Effects of virally expressed interleukin-10 on vaccinia virus infection in mice

M. G. Kurilla, S. Swaminathan, R. M. Welsh, E. Kieff, R. R. Brutkiewicz

Research output: Contribution to journalComment/debate

48 Scopus citations

Abstract

To investigate the in vivo role of interleukin-10 (IL-10) in viral infection, we compared infections with a recombinant vaccinia virus (VV) expressing IL-10 (VV-IL10) under control of the VV P7.5 promoter and a control virus (VV-βgal) in normal and severe combined immunodeficient mice. In normal mice, VV-IL10 infection resulted in less natural killer cell activity at 3 days postinfection and less VV-specific cytotoxic T-cell activity at 6 or 7 days postinfection than VV-βgal infection. However, the use of dermal scarification or intraperitoneal, intranasal, or intracerebral inoculation into immunocompetent mice resulted in no difference between VV- IL10 and VV-βgal in visible lesions, mortality, protective immunity to a 100-fold lethal VV challenge, or VV-specific antibody response. In the immunodeficient mice, VV-IL10 infection resulted in greater natural killer cell activity and lower virus replication than VV-βgal infection. These in vivo effects were subtler and more complex than had been anticipated. From the VV-IL10 murine model, the Epstein-Barr virus-encoded homolog of human IL- 10, BCRF1, may provide a selective advantage by blunting the early human natural killer cell and cytotoxic T-cell responses so that Epstein-Barr virus can establish a well-contained latent infection in B lymphocytes.

Original languageEnglish (US)
Pages (from-to)7623-7628
Number of pages6
JournalJournal of virology
Volume67
Issue number12
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Effects of virally expressed interleukin-10 on vaccinia virus infection in mice'. Together they form a unique fingerprint.

  • Cite this