Efficient NES-dependent protein nuclear export requires ongoing synthesis and export of mRNAs

Heather M. O'Hagan, Mats Ljungman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The mechanisms regulating nuclear export of proteins are not fully understood. To investigate whether the efficiency of protein nuclear export may depend on ongoing RNA synthesis and/or mRNA nuclear export, we used a microinjection approach with a fluorescent reporter protein containing a nuclear export signal (NES) and scored protein export in human fibroblasts under conditions when the synthesis or export of mRNAs was inhibited. We show that inhibition of transcription significantly attenuated generic NES-dependent nuclear export. Furthermore, digestion of endogenous nuclear RNAs by co-microinjection of RNAse A inhibited NES-dependent nuclear export. Finally, nuclear export of the NES reporter protein was significantly inhibited in cells in which nuclear export of mRNAs had been specifically blocked by microinjection of anti-TAP antibodies or by expression of a dominant negative form of NUP160. These results demonstrate a novel role for ongoing synthesis and export of mRNAs in NES-dependent protein nuclear export.

Original languageEnglish (US)
Pages (from-to)548-559
Number of pages12
JournalExperimental Cell Research
Volume297
Issue number2
DOIs
StatePublished - Jul 15 2004
Externally publishedYes

Keywords

  • CRM1
  • NES-GFP reporter protein
  • NUP160
  • Nuclear microinjection
  • Protein nuclear export
  • TAP
  • Transcription
  • mRNA nuclear export

ASJC Scopus subject areas

  • Cell Biology

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