Eliminating xenoantigen expression on swine rbc

Zheng Yu Wang, Gregory R. Martens, Ross L. Blankenship, Richard A. Sidner, Ping Li, Jose L. Estrada, Matthew Tector, A. Joseph Tector

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Background. The rapidly improving tools of genetic engineering maymake it possible to overcome the humoral immune barrier that prevents xenotransplantation. We hypothesize that levels of human antibody binding to donor tissues from swine must approximate the antibody binding occurring in allotransplantation. It is uncertain if this is an attainable goal. Here we perform an initial analysis of this issue by comparing human antibody binding to red blood cells (RBC) isolated from knockout swine and to allogeneic or autologous human RBC.Methods. Human sera were incubated with RBC isolated from various genetically engineered swine or from humans. The level of IgG and IgM binding to these cells were compared using either flow cytometry or a novelmass spectrometric assay. Results.Mass spectroscopic quantitation of human antibody binding demonstrated that as few as 3 gene inactivations can reduce the levels human antibody binding to swine RBC that is as low as autologous human RBC. Flow cytometry showed that RBC from 2-gene knockout swine exhibited less human antibody binding than human blood group O allogeneic RBC in 22% of tested sera. Deletion of a third gene from pigs resulted in 30% of human samples having less IgG and IgM RBC xenoreactivity than alloreactivity. Conclusions. Xenoantigenicity of swine RBC can be eliminated via gene disruption. These results suggest that the gene knockout approach may be able reduce antigenicity in other pig tissues to levels that enable the xenotransplantation humoral barrier to be overcome.

Original languageEnglish (US)
Pages (from-to)517-523
Number of pages7
JournalTransplantation
Volume101
Issue number3
DOIs
StatePublished - Jan 1 2017

ASJC Scopus subject areas

  • Transplantation

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  • Cite this

    Wang, Z. Y., Martens, G. R., Blankenship, R. L., Sidner, R. A., Li, P., Estrada, J. L., Tector, M., & Tector, A. J. (2017). Eliminating xenoantigen expression on swine rbc. Transplantation, 101(3), 517-523. https://doi.org/10.1097/TP.0000000000001302