhanced CT (DCE-CT) was used to monitor hemoglobin status, NF-kappaB expression, and physiology, respectively. Results: From the BLI, an increase in NF-kappaB expression was observed in both the right (irradiation) and left (nonirradiated) tumors, which peaked at 8-12 hours, returned to basal levels after 24 hours, and increased a second time from 3 to 7 days. This data identifies both a radiation-induced bystander effect and a bimodal longitudinal response associated with NF-κB-controlled luciferase promoter. The physiological results from DCE-CT measured an increase in perfusion (26%) two days after radiation and both a decrease in perfusion and an increase in fp by week 1 (10Gy cohort). PCT-S measured increased levels of oxygen saturation two days post IR, which did not change after 1 week. Initially, NF-κB would modify hemodynamics to increase oxygen delivery after IR insult. The secondary response appears to modulate tumor angiogenesis. Conclusions: A bimodal response to radiation was detected with NF-kappaB-controlled luciferase reporter with a concomitant hemodynamic response associated with tumor hypoxia. Experiments are being performed to increase statistics.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2010
DOIs
StatePublished - May 3 2010
EventPhotons Plus Ultrasound: Imaging and Sensing 2010 - San Francisco, CA, United States
Duration: Jan 24 2010Jan 26 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7564
ISSN (Print)1605-7422

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2010
CountryUnited States
CitySan Francisco, CA
Period1/24/101/26/10

Keywords

  • Breast cancer
  • DCE-CT
  • Ionizing radiation
  • NF-kappaB
  • Oxygen saturation
  • Photoacoustic computed tomographic spectroscopy
  • Physiology

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Stantz, K. M., Cao, N., Liu, B., Cao, M., Chin-Sinex, H., Mendonca, M., & Li, J. J. (2010). Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors. In Photons Plus Ultrasound: Imaging and Sensing 2010 [75641J] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7564). https://doi.org/10.1117/12.842238