Effects of resolvin d1 on cell survival and cytokine expression of human gingival fibroblasts

Mohamed Khaled, Nouf Al Shibani, Nawaf Labban, Ghada Batarseh, Fengyu Song, John Ruby, L. Windsor

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Tissue breakdown in periodontitis is initiated by bacteria, such as Porphyromonas gingivalis, and is caused largely by host responses. Resolvins protect the host against acute inflammation by blocking the migration of polymorphonuclear neutrophils to initiate resolution. The effects of resolvins on human gingival fibroblasts (HGFs) are unknown. This study examines the effects of resolvin D1 on HGF survival and cytokine expression when treated with or without P. gingivalis supernatant. Methods: Cytotoxicity of resolvin D1 on HGFs with or without a toxic level of P. gingivalis supernatant was measured with lactate dehydrogenase assays. Cytokine arrays were performed on HGF-conditioned media treated with or without resolvin D1 and with or without P. gingivalis supernatant. Results: Resolvin D1 had no cytotoxic effects on HGFs at concentrations between 1 and 1,000 nM (all P <0.05). Resolvin D1 (1,000 nM) significantly inhibited the toxic effects of 13.5% (v/v) P. gingivalis supernatant on HGFs (P = 0.002). Resolvin D1 significantly reduced the expression of interleukin (IL)-6 (P = 0.010) and monocyte chemoattractant protein (MCP)-1 (P = 0.04) in untreated fibroblasts. P. gingivalis (10%) supernatant significantly increased the expression levels of granulocyte-macrophage colony-stimulating factor (CSF), granulocyte CSF, growth-regulated oncogene (GRO), IL-5, IL-6, IL-7, IL-8, IL-10, MCP-1, MCP-2, MCP-3, and monokine induced by g-interferon. Resolvin D1 significantly reduced the expression of GRO (P = 0.04), marginally reduced the levels of MCP-1 (P = 0.10), and marginally increased the levels of transforming growth factor (TGF)-b1 (P = 0.07) from HGFs treated with P. gingivalis supernatant. Conclusions: Resolvin D1 altered the cytotoxicity of P. gingivalis supernatant on HGFs. Resolvin D1 significantly reduced GRO, marginally reduced MCP-1, and marginally increased TGF-b1 from P. gingivalis-treated HGFs, which could alter the ability of P. gingivalis to induce inflammation.

Original languageEnglish (US)
Pages (from-to)1838-1846
Number of pages9
JournalJournal of Periodontology
Volume84
Issue number12
DOIs
StatePublished - Dec 2013

Fingerprint

Porphyromonas gingivalis
Cell Survival
Fibroblasts
Cytokines
Chemokine CCL2
Oncogenes
Poisons
Transforming Growth Factors
Chemokine CCL8
Interleukin-6
Chemokine CCL7
Growth
resolvin D1
Monokines
Inflammation
Interleukin-7
Periodontitis
Interleukin-5
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor

Keywords

  • Bacteria
  • Cell biology
  • Cytokines
  • Fibroblasts
  • Microbiology

ASJC Scopus subject areas

  • Periodontics

Cite this

Effects of resolvin d1 on cell survival and cytokine expression of human gingival fibroblasts. / Khaled, Mohamed; Shibani, Nouf Al; Labban, Nawaf; Batarseh, Ghada; Song, Fengyu; Ruby, John; Windsor, L.

In: Journal of Periodontology, Vol. 84, No. 12, 12.2013, p. 1838-1846.

