Endogenous adipose-derived factors diminish coronary endothelial function via Inhibition of nitric oxide synthase

Gregory A. Payne, Lena Borbouse, Ian N. Bratz, William C. Roell, H. Bohlen, Gregory M. Dick, Johnathan Tune

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Adipocytokines may be the molecular link between obesity and vascular disease. However, the effects of these factors on coronary vascular function have not been discerned. Accordingly, the goal of this investigation was to delineate the mechanisms by which endogenous adipose-derived factors affect coronary vascular endothelial function. Both isolated canine coronary arteries and coronary blood flow in anesthetized dogs were studied with and without exposure to adipose tissue. Infusion of adipose-conditioned buffer directly into the coronary circulation did not change baseline hemodynamics; however, endothelial-dependent vasodilation to bradykinin was impaired both in vitro and in vivo. Coronary vasodilation to sodium nitroprusside was unaltered by adipose tissue. Oxygen radical formation did not cause the impairment because quantified dihydroethidium staining was decreased by adipose tissue and neither a superoxide dismutase mimetic nor catalase improved endothelial function. Inhibition of nitric oxide (NO) synthase with L-NAME diminished bradykinin-mediated relaxations and eliminated the subsequent vascular effects of adipose tissue. In vitro measurement of NO demonstrated that adipose tissue exposure quickly lowered baseline NO and abolished bradykinin-induced NO production. The results indicate that adipose tissue releases factor(s) that selectively impair endothelial-dependent dilation via inhibition of NO synthase-mediated NO production.

Original languageEnglish
Pages (from-to)417-426
Number of pages10
JournalMicrocirculation
Volume15
Issue number5
DOIs
StatePublished - Jul 2008

Fingerprint

Nitric Oxide Synthase
Adipose Tissue
Nitric Oxide
Bradykinin
Blood Vessels
Vasodilation
Coronary Circulation
Adipokines
NG-Nitroarginine Methyl Ester
Thromboplastin
Nitroprusside
Vascular Diseases
Catalase
Superoxide Dismutase
Canidae
Dilatation
Reactive Oxygen Species
Coronary Vessels
Buffers
Obesity

Keywords

  • Adipocytokine
  • Coronary circulation
  • Nitric oxide
  • Perivascular adipose tissue
  • Vascular endothelium

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Cardiology and Cardiovascular Medicine

Cite this

Endogenous adipose-derived factors diminish coronary endothelial function via Inhibition of nitric oxide synthase. / Payne, Gregory A.; Borbouse, Lena; Bratz, Ian N.; Roell, William C.; Bohlen, H.; Dick, Gregory M.; Tune, Johnathan.

In: Microcirculation, Vol. 15, No. 5, 07.2008, p. 417-426.

Research output: Contribution to journalArticle

Payne, Gregory A. ; Borbouse, Lena ; Bratz, Ian N. ; Roell, William C. ; Bohlen, H. ; Dick, Gregory M. ; Tune, Johnathan. / Endogenous adipose-derived factors diminish coronary endothelial function via Inhibition of nitric oxide synthase. In: Microcirculation. 2008 ; Vol. 15, No. 5. pp. 417-426.
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