Emphysema lung tissue gene expression profiling

Heiko A. Golpon, Christopher D. Coldren, Martin R. Zamora, Gregory P. Cosgrove, Mark D. Moore, Rubin M. Tuder, Mark W. Geraci, Norbert F. Voelkel

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Emphysema occurs in a subgroup of patients with chronic obstructive pulmonary disease and patients with the genetic defect of alpha 1-antitrypsin deficiency who have a smoking history of many years' duration. Emphysema is generally the result of a chronic and progressive destruction of the alveolar structures, which is believed to be driven by chronic inflammation, infections, oxidative stress, and an imbalance of protease and antiprotease activity. Here, we use microarray technology to characterize the gene expression profile of lung tissue samples obtained from patients with advanced emphysema and that obtained from healthy subjects. We hypothesized that the gene expression profile of emphysema lung tissue is distinct when compared with the expression profile of normal lungs. We report that severely emphysematous tissue is characterized by a global decrease in gene expression and by an increased abundance of transcripts encoding proteins involved in inflammation, immune responses, and proteolysis. Whereas the gene expression profile is to some degree shared between "usual" emphysema and alpha1-antitrypsin deficiency-related emphysema, there are statistically significant differences in the modulation of groups of genes associated with protein and energy metabolism, and immune function, which allow distinction between these two emphysema types on the lung tissue level.

Original languageEnglish (US)
Pages (from-to)595-600
Number of pages6
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume31
Issue number6
DOIs
StatePublished - Dec 2004
Externally publishedYes

Fingerprint

Emphysema
Gene Expression Profiling
Gene expression
Tissue
Lung
Transcriptome
Proteolysis
alpha 1-Antitrypsin
Pulmonary diseases
Oxidative stress
Microarrays
Protease Inhibitors
Proteins
alpha 1-Antitrypsin Deficiency
Peptide Hydrolases
Inflammation
Genes
Modulation
Defects
Chronic Obstructive Pulmonary Disease

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Golpon, H. A., Coldren, C. D., Zamora, M. R., Cosgrove, G. P., Moore, M. D., Tuder, R. M., ... Voelkel, N. F. (2004). Emphysema lung tissue gene expression profiling. American Journal of Respiratory Cell and Molecular Biology, 31(6), 595-600. https://doi.org/10.1165/rcmb.2004-0008OC

Emphysema lung tissue gene expression profiling. / Golpon, Heiko A.; Coldren, Christopher D.; Zamora, Martin R.; Cosgrove, Gregory P.; Moore, Mark D.; Tuder, Rubin M.; Geraci, Mark W.; Voelkel, Norbert F.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 31, No. 6, 12.2004, p. 595-600.

Research output: Contribution to journalArticle

Golpon, HA, Coldren, CD, Zamora, MR, Cosgrove, GP, Moore, MD, Tuder, RM, Geraci, MW & Voelkel, NF 2004, 'Emphysema lung tissue gene expression profiling', American Journal of Respiratory Cell and Molecular Biology, vol. 31, no. 6, pp. 595-600. https://doi.org/10.1165/rcmb.2004-0008OC
Golpon HA, Coldren CD, Zamora MR, Cosgrove GP, Moore MD, Tuder RM et al. Emphysema lung tissue gene expression profiling. American Journal of Respiratory Cell and Molecular Biology. 2004 Dec;31(6):595-600. https://doi.org/10.1165/rcmb.2004-0008OC
Golpon, Heiko A. ; Coldren, Christopher D. ; Zamora, Martin R. ; Cosgrove, Gregory P. ; Moore, Mark D. ; Tuder, Rubin M. ; Geraci, Mark W. ; Voelkel, Norbert F. / Emphysema lung tissue gene expression profiling. In: American Journal of Respiratory Cell and Molecular Biology. 2004 ; Vol. 31, No. 6. pp. 595-600.
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