Electron microscopic analysis of a spherical mitochondrial structure

Wen Xing Ding, Min Li, Joanna M. Biazik, David G. Morgan, Fengli Guo, Hong Min Ni, Michael Goheen, Eeva Liisa Eskelinen, Xiao Ming Yin

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Mitochondria undergo dynamic structural alterations to meet changing needs and to maintain homeostasis. We report here a novel mitochondrial structure. Conventional transmission electron microscopic examination of murine embryonic fibroblasts treated with carbonyl cyanide m-chlorophenylhydrazone (CCCP), a mitochondrial uncoupler, found that more than half of the mitochondria presented a ring-shaped or C-shaped morphology. Many of these mitochondria seemed to have engulfed various cytosolic components. Serial sections through individual mitochondria indicated that they formed a ball-like structure with an internal lumen surrounded by the membranes and containing cytosolic materials. Notably, the lumen was connected to the external cytoplasm through a small opening. Electron tomographic reconstruction of the mitochondrial spheroids demonstrated the membrane topology and confirmed the vesicular configuration of this mitochondrial structure. The outside periphery and the lumen were defined by the outer membranes, which were lined with the inner membranes. Matrix and cristae were retained but distributed unevenly with less being kept near the luminal opening. Mitochondrial spheroids seem to form in response to oxidative mitochondrial damage independently of mitophagy. The structural features of the mitochondrial spheroids thus represent a novel mitochondrial dynamics.

Original languageEnglish (US)
Pages (from-to)42373-42378
Number of pages6
JournalJournal of Biological Chemistry
Volume287
Issue number50
DOIs
StatePublished - Dec 7 2012

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Mitochondria
Electrons
Membranes
Mitochondrial Degradation
Mitochondrial Dynamics
Structural dynamics
Fibroblasts
Microscopic examination
Cytoplasm
Homeostasis
Topology

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ding, W. X., Li, M., Biazik, J. M., Morgan, D. G., Guo, F., Ni, H. M., ... Yin, X. M. (2012). Electron microscopic analysis of a spherical mitochondrial structure. Journal of Biological Chemistry, 287(50), 42373-42378. https://doi.org/10.1074/jbc.M112.413674

Electron microscopic analysis of a spherical mitochondrial structure. / Ding, Wen Xing; Li, Min; Biazik, Joanna M.; Morgan, David G.; Guo, Fengli; Ni, Hong Min; Goheen, Michael; Eskelinen, Eeva Liisa; Yin, Xiao Ming.

In: Journal of Biological Chemistry, Vol. 287, No. 50, 07.12.2012, p. 42373-42378.

Research output: Contribution to journalArticle

Ding, WX, Li, M, Biazik, JM, Morgan, DG, Guo, F, Ni, HM, Goheen, M, Eskelinen, EL & Yin, XM 2012, 'Electron microscopic analysis of a spherical mitochondrial structure', Journal of Biological Chemistry, vol. 287, no. 50, pp. 42373-42378. https://doi.org/10.1074/jbc.M112.413674
Ding WX, Li M, Biazik JM, Morgan DG, Guo F, Ni HM et al. Electron microscopic analysis of a spherical mitochondrial structure. Journal of Biological Chemistry. 2012 Dec 7;287(50):42373-42378. https://doi.org/10.1074/jbc.M112.413674
Ding, Wen Xing ; Li, Min ; Biazik, Joanna M. ; Morgan, David G. ; Guo, Fengli ; Ni, Hong Min ; Goheen, Michael ; Eskelinen, Eeva Liisa ; Yin, Xiao Ming. / Electron microscopic analysis of a spherical mitochondrial structure. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 50. pp. 42373-42378.
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