Electrospray ionization mass spectrometry analysis of lysophospholipids in human ascitic fluids

Comparison of the lysophospholipid contents in malignant vs nonmalignant ascitic fluids

Yi Jin Xiao, Benjamin Schwartz, Monique Washington, Alexander Kennedy, Kenneth Webster, Jerome Belinson, Yan Xu

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

Lysophospholipids (lyso-PLs), including various glycerol-based and sphingosine-based lysophospholipids, play important roles in many biochemical, physiological, and pathological processes. The classical methods to analyze these lipids involve gas chromatography and/or high-performance liquid chromatography, which are time-consuming, cumbersome, and sometimes inaccurate due to the incomplete separation of closely related lipid species. We now describe the quantitative analysis of lyso-PLs in ascites samples from patients with ovarian cancer using electrospray ionization spectrometry. Three new classes of lyso-PL molecules are detected: alkyl-LPA, alkenyl-LPA, and methylated lysophosphatidylethanolamine. Importantly, the following lysophospholipid species are significantly increased in ascites from patients with ovarian cancer, compared to patients with nonmalignant diseases (e.g., liver failure): LPA (including acyl-, alkyl-, and alkenyl-LPA species), lysophosphatidylinositol, and sphingosylphosphorylcholine. Lysophosphorylcholine contents are also significantly different among ascitic fluids from the two groups of patients. However, the total phosphate content in ascites samples from patients with ovarian cancer is not significantly different compared to that from patients with nonmalignant disease.

Original languageEnglish (US)
Pages (from-to)302-313
Number of pages12
JournalAnalytical Biochemistry
Volume290
Issue number2
DOIs
StatePublished - Mar 15 2001
Externally publishedYes

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Lysophospholipids
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Ascitic Fluid
Mass spectrometry
Fluids
Ascites
Ovarian Neoplasms
Lipids
Sphingosine
High performance liquid chromatography
Gas chromatography
Liver
Glycerol
Spectrometry
Biochemical Phenomena
Phosphates
Physiological Phenomena
Molecules
Liver Failure

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Electrospray ionization mass spectrometry analysis of lysophospholipids in human ascitic fluids : Comparison of the lysophospholipid contents in malignant vs nonmalignant ascitic fluids. / Xiao, Yi Jin; Schwartz, Benjamin; Washington, Monique; Kennedy, Alexander; Webster, Kenneth; Belinson, Jerome; Xu, Yan.

In: Analytical Biochemistry, Vol. 290, No. 2, 15.03.2001, p. 302-313.

Research output: Contribution to journalArticle

Xiao, Yi Jin ; Schwartz, Benjamin ; Washington, Monique ; Kennedy, Alexander ; Webster, Kenneth ; Belinson, Jerome ; Xu, Yan. / Electrospray ionization mass spectrometry analysis of lysophospholipids in human ascitic fluids : Comparison of the lysophospholipid contents in malignant vs nonmalignant ascitic fluids. In: Analytical Biochemistry. 2001 ; Vol. 290, No. 2. pp. 302-313.
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