Effects of tobacco and P. gingivalis on gingival fibroblasts

W. Zhang, F. Song, L. Windsor

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cigarette smoke condensate (CSC) is the particulate matter of cigarette smoke. Porphyromonas gingivalis (P. gingivalis) is an opportunistic pathogen involved in periodontitis. It was hypothesized that the combination of CSC and P. gingivalis would increase the collagen-degrading ability of human gingival fibroblasts (HGFs). In this study, HGFs were exposed to CSC, P. gingivalis supernatant, and CSC plus P. gingivalis supernatant. The collagen-degrading ability and protein/mRNA levels of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) of HGFs were examined. The combined treatment increased collagen degradation, protein levels of active forms of MMP-1, MMP-2, MMP-3, and MMP-14 in conditioned media, and the low-molecular-weight fragment of MMP-14 in membrane extracts, as well as mRNA levels of MMP-1, MMP-2, and MMP-14. In conclusion, the combined effects of CSC and P. gingivalis increased HGF-mediated collagen degradation by destroying the balance between MMPs and TIMPs at multiple levels.

Original languageEnglish (US)
Pages (from-to)527-531
Number of pages5
JournalJournal of Dental Research
Volume89
Issue number5
DOIs
StatePublished - May 2010

Fingerprint

Porphyromonas gingivalis
Smoke
Tobacco Products
Tobacco
Fibroblasts
Matrix Metalloproteinase 14
Collagen
Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase 1
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinase 2
Matrix Metalloproteinase 3
Messenger RNA
Particulate Matter
Periodontitis
Conditioned Culture Medium
Proteolysis
Molecular Weight
Membranes
Proteins

Keywords

  • Cigarette smoke condensate
  • Collagen degradation
  • Gingival fibroblasts
  • Matrix metalloproteinases
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effects of tobacco and P. gingivalis on gingival fibroblasts. / Zhang, W.; Song, F.; Windsor, L.

In: Journal of Dental Research, Vol. 89, No. 5, 05.2010, p. 527-531.

Research output: Contribution to journalArticle

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