Research output: Contribution to journalArticle

Khaled, Mohamed ; Shibani, Nouf Al ; Labban, Nawaf ; Batarseh, Ghada ; Song, Fengyu ; Ruby, John ; Windsor, L. / Effects of resolvin d1 on cell survival and cytokine expression of human gingival fibroblasts. In: Journal of Periodontology. 2013 ; Vol. 84, No. 12. pp. 1838-1846.
@article{93d5132761fc4d5897e6ac080e278bf3,
title = "Effects of resolvin d1 on cell survival and cytokine expression of human gingival fibroblasts",
abstract = "Background: Tissue breakdown in periodontitis is initiated by bacteria, such as Porphyromonas gingivalis, and is caused largely by host responses. Resolvins protect the host against acute inflammation by blocking the migration of polymorphonuclear neutrophils to initiate resolution. The effects of resolvins on human gingival fibroblasts (HGFs) are unknown. This study examines the effects of resolvin D1 on HGF survival and cytokine expression when treated with or without P. gingivalis supernatant. Methods: Cytotoxicity of resolvin D1 on HGFs with or without a toxic level of P. gingivalis supernatant was measured with lactate dehydrogenase assays. Cytokine arrays were performed on HGF-conditioned media treated with or without resolvin D1 and with or without P. gingivalis supernatant. Results: Resolvin D1 had no cytotoxic effects on HGFs at concentrations between 1 and 1,000 nM (all P <0.05). Resolvin D1 (1,000 nM) significantly inhibited the toxic effects of 13.5{\%} (v/v) P. gingivalis supernatant on HGFs (P = 0.002). Resolvin D1 significantly reduced the expression of interleukin (IL)-6 (P = 0.010) and monocyte chemoattractant protein (MCP)-1 (P = 0.04) in untreated fibroblasts. P. gingivalis (10{\%}) supernatant significantly increased the expression levels of granulocyte-macrophage colony-stimulating factor (CSF), granulocyte CSF, growth-regulated oncogene (GRO), IL-5, IL-6, IL-7, IL-8, IL-10, MCP-1, MCP-2, MCP-3, and monokine induced by g-interferon. Resolvin D1 significantly reduced the expression of GRO (P = 0.04), marginally reduced the levels of MCP-1 (P = 0.10), and marginally increased the levels of transforming growth factor (TGF)-b1 (P = 0.07) from HGFs treated with P. gingivalis supernatant. Conclusions: Resolvin D1 altered the cytotoxicity of P. gingivalis supernatant on HGFs. Resolvin D1 significantly reduced GRO, marginally reduced MCP-1, and marginally increased TGF-b1 from P. gingivalis-treated HGFs, which could alter the ability of P. gingivalis to induce inflammation.",
keywords = "Bacteria, Cell biology, Cytokines, Fibroblasts, Microbiology",
author = "Mohamed Khaled and Shibani, {Nouf Al} and Nawaf Labban and Ghada Batarseh and Fengyu Song and John Ruby and L. Windsor",
year = "2013",
month = "12",
doi = "10.1902/jop.2013.120388",
language = "English (US)",
volume = "84",
pages = "1838--1846",
journal = "Journal of Periodontology",
issn = "0022-3492",
publisher = "American Academy of Periodontology",
number = "12",

}

TY - JOUR

T1 - Effects of resolvin d1 on cell survival and cytokine expression of human gingival fibroblasts

AU - Khaled, Mohamed

AU - Shibani, Nouf Al

AU - Labban, Nawaf

AU - Batarseh, Ghada

AU - Song, Fengyu

AU - Ruby, John

AU - Windsor, L.

