Effects of the combination treatment of raloxifene and alendronate on the biomechanical properties of vertebral bone

Tamim Diab, Jason Wang, Susan Reinwald, Robert E. Guldberg, David Burr

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Raloxifene (RAL) and alendronate (ALN) improve the biomechanical properties of bone by different mechanisms. The goal here was to investigate the effects of combination treatment of RAL and ALN on the biomechanical properties of vertebral bone. Six-month-old Sprague-Dawley rats (n=80) were randomized into five experimental groups (sham, OVX, OVX+RAL, OVX+ALN, and OVX+RAL+ALN; n=16/group). Following euthanization, structural and derived material biomechanical properties of vertebral bodies were assessed. Density and dynamic histomorphometric measurements were made on cancellous bone. The results demonstrate that the structural biomechanical properties of vertebral bone are improved with the combination treatment. Stiffness and ultimate load of the OVX+RAL and OVX+ALN groups were significantly lower than those of sham animals, but the combination treatment with RAL+ALN was not significantly different from sham. Furthermore, the OVX+RAL+ALN group was the only agent-treated group in which the ultimate load was significantly higher than that in OVX animals (p<.05). Cancellous bone fractional volume (BV/TVcanc) and bone mineral density (aBMD) also were improved with the combination treatment. BV/TVcanc of the OVX+RAL+ALN group was 6.7% and 8.7% greater than that of the OVX+RAL (p<.05) and OVX+ALN (p<.05) groups, respectively. Areal BMD of the OVX+RAL or OVX+ALN groups was not significantly different from that in OVX animals, but the value in animals undergoing combination treatment was significantly higher than that in OVX or OVX+RAL animals alone and not significantly different from that in sham-operated animals. Turnover rates of both the RAL+ALN and ALN alone groups were lower than in the RAL-treated alone group (p<.05). We conclude that the combination treatment of raloxifene and alendronate has beneficial effects on bone volume, resulting in improvement in the structural properties of vertebral bone.

Original languageEnglish
Pages (from-to)270-276
Number of pages7
JournalJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Volume26
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

Alendronate
Bone and Bones
Raloxifene Hydrochloride
Bone Density

Keywords

  • ALENDRONATE
  • BIOMECHANICAL PROPERTIES
  • DENSITY
  • FRACTURE
  • RALOXIFENE

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Effects of the combination treatment of raloxifene and alendronate on the biomechanical properties of vertebral bone. / Diab, Tamim; Wang, Jason; Reinwald, Susan; Guldberg, Robert E.; Burr, David.

In: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 26, No. 2, 02.2011, p. 270-276.

Research output: Contribution to journalArticle

@article{4101dbd0fd434894bdfb4646ccf34a33,
title = "Effects of the combination treatment of raloxifene and alendronate on the biomechanical properties of vertebral bone",
abstract = "Raloxifene (RAL) and alendronate (ALN) improve the biomechanical properties of bone by different mechanisms. The goal here was to investigate the effects of combination treatment of RAL and ALN on the biomechanical properties of vertebral bone. Six-month-old Sprague-Dawley rats (n=80) were randomized into five experimental groups (sham, OVX, OVX+RAL, OVX+ALN, and OVX+RAL+ALN; n=16/group). Following euthanization, structural and derived material biomechanical properties of vertebral bodies were assessed. Density and dynamic histomorphometric measurements were made on cancellous bone. The results demonstrate that the structural biomechanical properties of vertebral bone are improved with the combination treatment. Stiffness and ultimate load of the OVX+RAL and OVX+ALN groups were significantly lower than those of sham animals, but the combination treatment with RAL+ALN was not significantly different from sham. Furthermore, the OVX+RAL+ALN group was the only agent-treated group in which the ultimate load was significantly higher than that in OVX animals (p<.05). Cancellous bone fractional volume (BV/TVcanc) and bone mineral density (aBMD) also were improved with the combination treatment. BV/TVcanc of the OVX+RAL+ALN group was 6.7{\%} and 8.7{\%} greater than that of the OVX+RAL (p<.05) and OVX+ALN (p<.05) groups, respectively. Areal BMD of the OVX+RAL or OVX+ALN groups was not significantly different from that in OVX animals, but the value in animals undergoing combination treatment was significantly higher than that in OVX or OVX+RAL animals alone and not significantly different from that in sham-operated animals. Turnover rates of both the RAL+ALN and ALN alone groups were lower than in the RAL-treated alone group (p<.05). We conclude that the combination treatment of raloxifene and alendronate has beneficial effects on bone volume, resulting in improvement in the structural properties of vertebral bone.",
keywords = "ALENDRONATE, BIOMECHANICAL PROPERTIES, DENSITY, FRACTURE, RALOXIFENE",
author = "Tamim Diab and Jason Wang and Susan Reinwald and Guldberg, {Robert E.} and David Burr",
year = "2011",
month = "2",
doi = "10.1002/jbmr.197",
language = "English",
volume = "26",
pages = "270--276",
journal = "Journal of Bone and Mineral Research",
issn = "0884-0431",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Effects of the combination treatment of raloxifene and alendronate on the biomechanical properties of vertebral bone

AU - Diab, Tamim

AU - Wang, Jason

AU - Reinwald, Susan

AU - Guldberg, Robert E.

