EGFL6 regulates the asymmetric division, maintenance, and metastasis of ALDH+ ovarian cancer cells

Shoumei Bai, Patrick Ingram, Yu Chih Chen, Ning Deng, Alex Pearson, Yashar Niknafs, Patrick O'Hayer, Yun Wang, Zhong-Yin Zhang, Elisa Boscolo, Joyce Bischoff, Euisik Yoon, Ronald J. Buckanovich

Research output: Contribution to journalArticle

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Abstract

Little is known about the factors that regulate the asymmetric division of cancer stem-like cells (CSC).Here,we demonstrate that EGFL6, a stem cell regulatory factor expressed in ovarian tumor cells and vasculature, regulates ALDH+ ovarian CSC. EGFL6 signaled at least in part via the oncoprotein SHP2 with concomitant activation of ERK. EGFL6 signaling promoted the migration and asymmetric division of ALDH+ ovarian CSC. As such, EGFL6 increased not only tumor growth but also metastasis. Silencing of EGFL6 or SHP2 limited numbers of ALDH+ cells and reduced tumor growth, supporting a critical role for EGFL6/SHP2 in ALDH+ cell maintenance. Notably, systemic administration of an EGFL6-neutralizing antibody we generated restricted tumor growth and metastasis, specifically blocking ovarian cancer cell recruitment to the ovary. Together, our results offer a preclinical proof of concept for EGFL6 as a novel therapeutic target for the treatment of ovarian cancer. Cancer Res; 76(21); 6396-409.

Original languageEnglish (US)
Pages (from-to)6396-6409
Number of pages14
JournalCancer Research
Volume76
Issue number21
DOIs
StatePublished - Nov 1 2016

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Ovarian Neoplasms
Maintenance
Neoplastic Stem Cells
Neoplasm Metastasis
Neoplasms
Growth
Stem Cell Factor
Oncogene Proteins
Neutralizing Antibodies
Ovary
Cell Count
Therapeutics

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Bai, S., Ingram, P., Chen, Y. C., Deng, N., Pearson, A., Niknafs, Y., ... Buckanovich, R. J. (2016). EGFL6 regulates the asymmetric division, maintenance, and metastasis of ALDH+ ovarian cancer cells. Cancer Research, 76(21), 6396-6409. https://doi.org/10.1158/0008-5472.CAN-16-0225

EGFL6 regulates the asymmetric division, maintenance, and metastasis of ALDH+ ovarian cancer cells. / Bai, Shoumei; Ingram, Patrick; Chen, Yu Chih; Deng, Ning; Pearson, Alex; Niknafs, Yashar; O'Hayer, Patrick; Wang, Yun; Zhang, Zhong-Yin; Boscolo, Elisa; Bischoff, Joyce; Yoon, Euisik; Buckanovich, Ronald J.

In: Cancer Research, Vol. 76, No. 21, 01.11.2016, p. 6396-6409.

Research output: Contribution to journalArticle

Bai, S, Ingram, P, Chen, YC, Deng, N, Pearson, A, Niknafs, Y, O'Hayer, P, Wang, Y, Zhang, Z-Y, Boscolo, E, Bischoff, J, Yoon, E & Buckanovich, RJ 2016, 'EGFL6 regulates the asymmetric division, maintenance, and metastasis of ALDH+ ovarian cancer cells', Cancer Research, vol. 76, no. 21, pp. 6396-6409. https://doi.org/10.1158/0008-5472.CAN-16-0225
Bai, Shoumei ; Ingram, Patrick ; Chen, Yu Chih ; Deng, Ning ; Pearson, Alex ; Niknafs, Yashar ; O'Hayer, Patrick ; Wang, Yun ; Zhang, Zhong-Yin ; Boscolo, Elisa ; Bischoff, Joyce ; Yoon, Euisik ; Buckanovich, Ronald J. / EGFL6 regulates the asymmetric division, maintenance, and metastasis of ALDH+ ovarian cancer cells. In: Cancer Research. 2016 ; Vol. 76, No. 21. pp. 6396-6409.
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