PY - 2013/12

Y1 - 2013/12

N2 - Background: Tissue breakdown in periodontitis is initiated by bacteria, such as Porphyromonas gingivalis, and is caused largely by host responses. Resolvins protect the host against acute inflammation by blocking the migration of polymorphonuclear neutrophils to initiate resolution. The effects of resolvins on human gingival fibroblasts (HGFs) are unknown. This study examines the effects of resolvin D1 on HGF survival and cytokine expression when treated with or without P. gingivalis supernatant. Methods: Cytotoxicity of resolvin D1 on HGFs with or without a toxic level of P. gingivalis supernatant was measured with lactate dehydrogenase assays. Cytokine arrays were performed on HGF-conditioned media treated with or without resolvin D1 and with or without P. gingivalis supernatant. Results: Resolvin D1 had no cytotoxic effects on HGFs at concentrations between 1 and 1,000 nM (all P <0.05). Resolvin D1 (1,000 nM) significantly inhibited the toxic effects of 13.5% (v/v) P. gingivalis supernatant on HGFs (P = 0.002). Resolvin D1 significantly reduced the expression of interleukin (IL)-6 (P = 0.010) and monocyte chemoattractant protein (MCP)-1 (P = 0.04) in untreated fibroblasts. P. gingivalis (10%) supernatant significantly increased the expression levels of granulocyte-macrophage colony-stimulating factor (CSF), granulocyte CSF, growth-regulated oncogene (GRO), IL-5, IL-6, IL-7, IL-8, IL-10, MCP-1, MCP-2, MCP-3, and monokine induced by g-interferon. Resolvin D1 significantly reduced the expression of GRO (P = 0.04), marginally reduced the levels of MCP-1 (P = 0.10), and marginally increased the levels of transforming growth factor (TGF)-b1 (P = 0.07) from HGFs treated with P. gingivalis supernatant. Conclusions: Resolvin D1 altered the cytotoxicity of P. gingivalis supernatant on HGFs. Resolvin D1 significantly reduced GRO, marginally reduced MCP-1, and marginally increased TGF-b1 from P. gingivalis-treated HGFs, which could alter the ability of P. gingivalis to induce inflammation.

AB - Background: Tissue breakdown in periodontitis is initiated by bacteria, such as Porphyromonas gingivalis, and is caused largely by host responses. Resolvins protect the host against acute inflammation by blocking the migration of polymorphonuclear neutrophils to initiate resolution. The effects of resolvins on human gingival fibroblasts (HGFs) are unknown. This study examines the effects of resolvin D1 on HGF survival and cytokine expression when treated with or without P. gingivalis supernatant. Methods: Cytotoxicity of resolvin D1 on HGFs with or without a toxic level of P. gingivalis supernatant was measured with lactate dehydrogenase assays. Cytokine arrays were performed on HGF-conditioned media treated with or without resolvin D1 and with or without P. gingivalis supernatant. Results: Resolvin D1 had no cytotoxic effects on HGFs at concentrations between 1 and 1,000 nM (all P <0.05). Resolvin D1 (1,000 nM) significantly inhibited the toxic effects of 13.5% (v/v) P. gingivalis supernatant on HGFs (P = 0.002). Resolvin D1 significantly reduced the expression of interleukin (IL)-6 (P = 0.010) and monocyte chemoattractant protein (MCP)-1 (P = 0.04) in untreated fibroblasts. P. gingivalis (10%) supernatant significantly increased the expression levels of granulocyte-macrophage colony-stimulating factor (CSF), granulocyte CSF, growth-regulated oncogene (GRO), IL-5, IL-6, IL-7, IL-8, IL-10, MCP-1, MCP-2, MCP-3, and monokine induced by g-interferon. Resolvin D1 significantly reduced the expression of GRO (P = 0.04), marginally reduced the levels of MCP-1 (P = 0.10), and marginally increased the levels of transforming growth factor (TGF)-b1 (P = 0.07) from HGFs treated with P. gingivalis supernatant. Conclusions: Resolvin D1 altered the cytotoxicity of P. gingivalis supernatant on HGFs. Resolvin D1 significantly reduced GRO, marginally reduced MCP-1, and marginally increased TGF-b1 from P. gingivalis-treated HGFs, which could alter the ability of P. gingivalis to induce inflammation.

KW - Bacteria

KW - Cell biology

KW - Cytokines

KW - Fibroblasts

KW - Microbiology

UR - http://www.scopus.com/inward/record.url?scp=84890908156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890908156&partnerID=8YFLogxK

U2 - 10.1902/jop.2013.120388

DO - 10.1902/jop.2013.120388

M3 - Article

C2 - 23398023

AN - SCOPUS:84890908156

VL - 84

SP - 1838

EP - 1846

JO - Journal of Periodontology

JF - Journal of Periodontology

SN - 0022-3492

IS - 12

ER -