AU - Burr, David

PY - 2011/2

Y1 - 2011/2

N2 - Raloxifene (RAL) and alendronate (ALN) improve the biomechanical properties of bone by different mechanisms. The goal here was to investigate the effects of combination treatment of RAL and ALN on the biomechanical properties of vertebral bone. Six-month-old Sprague-Dawley rats (n=80) were randomized into five experimental groups (sham, OVX, OVX+RAL, OVX+ALN, and OVX+RAL+ALN; n=16/group). Following euthanization, structural and derived material biomechanical properties of vertebral bodies were assessed. Density and dynamic histomorphometric measurements were made on cancellous bone. The results demonstrate that the structural biomechanical properties of vertebral bone are improved with the combination treatment. Stiffness and ultimate load of the OVX+RAL and OVX+ALN groups were significantly lower than those of sham animals, but the combination treatment with RAL+ALN was not significantly different from sham. Furthermore, the OVX+RAL+ALN group was the only agent-treated group in which the ultimate load was significantly higher than that in OVX animals (p<.05). Cancellous bone fractional volume (BV/TVcanc) and bone mineral density (aBMD) also were improved with the combination treatment. BV/TVcanc of the OVX+RAL+ALN group was 6.7% and 8.7% greater than that of the OVX+RAL (p<.05) and OVX+ALN (p<.05) groups, respectively. Areal BMD of the OVX+RAL or OVX+ALN groups was not significantly different from that in OVX animals, but the value in animals undergoing combination treatment was significantly higher than that in OVX or OVX+RAL animals alone and not significantly different from that in sham-operated animals. Turnover rates of both the RAL+ALN and ALN alone groups were lower than in the RAL-treated alone group (p<.05). We conclude that the combination treatment of raloxifene and alendronate has beneficial effects on bone volume, resulting in improvement in the structural properties of vertebral bone.

AB - Raloxifene (RAL) and alendronate (ALN) improve the biomechanical properties of bone by different mechanisms. The goal here was to investigate the effects of combination treatment of RAL and ALN on the biomechanical properties of vertebral bone. Six-month-old Sprague-Dawley rats (n=80) were randomized into five experimental groups (sham, OVX, OVX+RAL, OVX+ALN, and OVX+RAL+ALN; n=16/group). Following euthanization, structural and derived material biomechanical properties of vertebral bodies were assessed. Density and dynamic histomorphometric measurements were made on cancellous bone. The results demonstrate that the structural biomechanical properties of vertebral bone are improved with the combination treatment. Stiffness and ultimate load of the OVX+RAL and OVX+ALN groups were significantly lower than those of sham animals, but the combination treatment with RAL+ALN was not significantly different from sham. Furthermore, the OVX+RAL+ALN group was the only agent-treated group in which the ultimate load was significantly higher than that in OVX animals (p<.05). Cancellous bone fractional volume (BV/TVcanc) and bone mineral density (aBMD) also were improved with the combination treatment. BV/TVcanc of the OVX+RAL+ALN group was 6.7% and 8.7% greater than that of the OVX+RAL (p<.05) and OVX+ALN (p<.05) groups, respectively. Areal BMD of the OVX+RAL or OVX+ALN groups was not significantly different from that in OVX animals, but the value in animals undergoing combination treatment was significantly higher than that in OVX or OVX+RAL animals alone and not significantly different from that in sham-operated animals. Turnover rates of both the RAL+ALN and ALN alone groups were lower than in the RAL-treated alone group (p<.05). We conclude that the combination treatment of raloxifene and alendronate has beneficial effects on bone volume, resulting in improvement in the structural properties of vertebral bone.

KW - ALENDRONATE

KW - BIOMECHANICAL PROPERTIES

KW - DENSITY

KW - FRACTURE

KW - RALOXIFENE

UR - http://www.scopus.com/inward/record.url?scp=79251509585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251509585&partnerID=8YFLogxK

U2 - 10.1002/jbmr.197

DO - 10.1002/jbmr.197

M3 - Article

VL - 26

SP - 270

EP - 276

JO - Journal of Bone and Mineral Research

JF - Journal of Bone and Mineral Research

SN - 0884-0431

IS - 2

ER -