Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells

Tatiana E. Salazar, Matthew R. Richardson, Eleni Beli, Matthew S. Ripsch, John George, Youngsook Kim, Yaqian Duan, Leni Moldovan, Yuanqing Yan, Ashay Bhatwadekar, Vaishnavi Jadhav, Jared A. Smith, Susan McGorray, Alicia L. Bertone, Dmitry Traktuev, Keith L. March, Luis M. Colon-Perez, Keith G. Avin, Emily Sims, Julie A. Mund & 24 others Jamie Case, Xiaolin Deng, Min Su Kim, Bruce McDavitt, Michael E. Boulton, Jeffrey Thinschmidt, Sergio Li Calzi, Stephanie D. Fitz, Robyn K. Fuchs, Stuart J. Warden, Todd McKinley, Anantha Shekhar, Marcelo Febo, Philip Johnson, Lung Ji Chang, Zhanguo Gao, Mikhail G. Kolonin, Song Lai, Jingfeng Ma, Xinzhong Dong, Fletcher White, Huisheng Xie, Mervin Yoder, Maria B. Grant

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Electroacupuncture (EA) performed in rats and humans using limb acupuncture sites, LI-4 and LI-11, and GV-14 and GV-20 (humans) and Bai-hui (rats) increased functional connectivity between the anterior hypothalamus and the amygdala and mobilized mesenchymal stem cells (MSCs) into the systemic circulation. In human subjects, the source of the MSC was found to be primarily adipose tissue, whereas in rodents the tissue sources were considered more heterogeneous. Pharmacological disinhibition of rat hypothalamus enhanced sympathetic nervous system (SNS) activation and similarly resulted in a release of MSC into the circulation. EA-mediated SNS activation was further supported by browning of white adipose tissue in rats. EA treatment of rats undergoing partial rupture of the Achilles tendon resulted in reduced mechanical hyperalgesia, increased serum interleukin-10 levels and tendon remodeling, effects blocked in propranolol-treated rodents. To distinguish the afferent role of the peripheral nervous system, phosphoinositide-interacting regulator of transient receptor potential channels (Pirt)-GCaMP3 (genetically encoded calcium sensor) mice were treated with EA acupuncture points, ST-36 and LIV-3, and GV-14 and Bai-hui and resulted in a rapid activation of primary sensory neurons. EA activated sensory ganglia and SNS centers to mediate the release of MSC that can enhance tissue repair, increase anti-inflammatory cytokine production and provide pronounced analgesic relief. Stem Cells 2017;35:1303–1315.

Original languageEnglish (US)
Pages (from-to)1303-1315
Number of pages13
JournalStem Cells
Volume35
Issue number5
DOIs
StatePublished - May 1 2017

Fingerprint

Electroacupuncture
Mesenchymal Stromal Cells
Central Nervous System
Sympathetic Nervous System
Rodentia
Sensory Ganglia
Transient Receptor Potential Channels
Anterior Hypothalamus
White Adipose Tissue
Acupuncture Points
Achilles Tendon
Hyperalgesia
Peripheral Nervous System
Acupuncture
Sensory Receptor Cells
Phosphatidylinositols
Amygdala
Propranolol
Tendons
Interleukin-10

Keywords

  • Adult stem cells
  • Mesenchymal stem cells
  • Nervous system
  • Neurones

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Salazar, T. E., Richardson, M. R., Beli, E., Ripsch, M. S., George, J., Kim, Y., ... Grant, M. B. (2017). Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells. Stem Cells, 35(5), 1303-1315. https://doi.org/10.1002/stem.2613

Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells. / Salazar, Tatiana E.; Richardson, Matthew R.; Beli, Eleni; Ripsch, Matthew S.; George, John; Kim, Youngsook; Duan, Yaqian; Moldovan, Leni; Yan, Yuanqing; Bhatwadekar, Ashay; Jadhav, Vaishnavi; Smith, Jared A.; McGorray, Susan; Bertone, Alicia L.; Traktuev, Dmitry; March, Keith L.; Colon-Perez, Luis M.; Avin, Keith G.; Sims, Emily; Mund, Julie A.; Case, Jamie; Deng, Xiaolin; Kim, Min Su; McDavitt, Bruce; Boulton, Michael E.; Thinschmidt, Jeffrey; Li Calzi, Sergio; Fitz, Stephanie D.; Fuchs, Robyn K.; Warden, Stuart J.; McKinley, Todd; Shekhar, Anantha; Febo, Marcelo; Johnson, Philip; Chang, Lung Ji; Gao, Zhanguo; Kolonin, Mikhail G.; Lai, Song; Ma, Jingfeng; Dong, Xinzhong; White, Fletcher; Xie, Huisheng; Yoder, Mervin; Grant, Maria B.

