Effects of shortened erythropoietin exposure on sheep marrow cultures

G. David Roodman, J. M. Kaplan, M. E. Kaplan, E. D. Zanjani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To determine whether erythropoietin (Ep) is required only to trigger, or also to sustain the growth of erythroid progenitor (CFU-E) in vitro, the effect of limiting the time of exposure of sheep bone marrow CFU-E to Ep on erythroid colony (EC) formation was examined. Restricting the in vitro exposure of CFU-E to 2-24 h, either by brief preincubation with Ep or by neutralization of Ep present in culture with anti-Ep, resulted in a 70-90% reduction of EC formation, and the colonies that were produced were of a small size. In contrast, no inhibition of EC size or number occurred when CFU-E were exposed to Ep for more than 24 h. These results suggest that Ep also modulates the proliferative events subsequent to the initial triggering of CFU-E which lead to the formation of erythrocytes.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalBritish Journal of Haematology
Volume47
Issue number2
StatePublished - 1981
Externally publishedYes

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Erythropoietin
Erythroid Precursor Cells
Sheep
Bone Marrow
Erythrocytes
Growth

ASJC Scopus subject areas

  • Hematology

Cite this

Roodman, G. D., Kaplan, J. M., Kaplan, M. E., & Zanjani, E. D. (1981). Effects of shortened erythropoietin exposure on sheep marrow cultures. British Journal of Haematology, 47(2), 195-201.

Effects of shortened erythropoietin exposure on sheep marrow cultures. / Roodman, G. David; Kaplan, J. M.; Kaplan, M. E.; Zanjani, E. D.

In: British Journal of Haematology, Vol. 47, No. 2, 1981, p. 195-201.

Research output: Contribution to journalArticle

Roodman, GD, Kaplan, JM, Kaplan, ME & Zanjani, ED 1981, 'Effects of shortened erythropoietin exposure on sheep marrow cultures', British Journal of Haematology, vol. 47, no. 2, pp. 195-201.
Roodman, G. David ; Kaplan, J. M. ; Kaplan, M. E. ; Zanjani, E. D. / Effects of shortened erythropoietin exposure on sheep marrow cultures. In: British Journal of Haematology. 1981 ; Vol. 47, No. 2. pp. 195-201.
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