En bloc staining of bone under load does not improve dye diffusion into microcracks

David B. Burr, Charles H. Turner, Pratap Naick, Mark R. Forwood, Ramana Pidaparti

Research output: Contribution to journalArticle

10 Scopus citations


Microdamage accumulation in bone has been implicated in the pathogenesis of some bone fractures, and in implant loosening. Standard techniques for staining microcracks may not allow all cracks to be stained. We tested the hypothesis that crack closure in bone cortices after removal of a bending load may prevent diffusion of stain to sites of microcrack nucleation. Following cyclic loading, 26 canine femurs were divided into a group stained en bloc while applying a four point bending load, and another group stained without an applied load. No differences in number or length of microcracks were observed, indicating that crack closure does not prevent diffusion of stain to the crack location. Staining under load is unnecessary.

Original languageEnglish (US)
Pages (from-to)285-288
Number of pages4
JournalJournal of Biomechanics
Issue number3
StatePublished - Dec 19 1997


  • Basic fuchsin
  • Bone
  • Fatigue
  • Microcrack

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Fingerprint Dive into the research topics of 'En bloc staining of bone under load does not improve dye diffusion into microcracks'. Together they form a unique fingerprint.

  • Cite this