In: Stem Cells, Vol. 35, No. 5, 01.05.2017, p. 1303-1315.

Research output: Contribution to journalArticle

Salazar, TE, Richardson, MR, Beli, E, Ripsch, MS, George, J, Kim, Y, Duan, Y, Moldovan, L, Yan, Y, Bhatwadekar, A, Jadhav, V, Smith, JA, McGorray, S, Bertone, AL, Traktuev, D, March, KL, Colon-Perez, LM, Avin, KG, Sims, E, Mund, JA, Case, J, Deng, X, Kim, MS, McDavitt, B, Boulton, ME, Thinschmidt, J, Li Calzi, S, Fitz, SD, Fuchs, RK, Warden, SJ, McKinley, T, Shekhar, A, Febo, M, Johnson, P, Chang, LJ, Gao, Z, Kolonin, MG, Lai, S, Ma, J, Dong, X, White, F, Xie, H, Yoder, M & Grant, MB 2017, 'Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells', Stem Cells, vol. 35, no. 5, pp. 1303-1315. https://doi.org/10.1002/stem.2613
Salazar TE, Richardson MR, Beli E, Ripsch MS, George J, Kim Y et al. Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells. Stem Cells. 2017 May 1;35(5):1303-1315. https://doi.org/10.1002/stem.2613
Salazar, Tatiana E. ; Richardson, Matthew R. ; Beli, Eleni ; Ripsch, Matthew S. ; George, John ; Kim, Youngsook ; Duan, Yaqian ; Moldovan, Leni ; Yan, Yuanqing ; Bhatwadekar, Ashay ; Jadhav, Vaishnavi ; Smith, Jared A. ; McGorray, Susan ; Bertone, Alicia L. ; Traktuev, Dmitry ; March, Keith L. ; Colon-Perez, Luis M. ; Avin, Keith G. ; Sims, Emily ; Mund, Julie A. ; Case, Jamie ; Deng, Xiaolin ; Kim, Min Su ; McDavitt, Bruce ; Boulton, Michael E. ; Thinschmidt, Jeffrey ; Li Calzi, Sergio ; Fitz, Stephanie D. ; Fuchs, Robyn K. ; Warden, Stuart J. ; McKinley, Todd ; Shekhar, Anantha ; Febo, Marcelo ; Johnson, Philip ; Chang, Lung Ji ; Gao, Zhanguo ; Kolonin, Mikhail G. ; Lai, Song ; Ma, Jingfeng ; Dong, Xinzhong ; White, Fletcher ; Xie, Huisheng ; Yoder, Mervin ; Grant, Maria B. / Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells. In: Stem Cells. 2017 ; Vol. 35, No. 5. pp. 1303-1315.
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AU - George, John

AU - Kim, Youngsook

AU - Duan, Yaqian

AU - Moldovan, Leni

AU - Yan, Yuanqing

AU - Bhatwadekar, Ashay

AU - Jadhav, Vaishnavi

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AU - McGorray, Susan

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AU - Traktuev, Dmitry

AU - March, Keith L.

AU - Colon-Perez, Luis M.

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AU - Sims, Emily

AU - Mund, Julie A.

AU - Case, Jamie

AU - Deng, Xiaolin

AU - Kim, Min Su

AU - McDavitt, Bruce

AU - Boulton, Michael E.

AU - Thinschmidt, Jeffrey

AU - Li Calzi, Sergio

AU - Fitz, Stephanie D.

AU - Fuchs, Robyn K.

AU - Warden, Stuart J.

AU - McKinley, Todd

AU - Shekhar, Anantha

AU - Febo, Marcelo

AU - Johnson, Philip

AU - Chang, Lung Ji

AU - Gao, Zhanguo

AU - Kolonin, Mikhail G.

AU - Lai, Song

AU - Ma, Jingfeng

AU - Dong, Xinzhong

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