Endocytosis of aminoglycoside antibiotics in sensory hair cells

Eri Hashino, Marlene Shero

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

Immuno-gold electron microscopy was used to assess the uptake pathways of aminoglycoside antibiotic kanamycin (KM) in sensory hair cells. Accumulation of gold particles was evident on the plasma membrane as well as in large smooth vesicles beneath the apical surfaces of hair cells 12 h after a systemic administration of KM. Immuno-gold was exclusively localized in the vesicles 27 h post-injection. Cationic ferritin, a membrane-bound insoluble marker, was colocalized with KM in the vesicle structures after their simultaneous in vitro application. These results strongly suggest that KM is taken up into sensory hair cells via receptor-mediated endocytosis at their apical surfaces. In addition, the profound time lag between KM uptake and hair cell death suggests involvement of targeting mechanisms in cytotoxic signalling pathways of the drugs.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalBrain research
Volume704
Issue number1
DOIs
StatePublished - Dec 15 1995
Externally publishedYes

Keywords

  • Aminoglycoside
  • Cationic ferritin
  • Chicken
  • Endocytosis
  • Hair cell
  • Immuno-gold
  • Toxicity

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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