Effects of Recombinant Human Tumor Necrosis Factor a, Recombinant Human γ-Interferon, and Prostaglandin E on Colony Formation of Human Hematopoietic Progenitor Cells Stimulated by Natural Human Pluripotent Colony-stimulating Factor, Pluripoietin a, and Recombinant Erythropoietin in Serum-free Cultures

Li Lu, Giao Hangoc, Edward Bruno, Ronald Hoffman, Hal E. Broxmeyer

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Abstract

The influences of pure human pluripotent colony-stimulating factor, highly purified pluripoietin a, pure recombinant human tumor necrosis factor a, pure recombinant human 7-interferon, and natural prostaglandin E 1 (PGE 1 ) were evaluated on colony formation of multipotential and erythroid progenitor cells in the presence of recombinant erythropoietin and hemin and on colony formation of granulocyte-macrophage progenitors in normal human marrow cultured in the presence or absence of serum. Serum was replaced by bovine serum albumin, iron-saturated transferrin, cholesterol, and calcium chloride. Increasing concentrations of pluripotent colony-stimulating factor and pluripoietin a stimulated increasing numbers of colonies from nonadherent low-density T-lymphocyte-depleted cells in the absence and presence of serum. Growth was usually greater in the presence of serum and on a unit basis pluripoietin a was more active than pluripotent colony-stimulating factor. Recombinant human tumor necrosis factor a and recombinant human 7-interferon suppressed colony formation colony forming unit-granulocyte-macrophage, burst forming unit-erythroid, and colony forming unit-granulocyteerythroid-macrophage-megakaryocyte; PGE 1 suppressed colony formation by colony-forming unit-granulocyte-macrophage, stimulated colony formation by burst forming unit-erythroid, and had no effects on colony formation by colony forming unit-granulocyte-erythroid-macrophage-megakaryocyte in both serum-containing and serum-free medium. The PGE 1 enhancing effects on erythroid colony formation required T-lymphocytes. Thus, results are similar using serum-containing and serum-free cultures of human bone marrow cells and serum-free defined culture medium can be used to study the mechanisms of action of purified natural and recombinant growth and suppressor molecules in vitro.

Original languageEnglish (US)
Pages (from-to)4357-4361
Number of pages5
JournalCancer Research
Volume46
Issue number9
StatePublished - Sep 1986

Fingerprint

Colony-Stimulating Factors
Erythropoietin
Hematopoietic Stem Cells
Prostaglandins E
Interferons
Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells
Serum
Serum-Free Culture Media
Myeloid Progenitor Cells
T-Lymphocytes
Calcium Chloride
Hemin
Megakaryocytes
Transferrin
Growth
Bovine Serum Albumin
human TNF protein
Bone Marrow Cells
Iron

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

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title = "Effects of Recombinant Human Tumor Necrosis Factor a, Recombinant Human γ-Interferon, and Prostaglandin E on Colony Formation of Human Hematopoietic Progenitor Cells Stimulated by Natural Human Pluripotent Colony-stimulating Factor, Pluripoietin a, and Recombinant Erythropoietin in Serum-free Cultures",
abstract = "The influences of pure human pluripotent colony-stimulating factor, highly purified pluripoietin a, pure recombinant human tumor necrosis factor a, pure recombinant human 7-interferon, and natural prostaglandin E 1 (PGE 1 ) were evaluated on colony formation of multipotential and erythroid progenitor cells in the presence of recombinant erythropoietin and hemin and on colony formation of granulocyte-macrophage progenitors in normal human marrow cultured in the presence or absence of serum. Serum was replaced by bovine serum albumin, iron-saturated transferrin, cholesterol, and calcium chloride. Increasing concentrations of pluripotent colony-stimulating factor and pluripoietin a stimulated increasing numbers of colonies from nonadherent low-density T-lymphocyte-depleted cells in the absence and presence of serum. Growth was usually greater in the presence of serum and on a unit basis pluripoietin a was more active than pluripotent colony-stimulating factor. Recombinant human tumor necrosis factor a and recombinant human 7-interferon suppressed colony formation colony forming unit-granulocyte-macrophage, burst forming unit-erythroid, and colony forming unit-granulocyteerythroid-macrophage-megakaryocyte; PGE 1 suppressed colony formation by colony-forming unit-granulocyte-macrophage, stimulated colony formation by burst forming unit-erythroid, and had no effects on colony formation by colony forming unit-granulocyte-erythroid-macrophage-megakaryocyte in both serum-containing and serum-free medium. The PGE 1 enhancing effects on erythroid colony formation required T-lymphocytes. Thus, results are similar using serum-containing and serum-free cultures of human bone marrow cells and serum-free defined culture medium can be used to study the mechanisms of action of purified natural and recombinant growth and suppressor molecules in vitro.",
author = "Li Lu and Giao Hangoc and Edward Bruno and Ronald Hoffman and Broxmeyer, {Hal E.}",
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