Effects of tobacco on cytokine expression from human endothelial cells

E. Allam, K. Delacruz, A. Ghoneima, J. Sun, L. Windsor

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: To investigate the effects of nicotine and cigarette smoke condensate (CSC) exposure on cytokine expression from human endothelial cells in order to identify one possible mechanism that smoking plays in the pathogenesis of both periodontal disease (PDD) and cardiovascular disease (CVD). Methods: Human endothelial cells (HUVECs) were exposed to different concentrations of nicotine and CSC to examine the effects that they have on cell proliferation and cytotoxicity. Non-toxic levels were then used to examine cytokine expression using cytokine protein arrays. Results: Exposure to nicotine caused significant down-regulation in the expression of IL-10 (P = 0.046), growth-regulated oncogene (GRO)α (P = 0.036), MCP-1 (P = 0.046), and GMCSF (P = 0.004) compared with the control untreated HUVECs. Exposure to CSC caused significant down-regulation in the expression of GRO (P = 0.04), GROα (P = 0.01), IL-6 (P = 0.03), and MCP-1 (P = 0.04) compared with the control untreated HUVECs. Conclusions: Exposure of HUVECs to nicotine or CSC affects the levels of cytokine expression including reduction in anti-inflammatory and chemoattractant cytokines. This may subsequently affect the host defensive mechanisms of the tissues. The action of toxic chemicals in tobacco smoke on endothelial cells is a potential pathogenic mechanism that may in part explain the association between tobacco, PDD, and CVD.

Original languageEnglish (US)
Pages (from-to)660-665
Number of pages6
JournalOral Diseases
Volume19
Issue number7
DOIs
StatePublished - 2013

Fingerprint

Smoke
Tobacco
Nicotine
Endothelial Cells
Tobacco Products
Cytokines
Oncogenes
Periodontal Diseases
Cardiovascular Diseases
Down-Regulation
Growth
Pharmacologic Actions
Toxic Actions
Protein Array Analysis
Chemotactic Factors
Interleukin-10
Interleukin-6
Anti-Inflammatory Agents
Smoking
Cell Proliferation

Keywords

  • Cytokines
  • Endothelial cells
  • Tobacco

ASJC Scopus subject areas

  • Dentistry(all)
  • Otorhinolaryngology
  • Medicine(all)

Cite this

Effects of tobacco on cytokine expression from human endothelial cells. / Allam, E.; Delacruz, K.; Ghoneima, A.; Sun, J.; Windsor, L.

In: Oral Diseases, Vol. 19, No. 7, 2013, p. 660-665.

Research output: Contribution to journalArticle

Allam, E. ; Delacruz, K. ; Ghoneima, A. ; Sun, J. ; Windsor, L. / Effects of tobacco on cytokine expression from human endothelial cells. In: Oral Diseases. 2013 ; Vol. 19, No. 7. pp. 660-665.
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N2 - Objective: To investigate the effects of nicotine and cigarette smoke condensate (CSC) exposure on cytokine expression from human endothelial cells in order to identify one possible mechanism that smoking plays in the pathogenesis of both periodontal disease (PDD) and cardiovascular disease (CVD). Methods: Human endothelial cells (HUVECs) were exposed to different concentrations of nicotine and CSC to examine the effects that they have on cell proliferation and cytotoxicity. Non-toxic levels were then used to examine cytokine expression using cytokine protein arrays. Results: Exposure to nicotine caused significant down-regulation in the expression of IL-10 (P = 0.046), growth-regulated oncogene (GRO)α (P = 0.036), MCP-1 (P = 0.046), and GMCSF (P = 0.004) compared with the control untreated HUVECs. Exposure to CSC caused significant down-regulation in the expression of GRO (P = 0.04), GROα (P = 0.01), IL-6 (P = 0.03), and MCP-1 (P = 0.04) compared with the control untreated HUVECs. Conclusions: Exposure of HUVECs to nicotine or CSC affects the levels of cytokine expression including reduction in anti-inflammatory and chemoattractant cytokines. This may subsequently affect the host defensive mechanisms of the tissues. The action of toxic chemicals in tobacco smoke on endothelial cells is a potential pathogenic mechanism that may in part explain the association between tobacco, PDD, and CVD.

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