Electron microscopic demonstration of horseradish peroxidase-tetramethylbenzidine reaction product as a method for identifying sensory nerve fibers in the rat tooth pulp

Dennis F. Turner, Carl Marfurt

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The purpose of the present investigation was to determine if the horseradish peroxidase could be used as a method for labeling sensory nerve fibers (specifically, tooth pulp afferents) for detailed ultrastructural analyses. HRP injected into the trigeminal ganglion of adult rats was taken up by ganglion cell bodies and transported anterogradely to their peripheral endings in the dental tissues. Following perfusion-fixation, the teeth were decalcified in EDTA, sectioned, reacted for HRP activity according to the tetramethylbenzidine (TMB) technique, and processed for electron microscopy. The HRP-TMB reaction product was clearly visible within most of the axons in the dental pulp, appearing as conspicuous, rectangular shaped aggregates of fine rods or needles.

Original languageEnglish (US)
Pages (from-to)213-217
Number of pages5
JournalNeuroscience Letters
Volume41
Issue number3
DOIs
StatePublished - Nov 11 1983
Externally publishedYes

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Horseradish Peroxidase
Nerve Fibers
Tooth
Electrons
Dental Pulp
Trigeminal Ganglion
Edetic Acid
Ganglia
Needles
Axons
Electron Microscopy
Perfusion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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abstract = "The purpose of the present investigation was to determine if the horseradish peroxidase could be used as a method for labeling sensory nerve fibers (specifically, tooth pulp afferents) for detailed ultrastructural analyses. HRP injected into the trigeminal ganglion of adult rats was taken up by ganglion cell bodies and transported anterogradely to their peripheral endings in the dental tissues. Following perfusion-fixation, the teeth were decalcified in EDTA, sectioned, reacted for HRP activity according to the tetramethylbenzidine (TMB) technique, and processed for electron microscopy. The HRP-TMB reaction product was clearly visible within most of the axons in the dental pulp, appearing as conspicuous, rectangular shaped aggregates of fine rods or needles.",
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