Embryonic cerebellar graft development during acute phase of gliosis in the cerebellum of pcd mutant mice.

A. C. Chang, B. Ghetti

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Anatomical and biochemical changes in cerebellum after the onset of pcd gene defects have revealed in addition to Purkinje cell degeneration, the occurrence of secondary degenerative events in granule neurons, neurons of the inferior olivary complex and deep cerebellar nuclei. Transplantation in young mutant animals thereby presents a prospect that not only the missing Purkinje cells may be replaced but the subsequent transneuronal losses may be minimized. The present study has evaluated the outcome of implanting embryonic cerebellar cell suspensions into pcd mutants at the age of 45 day-old, a stage marked by the end of Purkinje cell degeneration. Immunocytochemistry (ICC) was used to monitor the extent of gliosis in host cerebellum and to trace the development of embryonic Purkinje cells grafted. The results suggest that the donor cells have no apparent difficulty in survival during reactive gliosis. Migration of these neuroblasts from the grafted zone into adjacent host molecular layer and significant expression of calcium binding protein (CaBP) by grafted Purkinje cells were noted within 9 days after transplantation. Dendritic patterns of grafted Purkinje cells are well-developed by day 17 post transplantation up to 3 months. In addition, synaptic contacts between host fiber endings and the CaBP-positive dendritic spines are present in the molecular layer of folia containing the graft, suggesting synaptic integration between graft and host.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalThe Chinese journal of physiology
Volume36
Issue number3
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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